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High frequency of multiple melanomas and breast and pancreas carcinomas in CDKN2A mutation-positive melanoma families

Borg, Åke LU ; Sandberg, T LU ; Nilsson, K LU ; Johannsson, O LU ; Klinker, M; Måsbäck, A LU ; Westerdahl, J LU ; Olsson, Håkan LU and Ingvar, C LU (2000) In Journal of the National Cancer Institute 92(15). p.6-1260
Abstract

BACKGROUND: : Inherited mutations in the CDKN2A tumor suppressor gene, which encodes the p16(INK4a) protein, and in the cyclin-dependent kinase 4 (CDK4) gene confer susceptibility to cutaneous malignant melanoma. We analyzed families with two or more cases of melanoma for germline mutations in CDKN2A and CDK4 to elucidate the contribution of these gene defects to familial malignant melanoma and to the occurrence of other cancer types.

METHODS: : The entire CDKN2A coding region and exon 2 of the CDK4 gene of an affected member of each of 52 families from southern Sweden with at least two cases of melanoma in first- or second-degree relatives were screened for mutations by use of polymerase chain reaction-single-strand conformation... (More)

BACKGROUND: : Inherited mutations in the CDKN2A tumor suppressor gene, which encodes the p16(INK4a) protein, and in the cyclin-dependent kinase 4 (CDK4) gene confer susceptibility to cutaneous malignant melanoma. We analyzed families with two or more cases of melanoma for germline mutations in CDKN2A and CDK4 to elucidate the contribution of these gene defects to familial malignant melanoma and to the occurrence of other cancer types.

METHODS: : The entire CDKN2A coding region and exon 2 of the CDK4 gene of an affected member of each of 52 families from southern Sweden with at least two cases of melanoma in first- or second-degree relatives were screened for mutations by use of polymerase chain reaction-single-strand conformation polymorphism analysis. Statistical tests were two-sided.

RESULTS: : CDKN2A mutations were found in 10 (19%) of the 52 families. Nine families carried an identical alteration consisting of the insertion of arginine at position 113 of p16(INK4a), and one carried a missense mutation, in which the valine at position 115 was replaced with a glycine. The 113insArg mutant p16(INK4a) was unable to bind cdk4 and cdk6 in an in vitro binding assay. Six of the 113insArg families had at least one member with multiple primary melanomas; the 113insArg families also had a high frequency of other malignancies-in particular, breast cancer (a total of eight cases compared with the expected 2.1; P =.0014) and pancreatic cancer (a total of six cases compared with the expected 0.16; P<.0001). Families with breast cancer also had a propensity for multiple melanomas in females, suggesting that a sex-dependent factor may modify the phenotypic expression of CDKN2A alterations.

CONCLUSIONS: : Our findings confirm that the majority of CDKN2A-associated melanoma families in Sweden are due to a single founder mutation. They also show that families with the CDKN2A 113insArg mutation have an increased risk not only of multiple melanomas and pancreatic carcinoma but also of breast cancer.

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keywords
Amino Acid Sequence, Breast Neoplasms, Female, Genes, p16, Humans, Male, Melanoma, Molecular Sequence Data, Mutation, Neoplasms, Multiple Primary, Pancreatic Neoplasms, Pedigree, Polymerase Chain Reaction, Polymorphism, Single-Stranded Conformational, Promoter Regions, Genetic, Risk, Sex Factors, Skin Neoplasms, Sweden
in
Journal of the National Cancer Institute
volume
92
issue
15
pages
7 pages
publisher
Oxford University Press
external identifiers
  • Scopus:0034596343
ISSN
0027-8874
language
English
LU publication?
yes
id
d2f76c9a-1ec6-4cdc-9525-12366740c375
date added to LUP
2016-09-18 12:28:27
date last changed
2016-10-16 04:25:47
@misc{d2f76c9a-1ec6-4cdc-9525-12366740c375,
  abstract     = {<p>BACKGROUND: : Inherited mutations in the CDKN2A tumor suppressor gene, which encodes the p16(INK4a) protein, and in the cyclin-dependent kinase 4 (CDK4) gene confer susceptibility to cutaneous malignant melanoma. We analyzed families with two or more cases of melanoma for germline mutations in CDKN2A and CDK4 to elucidate the contribution of these gene defects to familial malignant melanoma and to the occurrence of other cancer types.</p><p>METHODS: : The entire CDKN2A coding region and exon 2 of the CDK4 gene of an affected member of each of 52 families from southern Sweden with at least two cases of melanoma in first- or second-degree relatives were screened for mutations by use of polymerase chain reaction-single-strand conformation polymorphism analysis. Statistical tests were two-sided.</p><p>RESULTS: : CDKN2A mutations were found in 10 (19%) of the 52 families. Nine families carried an identical alteration consisting of the insertion of arginine at position 113 of p16(INK4a), and one carried a missense mutation, in which the valine at position 115 was replaced with a glycine. The 113insArg mutant p16(INK4a) was unable to bind cdk4 and cdk6 in an in vitro binding assay. Six of the 113insArg families had at least one member with multiple primary melanomas; the 113insArg families also had a high frequency of other malignancies-in particular, breast cancer (a total of eight cases compared with the expected 2.1; P =.0014) and pancreatic cancer (a total of six cases compared with the expected 0.16; P&lt;.0001). Families with breast cancer also had a propensity for multiple melanomas in females, suggesting that a sex-dependent factor may modify the phenotypic expression of CDKN2A alterations.</p><p>CONCLUSIONS: : Our findings confirm that the majority of CDKN2A-associated melanoma families in Sweden are due to a single founder mutation. They also show that families with the CDKN2A 113insArg mutation have an increased risk not only of multiple melanomas and pancreatic carcinoma but also of breast cancer.</p>},
  author       = {Borg, Åke and Sandberg, T and Nilsson, K and Johannsson, O and Klinker, M and Måsbäck, A and Westerdahl, J and Olsson, Håkan and Ingvar, C},
  issn         = {0027-8874},
  keyword      = {Amino Acid Sequence,Breast Neoplasms,Female,Genes, p16,Humans,Male,Melanoma,Molecular Sequence Data,Mutation,Neoplasms, Multiple Primary,Pancreatic Neoplasms,Pedigree,Polymerase Chain Reaction,Polymorphism, Single-Stranded Conformational,Promoter Regions, Genetic,Risk,Sex Factors,Skin Neoplasms,Sweden},
  language     = {eng},
  month        = {08},
  number       = {15},
  pages        = {6--1260},
  publisher    = {ARRAY(0x8ccc130)},
  series       = {Journal of the National Cancer Institute},
  title        = {High frequency of multiple melanomas and breast and pancreas carcinomas in CDKN2A mutation-positive melanoma families},
  volume       = {92},
  year         = {2000},
}