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Evaluation of the production of exopolysaccharides by two strains of the thermophilic bacterium Rhodothermus marinus

Sardari, Roya R R LU ; Kulcinskaja, Evelina LU ; Ron, Emanuel Y C LU ; Björnsdóttir, Snædís; Friðjónsson, Ólafur H.; Hreggviðsson, Guðmundur Óli and Karlsson, Eva Nordberg LU (2017) In Carbohydrate Polymers 156. p.1-8
Abstract

The thermophile Rhodothermus marinus produces extracellular polysaccharides (EPSs) that forms a distinct cellular capsule. Here, the first data on EPS production in strains DSM4252T and MAT493 are reported and compared. Cultures of both strains, supplemented with either glucose, sucrose, lactose or maltose showed that the EPS were produced both in the exponential and stationary growth phase and that production in the exponential phase was boosted by maltose supplementation, while stationary phase production was boosted by lactose. The latter was higher, resulting in 8.8 (DSM4252T) and 13.7 mg EPS/g cell dry weight (MAT493) in cultures in marine broth supplemented with 10 g/L lactose. The EPSs were heteropolymeric... (More)

The thermophile Rhodothermus marinus produces extracellular polysaccharides (EPSs) that forms a distinct cellular capsule. Here, the first data on EPS production in strains DSM4252T and MAT493 are reported and compared. Cultures of both strains, supplemented with either glucose, sucrose, lactose or maltose showed that the EPS were produced both in the exponential and stationary growth phase and that production in the exponential phase was boosted by maltose supplementation, while stationary phase production was boosted by lactose. The latter was higher, resulting in 8.8 (DSM4252T) and 13.7 mg EPS/g cell dry weight (MAT493) in cultures in marine broth supplemented with 10 g/L lactose. The EPSs were heteropolymeric with an average molecular weight of 8 × 104 Da and different monosaccharides, including arabinose and xylose. FT-IR spectroscopy revealed presence of hydroxyl, carboxyl, N-acetyl, amine, and sulfate ester groups, showing that R. marinus produces unusual sulfated EPS with high arabinose and xylose content.

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author
organization
publishing date
type
Contribution to journal
publication status
epub
subject
keywords
EPS, Exopolysaccharide, Heteropolymer, Rhodothermus marinus
in
Carbohydrate Polymers
volume
156
pages
8 pages
publisher
Elsevier
external identifiers
  • Scopus:84985960370
ISSN
0144-8617
DOI
10.1016/j.carbpol.2016.08.062
language
English
LU publication?
yes
id
71ae6ac1-39a0-4259-8d20-f764fa5cec57
date added to LUP
2016-09-20 14:43:30
date last changed
2016-09-29 12:13:57
@misc{71ae6ac1-39a0-4259-8d20-f764fa5cec57,
  abstract     = {<p>The thermophile Rhodothermus marinus produces extracellular polysaccharides (EPSs) that forms a distinct cellular capsule. Here, the first data on EPS production in strains DSM4252<sup>T</sup> and MAT493 are reported and compared. Cultures of both strains, supplemented with either glucose, sucrose, lactose or maltose showed that the EPS were produced both in the exponential and stationary growth phase and that production in the exponential phase was boosted by maltose supplementation, while stationary phase production was boosted by lactose. The latter was higher, resulting in 8.8 (DSM4252<sup>T</sup>) and 13.7 mg EPS/g cell dry weight (MAT493) in cultures in marine broth supplemented with 10 g/L lactose. The EPSs were heteropolymeric with an average molecular weight of 8 × 10<sup>4</sup> Da and different monosaccharides, including arabinose and xylose. FT-IR spectroscopy revealed presence of hydroxyl, carboxyl, N-acetyl, amine, and sulfate ester groups, showing that R. marinus produces unusual sulfated EPS with high arabinose and xylose content.</p>},
  author       = {Sardari, Roya R R and Kulcinskaja, Evelina and Ron, Emanuel Y C and Björnsdóttir, Snædís and Friðjónsson, Ólafur H. and Hreggviðsson, Guðmundur Óli and Karlsson, Eva Nordberg},
  issn         = {0144-8617},
  keyword      = {EPS,Exopolysaccharide,Heteropolymer,Rhodothermus marinus},
  language     = {eng},
  month        = {01},
  pages        = {1--8},
  publisher    = {ARRAY(0x9455828)},
  series       = {Carbohydrate Polymers},
  title        = {Evaluation of the production of exopolysaccharides by two strains of the thermophilic bacterium Rhodothermus marinus},
  url          = {http://dx.doi.org/10.1016/j.carbpol.2016.08.062},
  volume       = {156},
  year         = {2017},
}