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Platanetin and 7-iodo-acridone-4-carboxylic acid are not specific inhibitors of respiratory NAD(P)H dehydrogenases in potato tuber mitochondria

Roberts, Thomas H.; Rasmusson, Allan G. LU and Møller, Ian M. (1996) In Physiologia Plantarum 96(2). p.263-267
Abstract

Progress in understanding the role of NAD(P)H oxidation in plant respiration is restricted by the lack of access to specific inhibitors of each of the unknown number of NAD(P)H dehydrogenases in the inner mitochondrial membrane. Platanetin (3,5,7,8-tetrahydroxy-6-isoprenyl flavone) is known to be an inhibitor of external NADH oxidation by plant mitochondria, while 7-iodo-acridone-4-carboxylic acid (IACA) is an inhibitor of an internal, rotenone-insensitive NAD(P)H dehydrogenase isolated from yeast mitochondria. Here we show that platanetin inhibits external NAD(P)H oxidation by intact potato (Solanum tuberosum L. cv. Bintje) tuber mitochondria, deamino-NADH oxidation by Complex I assayed using inside-out submitochondrial particles from... (More)

Progress in understanding the role of NAD(P)H oxidation in plant respiration is restricted by the lack of access to specific inhibitors of each of the unknown number of NAD(P)H dehydrogenases in the inner mitochondrial membrane. Platanetin (3,5,7,8-tetrahydroxy-6-isoprenyl flavone) is known to be an inhibitor of external NADH oxidation by plant mitochondria, while 7-iodo-acridone-4-carboxylic acid (IACA) is an inhibitor of an internal, rotenone-insensitive NAD(P)H dehydrogenase isolated from yeast mitochondria. Here we show that platanetin inhibits external NAD(P)H oxidation by intact potato (Solanum tuberosum L. cv. Bintje) tuber mitochondria, deamino-NADH oxidation by Complex I assayed using inside-out submitochondrial particles from these mitochondria, and rotenone-insensitive NAD(P)H oxidation by these submitochondrial particles. IACA was found to inhibit the oxidation of external NADH and succinate by intact mitochondria with similar efficiency. However, IACA also inhibited NADPH and duroquinol oxidation by intact mitochondria as well as deamino-NADH and NAD(P)H oxidation by inside-out submitochondrial particles. This indicates that IACA has several sites of inhibition in the electron transport chain. The lack of specificity of both platanetin and IACA prevents these inhibitors from being used to shed more light on the identity of the NAD(P)H dehydrogenases in plant mitochondria.

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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
7-Iodo-acridone-4-carboxylic acid, Mitochondria, NAD(P)H dehydrogenases, Platanetin, Potato, Solanum tuberosum
in
Physiologia Plantarum
volume
96
issue
2
pages
5 pages
publisher
Wiley-Blackwell
external identifiers
  • Scopus:0030065916
ISSN
0031-9317
DOI
10.1111/j.1399-3054.1996.tb00212.x
language
English
LU publication?
no
id
ebd67020-a29e-4f77-8fc3-b4d84d4d61ce
date added to LUP
2016-05-31 21:31:16
date last changed
2016-08-10 14:34:04
@misc{ebd67020-a29e-4f77-8fc3-b4d84d4d61ce,
  abstract     = {<p>Progress in understanding the role of NAD(P)H oxidation in plant respiration is restricted by the lack of access to specific inhibitors of each of the unknown number of NAD(P)H dehydrogenases in the inner mitochondrial membrane. Platanetin (3,5,7,8-tetrahydroxy-6-isoprenyl flavone) is known to be an inhibitor of external NADH oxidation by plant mitochondria, while 7-iodo-acridone-4-carboxylic acid (IACA) is an inhibitor of an internal, rotenone-insensitive NAD(P)H dehydrogenase isolated from yeast mitochondria. Here we show that platanetin inhibits external NAD(P)H oxidation by intact potato (Solanum tuberosum L. cv. Bintje) tuber mitochondria, deamino-NADH oxidation by Complex I assayed using inside-out submitochondrial particles from these mitochondria, and rotenone-insensitive NAD(P)H oxidation by these submitochondrial particles. IACA was found to inhibit the oxidation of external NADH and succinate by intact mitochondria with similar efficiency. However, IACA also inhibited NADPH and duroquinol oxidation by intact mitochondria as well as deamino-NADH and NAD(P)H oxidation by inside-out submitochondrial particles. This indicates that IACA has several sites of inhibition in the electron transport chain. The lack of specificity of both platanetin and IACA prevents these inhibitors from being used to shed more light on the identity of the NAD(P)H dehydrogenases in plant mitochondria.</p>},
  author       = {Roberts, Thomas H. and Rasmusson, Allan G. and Møller, Ian M.},
  issn         = {0031-9317},
  keyword      = {7-Iodo-acridone-4-carboxylic acid,Mitochondria,NAD(P)H dehydrogenases,Platanetin,Potato,Solanum tuberosum},
  language     = {eng},
  number       = {2},
  pages        = {263--267},
  publisher    = {ARRAY(0x7e17d28)},
  series       = {Physiologia Plantarum},
  title        = {Platanetin and 7-iodo-acridone-4-carboxylic acid are not specific inhibitors of respiratory NAD(P)H dehydrogenases in potato tuber mitochondria},
  url          = {http://dx.doi.org/10.1111/j.1399-3054.1996.tb00212.x},
  volume       = {96},
  year         = {1996},
}