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An anesthetic method compatible with (18)F-FDG-PET studies in mice.

Siikanen, Jonathan LU ; Sjövall, Johanna LU ; Forslid, Anders LU ; Brun, Eva LU ; Bjurberg, Maria LU ; Wennerberg, Johan LU ; Ekblad, Lars LU and Sandell, Anders LU (2015) In American journal of nuclear medicine and molecular imaging 5(3). p.270-277
Abstract
The purpose of this study was to establish an experimental setting and an anesthetic method compatible with future sequential studies using (18)F-FDG-PET single scans, i.e. autoradiographic measurements, for the estimation of metabolic rate of glucose (MRglc) in mice. In this study we had no access to a small animal PET scanner and therefore focus was on the anesthetic setting and optimization of the input function as a preparation for the future tumor metabolic studies. Initially, four combinations of intraperitoneal (ip) anesthesia were tested on tumor bearing mice. Fentanyl-fluanisone plus diazepam yielded low and stable blood glucose levels and kept the animals sedated for approximately 2 h. The anesthesia was also tested in a... (More)
The purpose of this study was to establish an experimental setting and an anesthetic method compatible with future sequential studies using (18)F-FDG-PET single scans, i.e. autoradiographic measurements, for the estimation of metabolic rate of glucose (MRglc) in mice. In this study we had no access to a small animal PET scanner and therefore focus was on the anesthetic setting and optimization of the input function as a preparation for the future tumor metabolic studies. Initially, four combinations of intraperitoneal (ip) anesthesia were tested on tumor bearing mice. Fentanyl-fluanisone plus diazepam yielded low and stable blood glucose levels and kept the animals sedated for approximately 2 h. The anesthesia was also tested in a longitudinal (18)F-FDG study, where tumor bearing mice were anesthetized, injected with (18)F-FDG, and sampled for blood, before, one day after, and 8 days after treatment with cisplatin. The animals were in good condition during the entire study period. To validate the method, average MRglc of whole brain and cerebellum in mice were calculated and compared with the literature. The average MRglc in the whole brain and cerebellum were 46.2±4.4 and 39.0±3.1 µmol 100g(-1) min(-1). In the present study, we have shown that an ip anesthesia with a combination of fentanyl-fluanisone and diazepam is feasible and provides stable and low blood glucose levels after a fasting period of 4 h in experiments in nude mice with xenografted human tumors. We have also verified that (18)F-FDG, intraperitoneally administrated, results in an expected plasma activity uptake and clearance. The method doesn't alter the uptake in brain which is an indirect indication that the anesthesia doesn't alter the uptake in other organs. In combination with meticulous animal handling this set-up is reliable and future sequential tumor studies of early metabolic effects with calculation of MRglc following cytotoxic therapy are made possible. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
American journal of nuclear medicine and molecular imaging
volume
5
issue
3
pages
270 - 277
publisher
e-Century Publishing Corporation
external identifiers
  • PMID:26069860
ISSN
2160-8407
language
English
LU publication?
yes
id
02cadf20-9dc9-425f-9b45-91b7cd85d1e9 (old id 7486486)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26069860?dopt=Abstract
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4446395/
date added to LUP
2015-07-08 13:17:39
date last changed
2016-04-16 06:43:54
@misc{02cadf20-9dc9-425f-9b45-91b7cd85d1e9,
  abstract     = {The purpose of this study was to establish an experimental setting and an anesthetic method compatible with future sequential studies using (18)F-FDG-PET single scans, i.e. autoradiographic measurements, for the estimation of metabolic rate of glucose (MRglc) in mice. In this study we had no access to a small animal PET scanner and therefore focus was on the anesthetic setting and optimization of the input function as a preparation for the future tumor metabolic studies. Initially, four combinations of intraperitoneal (ip) anesthesia were tested on tumor bearing mice. Fentanyl-fluanisone plus diazepam yielded low and stable blood glucose levels and kept the animals sedated for approximately 2 h. The anesthesia was also tested in a longitudinal (18)F-FDG study, where tumor bearing mice were anesthetized, injected with (18)F-FDG, and sampled for blood, before, one day after, and 8 days after treatment with cisplatin. The animals were in good condition during the entire study period. To validate the method, average MRglc of whole brain and cerebellum in mice were calculated and compared with the literature. The average MRglc in the whole brain and cerebellum were 46.2±4.4 and 39.0±3.1 µmol 100g(-1) min(-1). In the present study, we have shown that an ip anesthesia with a combination of fentanyl-fluanisone and diazepam is feasible and provides stable and low blood glucose levels after a fasting period of 4 h in experiments in nude mice with xenografted human tumors. We have also verified that (18)F-FDG, intraperitoneally administrated, results in an expected plasma activity uptake and clearance. The method doesn't alter the uptake in brain which is an indirect indication that the anesthesia doesn't alter the uptake in other organs. In combination with meticulous animal handling this set-up is reliable and future sequential tumor studies of early metabolic effects with calculation of MRglc following cytotoxic therapy are made possible.},
  author       = {Siikanen, Jonathan and Sjövall, Johanna and Forslid, Anders and Brun, Eva and Bjurberg, Maria and Wennerberg, Johan and Ekblad, Lars and Sandell, Anders},
  issn         = {2160-8407},
  language     = {eng},
  number       = {3},
  pages        = {270--277},
  publisher    = {ARRAY(0xc6e6128)},
  series       = {American journal of nuclear medicine and molecular imaging},
  title        = {An anesthetic method compatible with (18)F-FDG-PET studies in mice.},
  volume       = {5},
  year         = {2015},
}