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A β-mannan utilisation locus in Bacteroides ovatus involves a GH36 α-galactosidase active on galactomannans

Krishnaswamyreddy, Sumitha LU ; Bågenholm, Viktoria LU ; Pudlo, Nicholas A; Bouraoui, Hanene; Koropatkin, Nicole M; Martens, Eric C and Stålbrand, Henrik LU (2016) In FEBS Letters
Abstract

The Bacova_02091 gene in the β-mannan utilisation locus of Bacteroides ovatus encodes a family GH36 α-galactosidase (BoGal36A), transcriptionally upregulated during growth on galactomannan. Characterisation of recombinant BoGal36A reveals unique properties compared to other GH36 α-galactosidases, which preferentially hydrolyse terminal α-galactose in raffinose family oligosaccharides. BoGal36A prefers hydrolysing internal galactose substitutions from intact and depolymerized galactomannan. BoGal36A efficiently releases (>90%) galactose from guar and locust bean galactomannans, resulting in precipitation of the polysaccharides. As compared to other GH36 structures, the BoGal36A 3D model displays a loop deletion, resulting in a wider... (More)

The Bacova_02091 gene in the β-mannan utilisation locus of Bacteroides ovatus encodes a family GH36 α-galactosidase (BoGal36A), transcriptionally upregulated during growth on galactomannan. Characterisation of recombinant BoGal36A reveals unique properties compared to other GH36 α-galactosidases, which preferentially hydrolyse terminal α-galactose in raffinose family oligosaccharides. BoGal36A prefers hydrolysing internal galactose substitutions from intact and depolymerized galactomannan. BoGal36A efficiently releases (>90%) galactose from guar and locust bean galactomannans, resulting in precipitation of the polysaccharides. As compared to other GH36 structures, the BoGal36A 3D model displays a loop deletion, resulting in a wider active site cleft which likely can accommodate a galactose-substituted polymannose backbone. This article is protected by copyright. All rights reserved.

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author
organization
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type
Contribution to journal
publication status
published
subject
in
FEBS Letters
publisher
Wiley-Blackwell
external identifiers
  • Scopus:84979600169
ISSN
1873-3468
DOI
10.1002/1873-3468.12250
language
English
LU publication?
yes
id
d21192c5-3456-4dcb-9fbd-36a34ee875bc
date added to LUP
2016-06-16 15:06:55
date last changed
2016-10-27 12:42:43
@misc{d21192c5-3456-4dcb-9fbd-36a34ee875bc,
  abstract     = {<p>The Bacova_02091 gene in the β-mannan utilisation locus of Bacteroides ovatus encodes a family GH36 α-galactosidase (BoGal36A), transcriptionally upregulated during growth on galactomannan. Characterisation of recombinant BoGal36A reveals unique properties compared to other GH36 α-galactosidases, which preferentially hydrolyse terminal α-galactose in raffinose family oligosaccharides. BoGal36A prefers hydrolysing internal galactose substitutions from intact and depolymerized galactomannan. BoGal36A efficiently releases (&gt;90%) galactose from guar and locust bean galactomannans, resulting in precipitation of the polysaccharides. As compared to other GH36 structures, the BoGal36A 3D model displays a loop deletion, resulting in a wider active site cleft which likely can accommodate a galactose-substituted polymannose backbone. This article is protected by copyright. All rights reserved.</p>},
  author       = {Krishnaswamyreddy, Sumitha and Bågenholm, Viktoria and Pudlo, Nicholas A and Bouraoui, Hanene and Koropatkin, Nicole M and Martens, Eric C and Stålbrand, Henrik},
  issn         = {1873-3468},
  language     = {eng},
  month        = {06},
  publisher    = {ARRAY(0xb110e18)},
  series       = {FEBS Letters},
  title        = {A β-mannan utilisation locus in Bacteroides ovatus involves a GH36 α-galactosidase active on galactomannans},
  url          = {http://dx.doi.org/10.1002/1873-3468.12250},
  year         = {2016},
}