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Selection of antibodies for intracellular function using a two-hybrid in vivo system

Visintin, M; Tse, E; Axelson, H LU ; Rabbitts, T H and Cattaneo, A (1999) In Proceedings of the National Academy of Sciences 96(21). p.11723-8
Abstract

Expression of antibodies inside cells has been used successfully to ablate protein function. This finding suggests that the technology should have an impact on disease treatment and in functional genomics where proteins of unknown function are predicted from genomic sequences. A major hindrance is the paucity of antibodies that function in eukaryotic cells, presumably because the antibodies fold incorrectly in the cytoplasm. To overcome this problem, we have developed an in vivo assay for functional intracellular antibodies using a two-hybrid approach. In this assay, antibody, as single-chain Fv (scFv) linked to a transcriptional transactivation domain, can interact with a target antigen, linked to a LexA-DNA binding domain, and thereby... (More)

Expression of antibodies inside cells has been used successfully to ablate protein function. This finding suggests that the technology should have an impact on disease treatment and in functional genomics where proteins of unknown function are predicted from genomic sequences. A major hindrance is the paucity of antibodies that function in eukaryotic cells, presumably because the antibodies fold incorrectly in the cytoplasm. To overcome this problem, we have developed an in vivo assay for functional intracellular antibodies using a two-hybrid approach. In this assay, antibody, as single-chain Fv (scFv) linked to a transcriptional transactivation domain, can interact with a target antigen, linked to a LexA-DNA binding domain, and thereby activate a reporter gene. We find that several characterized antibodies can bind their target antigen in eukaryotic cells in this two-hybrid format, and we have been able to isolate intracellular binders from among sets of scFv that can bind antigen in vitro. Furthermore, we show a model selection in which a single scFv was isolated from a mixture of half a million clones, indicating that this is a robust procedure that should facilitate capture of antibody specificities from complex mixtures. The approach can provide the basis for de novo selection of intracellular scFv from libraries, such as those made from spleen RNA after immunization with antigen, for intracellular analysis of protein function based only on genomic or cDNA sequences.

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author
organization
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type
Contribution to journal
publication status
published
keywords
Animals, Antibody Affinity, Antigen-Antibody Reactions, Blotting, Western, CHO Cells, Chloramphenicol O-Acetyltransferase, Cricetinae, Disulfides, Genes, Reporter, Immunoglobulin Fragments, Models, Immunological, Saccharomyces cerevisiae, Transfection, Transformation, Genetic, Two-Hybrid System Techniques
in
Proceedings of the National Academy of Sciences
volume
96
issue
21
pages
11723 - 8
publisher
National Academy of Sciences
external identifiers
  • Scopus:0032716364
ISSN
0027-8424
DOI
10.1073/pnas.96.21.11723
language
English
LU publication?
yes
id
825748d9-a662-42fa-ab75-bbc01fdb7c8f
date added to LUP
2016-08-09 09:10:33
date last changed
2016-09-20 03:05:34
@misc{825748d9-a662-42fa-ab75-bbc01fdb7c8f,
  abstract     = {<p>Expression of antibodies inside cells has been used successfully to ablate protein function. This finding suggests that the technology should have an impact on disease treatment and in functional genomics where proteins of unknown function are predicted from genomic sequences. A major hindrance is the paucity of antibodies that function in eukaryotic cells, presumably because the antibodies fold incorrectly in the cytoplasm. To overcome this problem, we have developed an in vivo assay for functional intracellular antibodies using a two-hybrid approach. In this assay, antibody, as single-chain Fv (scFv) linked to a transcriptional transactivation domain, can interact with a target antigen, linked to a LexA-DNA binding domain, and thereby activate a reporter gene. We find that several characterized antibodies can bind their target antigen in eukaryotic cells in this two-hybrid format, and we have been able to isolate intracellular binders from among sets of scFv that can bind antigen in vitro. Furthermore, we show a model selection in which a single scFv was isolated from a mixture of half a million clones, indicating that this is a robust procedure that should facilitate capture of antibody specificities from complex mixtures. The approach can provide the basis for de novo selection of intracellular scFv from libraries, such as those made from spleen RNA after immunization with antigen, for intracellular analysis of protein function based only on genomic or cDNA sequences.</p>},
  author       = {Visintin, M and Tse, E and Axelson, H and Rabbitts, T H and Cattaneo, A},
  issn         = {0027-8424},
  keyword      = {Animals,Antibody Affinity,Antigen-Antibody Reactions,Blotting, Western,CHO Cells,Chloramphenicol O-Acetyltransferase,Cricetinae,Disulfides,Genes, Reporter,Immunoglobulin Fragments,Models, Immunological,Saccharomyces cerevisiae,Transfection,Transformation, Genetic,Two-Hybrid System Techniques},
  language     = {eng},
  month        = {10},
  number       = {21},
  pages        = {11723--8},
  publisher    = {ARRAY(0x909cf70)},
  series       = {Proceedings of the National Academy of Sciences},
  title        = {Selection of antibodies for intracellular function using a two-hybrid in vivo system},
  url          = {http://dx.doi.org/    10.1073/pnas.96.21.11723},
  volume       = {96},
  year         = {1999},
}