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Antisense repression of the mitochondrial nadh-binding subunit of complex I in transgenic potato plants affects male fertility

Heiser, Volker; Rasmusson, Allan G. LU ; Thieck, Oliver; Brennicke, Axel and Grohmann, Lutz (1997) In Plant Science 127(1). p.61-69
Abstract

Mitochondrial respiratory chain complex I is a large multi-subunit enzyme composed of both organellar and nuclear encoded proteins. To investigate the role of the nuclear encoded components, expression of the gene for the 55 kDa NADH-binding subunit of complex I was disturbed by antisense repression in transgenic potato plants. The antisense construct driven by the CaMV 35S promoter decreases the steady-state mRNA levels of transcripts for the 55 kDa subunit to 33% of the wild type levels. Quantities of the 55 kDa protein in mitochondrial protein extracts are lowered to about 50% in these plants. Transgenic plants show normal vegetative growth and tuber formation, but pollen maturation is found to be disturbed. The reduced male... (More)

Mitochondrial respiratory chain complex I is a large multi-subunit enzyme composed of both organellar and nuclear encoded proteins. To investigate the role of the nuclear encoded components, expression of the gene for the 55 kDa NADH-binding subunit of complex I was disturbed by antisense repression in transgenic potato plants. The antisense construct driven by the CaMV 35S promoter decreases the steady-state mRNA levels of transcripts for the 55 kDa subunit to 33% of the wild type levels. Quantities of the 55 kDa protein in mitochondrial protein extracts are lowered to about 50% in these plants. Transgenic plants show normal vegetative growth and tuber formation, but pollen maturation is found to be disturbed. The reduced male fertility of the transgenic 55 kDa antisense plants may be caused by an insufficient mitochondrial respiratory chain, impaired by the decreased expression of the NADH-binding component of mitochondrial complex I.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Antisense repression, Male fertility, Mitochondrial NADH-dehydrogenase (complex I), Solanum tuberosum
in
Plant Science
volume
127
issue
1
pages
9 pages
publisher
Elsevier
external identifiers
  • Scopus:0030835681
ISSN
0168-9452
DOI
10.1016/S0168-9452(97)00118-0
language
English
LU publication?
yes
id
c893d23c-6a89-45da-9be9-3cc6ccc86024
date added to LUP
2016-05-31 21:30:53
date last changed
2016-08-10 14:02:54
@misc{c893d23c-6a89-45da-9be9-3cc6ccc86024,
  abstract     = {<p>Mitochondrial respiratory chain complex I is a large multi-subunit enzyme composed of both organellar and nuclear encoded proteins. To investigate the role of the nuclear encoded components, expression of the gene for the 55 kDa NADH-binding subunit of complex I was disturbed by antisense repression in transgenic potato plants. The antisense construct driven by the CaMV 35S promoter decreases the steady-state mRNA levels of transcripts for the 55 kDa subunit to 33% of the wild type levels. Quantities of the 55 kDa protein in mitochondrial protein extracts are lowered to about 50% in these plants. Transgenic plants show normal vegetative growth and tuber formation, but pollen maturation is found to be disturbed. The reduced male fertility of the transgenic 55 kDa antisense plants may be caused by an insufficient mitochondrial respiratory chain, impaired by the decreased expression of the NADH-binding component of mitochondrial complex I.</p>},
  author       = {Heiser, Volker and Rasmusson, Allan G. and Thieck, Oliver and Brennicke, Axel and Grohmann, Lutz},
  issn         = {0168-9452},
  keyword      = {Antisense repression,Male fertility,Mitochondrial NADH-dehydrogenase (complex I),Solanum tuberosum},
  language     = {eng},
  month        = {08},
  number       = {1},
  pages        = {61--69},
  publisher    = {ARRAY(0xafbfb18)},
  series       = {Plant Science},
  title        = {Antisense repression of the mitochondrial nadh-binding subunit of complex I in transgenic potato plants affects male fertility},
  url          = {http://dx.doi.org/10.1016/S0168-9452(97)00118-0},
  volume       = {127},
  year         = {1997},
}