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Impaired phagocytosis and reactive oxygen species production in phagocytes is associated with systemic vasculitis

Johansson, Åsa Cm LU ; Ohlsson, Sophie LU ; Pettersson, Åsa LU ; Bengtsson, Anders LU ; Selga, Daina LU ; Hansson, Markus LU and Hellmark, Thomas LU (2015) In Arthritis Research & Therapy 18. p.92-92
Abstract

BACKGROUND: Anti-neutrophil cytoplasmic antibodies associated vasculitides (AAV) is a group of autoimmune diseases, characterized by small vessel inflammation. Phagocytes such as neutrophils and monocytes are the main effector cells found around the inflamed vessel wall. Therefore, we wanted to investigate aspects of function and activation of these cells in patients with AAV.

METHODS: Flow cytometry was used to evaluate: the expression of activation markers (CD11c, CD62L, CD177 and C5aR); the number of recently released neutrophils from bone marrow, defined as CD10(-)D16(low) cells in peripheral blood; and the capacity of peripheral blood monocytes and polymorphonuclear leukocytes (PMN) to produce reactive oxygen species and to... (More)

BACKGROUND: Anti-neutrophil cytoplasmic antibodies associated vasculitides (AAV) is a group of autoimmune diseases, characterized by small vessel inflammation. Phagocytes such as neutrophils and monocytes are the main effector cells found around the inflamed vessel wall. Therefore, we wanted to investigate aspects of function and activation of these cells in patients with AAV.

METHODS: Flow cytometry was used to evaluate: the expression of activation markers (CD11c, CD62L, CD177 and C5aR); the number of recently released neutrophils from bone marrow, defined as CD10(-)D16(low) cells in peripheral blood; and the capacity of peripheral blood monocytes and polymorphonuclear leukocytes (PMN) to produce reactive oxygen species and to phagocytose opsonized bacteria.

RESULTS: AAV patients (n = 104) showed an increase of CD10(-)CD16(low) neutrophils and total PMN in peripheral blood, suggesting a combination of increased bone marrow release and prolonged survival. An increased percentage of AAV PMN expressed CD177 but no other signs of activation were seen. A decreased production of reactive oxygen species was observed in AAV phagocytes, which was associated with disease activity. Moreover, granulocytes from patients with microscopic polyangiitis showed lower oxidative burst capacity compared to patients with granulomatosis with polyangiitis or eosinophilic granulomatosis with polyangiitis. In addition, decreased phagocytosis capacity was seen in PMN and monocytes.

CONCLUSION: Our results indicate that phagocytes from AAV patients have impaired function, are easily mobilized from bone marrow but are not particularly activated. The association between low reactive oxygen species formation in PMN and disease severity is consistent with findings in other autoimmune diseases and might be considered as a risk factor.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Journal Article, Research Support, Non-U.S. Gov't
in
Arthritis Research & Therapy
volume
18
pages
92 - 92
publisher
BioMed Central
external identifiers
  • Scopus:84977529632
ISSN
1478-6354
DOI
10.1186/s13075-016-0994-1
language
English
LU publication?
yes
id
c76f1777-9280-4b13-afc4-92fb585ec644
date added to LUP
2016-09-19 10:46:13
date last changed
2016-11-10 14:18:37
@misc{c76f1777-9280-4b13-afc4-92fb585ec644,
  abstract     = {<p>BACKGROUND: Anti-neutrophil cytoplasmic antibodies associated vasculitides (AAV) is a group of autoimmune diseases, characterized by small vessel inflammation. Phagocytes such as neutrophils and monocytes are the main effector cells found around the inflamed vessel wall. Therefore, we wanted to investigate aspects of function and activation of these cells in patients with AAV.</p><p>METHODS: Flow cytometry was used to evaluate: the expression of activation markers (CD11c, CD62L, CD177 and C5aR); the number of recently released neutrophils from bone marrow, defined as CD10(-)D16(low) cells in peripheral blood; and the capacity of peripheral blood monocytes and polymorphonuclear leukocytes (PMN) to produce reactive oxygen species and to phagocytose opsonized bacteria.</p><p>RESULTS: AAV patients (n = 104) showed an increase of CD10(-)CD16(low) neutrophils and total PMN in peripheral blood, suggesting a combination of increased bone marrow release and prolonged survival. An increased percentage of AAV PMN expressed CD177 but no other signs of activation were seen. A decreased production of reactive oxygen species was observed in AAV phagocytes, which was associated with disease activity. Moreover, granulocytes from patients with microscopic polyangiitis showed lower oxidative burst capacity compared to patients with granulomatosis with polyangiitis or eosinophilic granulomatosis with polyangiitis. In addition, decreased phagocytosis capacity was seen in PMN and monocytes.</p><p>CONCLUSION: Our results indicate that phagocytes from AAV patients have impaired function, are easily mobilized from bone marrow but are not particularly activated. The association between low reactive oxygen species formation in PMN and disease severity is consistent with findings in other autoimmune diseases and might be considered as a risk factor.</p>},
  author       = {Johansson, Åsa Cm and Ohlsson, Sophie and Pettersson, Åsa and Bengtsson, Anders and Selga, Daina and Hansson, Markus and Hellmark, Thomas},
  issn         = {1478-6354},
  keyword      = {Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  pages        = {92--92},
  publisher    = {ARRAY(0x4a9ab18)},
  series       = {Arthritis Research & Therapy},
  title        = {Impaired phagocytosis and reactive oxygen species production in phagocytes is associated with systemic vasculitis},
  url          = {http://dx.doi.org/10.1186/s13075-016-0994-1},
  volume       = {18},
  year         = {2015},
}