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Multiple cryptic species of sympatric generalists within the avian blood parasite Haemoproteus majoris

Nilsson, E. LU ; Taubert, H.; Hellgren, O. LU ; Huang, X. LU ; Palinauskas, V.; Markovets, M. Y.; Valkiūnas, G. and Bensch, S. LU (2016) In Journal of Evolutionary Biology 29(9). p.1812-1826
Abstract

The avian haemosporidian parasite Haemoproteus majoris has been reported to infect a wide range of passerine birds throughout the Holarctic ecozone. Five cytochrome b (cyt b) lineages have been described as belonging to the morphological species H. majoris, and these form a tight phylogenetic cluster together with 13 undescribed lineages that differ from each other by < 1.2% in sequence divergence. Records in a database (MalAvi) that contains global findings of haemosporidian lineages generated by universal primers suggest that these lineages vary substantially in host distribution. We confirm this pattern in a data set collected at Lake Kvismaren, Sweden, where three of the generalist lineages have local transmission. However,... (More)

The avian haemosporidian parasite Haemoproteus majoris has been reported to infect a wide range of passerine birds throughout the Holarctic ecozone. Five cytochrome b (cyt b) lineages have been described as belonging to the morphological species H. majoris, and these form a tight phylogenetic cluster together with 13 undescribed lineages that differ from each other by < 1.2% in sequence divergence. Records in a database (MalAvi) that contains global findings of haemosporidian lineages generated by universal primers suggest that these lineages vary substantially in host distribution. We confirm this pattern in a data set collected at Lake Kvismaren, Sweden, where three of the generalist lineages have local transmission. However, whether these lineages represent intraspecific mitochondrial diversity or clusters of cryptic species has previously not been examined. In this study, we developed novel molecular markers to amplify the partial segments of four nuclear genes to determine the level of genetic diversity and gene phylogenies among the five morphologically described cyt b lineages of H. majoris. All five cyt b lineages were strongly associated with unique nuclear alleles at all four nuclear loci, indicating that each mitochondrial lineage represents a distinct biological species. Within lineages, there was no apparent association between nuclear alleles and host species, indicating that they form genetically unstructured populations across multiple host species.

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Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cytochrome b lineages, Genetic diversity, Haemosporidian parasites, Host range, Host specificity
in
Journal of Evolutionary Biology
volume
29
issue
9
pages
15 pages
publisher
John Wiley & Sons
external identifiers
  • Scopus:84977104102
  • Scopus:84986888986
ISSN
1010-061X
DOI
10.1111/jeb.12911http://dx.doi.org/10.1111/jeb.12911
language
English
LU publication?
yes
id
b6ab5d91-bd08-412b-b55d-cca53d0e1720
date added to LUP
2016-09-22 10:52:59
date last changed
2016-11-23 10:59:55
@misc{b6ab5d91-bd08-412b-b55d-cca53d0e1720,
  abstract     = {<p>The avian haemosporidian parasite Haemoproteus majoris has been reported to infect a wide range of passerine birds throughout the Holarctic ecozone. Five cytochrome b (cyt b) lineages have been described as belonging to the morphological species H. majoris, and these form a tight phylogenetic cluster together with 13 undescribed lineages that differ from each other by &lt; 1.2% in sequence divergence. Records in a database (MalAvi) that contains global findings of haemosporidian lineages generated by universal primers suggest that these lineages vary substantially in host distribution. We confirm this pattern in a data set collected at Lake Kvismaren, Sweden, where three of the generalist lineages have local transmission. However, whether these lineages represent intraspecific mitochondrial diversity or clusters of cryptic species has previously not been examined. In this study, we developed novel molecular markers to amplify the partial segments of four nuclear genes to determine the level of genetic diversity and gene phylogenies among the five morphologically described cyt b lineages of H. majoris. All five cyt b lineages were strongly associated with unique nuclear alleles at all four nuclear loci, indicating that each mitochondrial lineage represents a distinct biological species. Within lineages, there was no apparent association between nuclear alleles and host species, indicating that they form genetically unstructured populations across multiple host species.</p>},
  author       = {Nilsson, E. and Taubert, H. and Hellgren, O. and Huang, X. and Palinauskas, V. and Markovets, M. Y. and Valkiūnas, G. and Bensch, S.},
  issn         = {1010-061X},
  keyword      = {Cytochrome b lineages,Genetic diversity,Haemosporidian parasites,Host range,Host specificity},
  language     = {eng},
  month        = {09},
  number       = {9},
  pages        = {1812--1826},
  publisher    = {ARRAY(0xa7b7108)},
  series       = {Journal of Evolutionary Biology},
  title        = {Multiple cryptic species of sympatric generalists within the avian blood parasite Haemoproteus majoris},
  url          = {http://dx.doi.org/10.1111/jeb.12911http://dx.doi.org/10.1111/jeb.12911},
  volume       = {29},
  year         = {2016},
}