6-Hydroxydopamine induced degeneration of noradrenaline neurons in the scorbutic guinea-pig
(1974) In Biochemical Pharmacology 23(18). p.2585-2593- Abstract
The effect of the neurotoxic compound 6-hydroxydopamine and its immediate precursor 6-hydroxy-DOPA on noradrenaline uptake and storage in central and peripheral catecholamine neurons of scorbutic and normal guinea-pigs has been investigated. Endogenous noradrenaline in heart and brain as well as the in vitro uptake-accumulation of 3H-noradrenaline in iris and slices of heart and brain were not significantly changed in scorbutic animals. The in vitro formation of 3H-noradrenaline from 3H-dopamine was markedly reduced in heart slices of scorbutic guinea-pigs, due to ascorbic acid being a co-factor for dopamine-β-hydroxylase. There was an increased depletion of brain noradrenaline following tyrosine... (More)
The effect of the neurotoxic compound 6-hydroxydopamine and its immediate precursor 6-hydroxy-DOPA on noradrenaline uptake and storage in central and peripheral catecholamine neurons of scorbutic and normal guinea-pigs has been investigated. Endogenous noradrenaline in heart and brain as well as the in vitro uptake-accumulation of 3H-noradrenaline in iris and slices of heart and brain were not significantly changed in scorbutic animals. The in vitro formation of 3H-noradrenaline from 3H-dopamine was markedly reduced in heart slices of scorbutic guinea-pigs, due to ascorbic acid being a co-factor for dopamine-β-hydroxylase. There was an increased depletion of brain noradrenaline following tyrosine hydroxylase inhibition produced by α-methyl-p-tyrosine methylester in scorbutic animals, indicating an increased NA turnover. Administration of 6-hydroxydopamine or 6-hydroxy-DOPA resulted in a similar reduction of endogenous NA in brain and heart as well as of the in vitro uptake of 3H-noradrenaline in iris, and slices from heart, cerebral cortex and hypothalamus in scorbutic and control guinea-pigs. These results are discussed in view of current hypotheses on mechanisms involved in the neurotoxic action of 6-hydroxydopamine on catecholamine neurons.
(Less)
- author
- Jonsson, Gösta ; Lohmander, Stefan LU and Sachs, Charlotte
- organization
- publishing date
- 1974-09-15
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biochemical Pharmacology
- volume
- 23
- issue
- 18
- pages
- 9 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0016215994
- pmid:4153539
- ISSN
- 0006-2952
- DOI
- 10.1016/0006-2952(74)90181-6
- language
- English
- LU publication?
- yes
- id
- f01a0071-9e58-4950-ba28-3419ecac307c
- date added to LUP
- 2016-05-04 12:38:56
- date last changed
- 2024-01-04 02:37:31
@article{f01a0071-9e58-4950-ba28-3419ecac307c, abstract = {{<p>The effect of the neurotoxic compound 6-hydroxydopamine and its immediate precursor 6-hydroxy-DOPA on noradrenaline uptake and storage in central and peripheral catecholamine neurons of scorbutic and normal guinea-pigs has been investigated. Endogenous noradrenaline in heart and brain as well as the in vitro uptake-accumulation of <sup>3</sup>H-noradrenaline in iris and slices of heart and brain were not significantly changed in scorbutic animals. The in vitro formation of <sup>3</sup>H-noradrenaline from <sup>3</sup>H-dopamine was markedly reduced in heart slices of scorbutic guinea-pigs, due to ascorbic acid being a co-factor for dopamine-β-hydroxylase. There was an increased depletion of brain noradrenaline following tyrosine hydroxylase inhibition produced by α-methyl-p-tyrosine methylester in scorbutic animals, indicating an increased NA turnover. Administration of 6-hydroxydopamine or 6-hydroxy-DOPA resulted in a similar reduction of endogenous NA in brain and heart as well as of the in vitro uptake of <sup>3</sup>H-noradrenaline in iris, and slices from heart, cerebral cortex and hypothalamus in scorbutic and control guinea-pigs. These results are discussed in view of current hypotheses on mechanisms involved in the neurotoxic action of 6-hydroxydopamine on catecholamine neurons.</p>}}, author = {{Jonsson, Gösta and Lohmander, Stefan and Sachs, Charlotte}}, issn = {{0006-2952}}, language = {{eng}}, month = {{09}}, number = {{18}}, pages = {{2585--2593}}, publisher = {{Elsevier}}, series = {{Biochemical Pharmacology}}, title = {{6-Hydroxydopamine induced degeneration of noradrenaline neurons in the scorbutic guinea-pig}}, url = {{http://dx.doi.org/10.1016/0006-2952(74)90181-6}}, doi = {{10.1016/0006-2952(74)90181-6}}, volume = {{23}}, year = {{1974}}, }