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The effect of Cobalt on mitochondrial ATP-production in the rat myocardium and skeletal muscle

Clyne, N. LU ; Wibom, R.; Havu, N.; Hultman, E.; Pehrsson, S. K. and Persson, B. (1990) In Scandinavian Journal of Clinical & Laboratory Investigation 50(2). p.153-159
Abstract

Cobalt has been shown to accumulate in the myocardium of uraemic patients and has been suggested as a myocardial toxin inhibiting mitochondrial respiration. In order to study the cellular effects of cobalt exposure three groups of rats (n=12 per group) were fed a diet containing 12% protein without supplementation or with 20 mg and 40 mg CoSO4 7 H2O/kg body weight/day respectively. After 8 weeks the hearts and soleus muscles were removed. Cobalt in tissues and in four cell fractions were analysed with neutron-activation analysis (ng/g wet weight and ng/mg protein respectively). Mitochondrial respiration was analysed as ATP-production rate using pyruvate+malate and palmitoyl-carnitine+malate as substrate. The... (More)

Cobalt has been shown to accumulate in the myocardium of uraemic patients and has been suggested as a myocardial toxin inhibiting mitochondrial respiration. In order to study the cellular effects of cobalt exposure three groups of rats (n=12 per group) were fed a diet containing 12% protein without supplementation or with 20 mg and 40 mg CoSO4 7 H2O/kg body weight/day respectively. After 8 weeks the hearts and soleus muscles were removed. Cobalt in tissues and in four cell fractions were analysed with neutron-activation analysis (ng/g wet weight and ng/mg protein respectively). Mitochondrial respiration was analysed as ATP-production rate using pyruvate+malate and palmitoyl-carnitine+malate as substrate. The ATP-production from pyruvate+ malate was unchanged in both heart and skeletal muscle in the exposed animals. With palmitate as substrate, the heart muscle showed a slightly lower ATP-production rate (p

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Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
keywords
Cobalt, Mitochondrial ATP-production, Rat myocardium, Skeletal muscle
in
Scandinavian Journal of Clinical & Laboratory Investigation
volume
50
issue
2
pages
7 pages
publisher
Informa Healthcare
external identifiers
  • Scopus:0025260789
ISSN
0036-5513
DOI
10.3109/00365519009089147
language
English
LU publication?
no
id
b7240349-08ce-472e-93ac-e5a9c6deaf3b
date added to LUP
2016-08-30 22:06:47
date last changed
2016-09-16 15:09:51
@misc{b7240349-08ce-472e-93ac-e5a9c6deaf3b,
  abstract     = {<p>Cobalt has been shown to accumulate in the myocardium of uraemic patients and has been suggested as a myocardial toxin inhibiting mitochondrial respiration. In order to study the cellular effects of cobalt exposure three groups of rats (n=12 per group) were fed a diet containing 12% protein without supplementation or with 20 mg and 40 mg CoSO<sub>4</sub> 7 H<sub>2</sub>O/kg body weight/day respectively. After 8 weeks the hearts and soleus muscles were removed. Cobalt in tissues and in four cell fractions were analysed with neutron-activation analysis (ng/g wet weight and ng/mg protein respectively). Mitochondrial respiration was analysed as ATP-production rate using pyruvate+malate and palmitoyl-carnitine+malate as substrate. The ATP-production from pyruvate+ malate was unchanged in both heart and skeletal muscle in the exposed animals. With palmitate as substrate, the heart muscle showed a slightly lower ATP-production rate (p</p>},
  author       = {Clyne, N. and Wibom, R. and Havu, N. and Hultman, E. and Pehrsson, S. K. and Persson, B.},
  issn         = {0036-5513},
  keyword      = {Cobalt,Mitochondrial ATP-production,Rat myocardium,Skeletal muscle},
  language     = {eng},
  number       = {2},
  pages        = {153--159},
  publisher    = {ARRAY(0x876b4f8)},
  series       = {Scandinavian Journal of Clinical & Laboratory Investigation},
  title        = {The effect of Cobalt on mitochondrial ATP-production in the rat myocardium and skeletal muscle},
  url          = {http://dx.doi.org/10.3109/00365519009089147},
  volume       = {50},
  year         = {1990},
}