Advanced

6-Hydroxydopamine induced degeneration of noradrenaline neurons in the scorbutic guinea-pig

Jonsson, Gösta; Lohmander, Stefan LU and Sachs, Charlotte (1974) In Biochemical Pharmacology 23(18). p.2585-2593
Abstract

The effect of the neurotoxic compound 6-hydroxydopamine and its immediate precursor 6-hydroxy-DOPA on noradrenaline uptake and storage in central and peripheral catecholamine neurons of scorbutic and normal guinea-pigs has been investigated. Endogenous noradrenaline in heart and brain as well as the in vitro uptake-accumulation of 3H-noradrenaline in iris and slices of heart and brain were not significantly changed in scorbutic animals. The in vitro formation of 3H-noradrenaline from 3H-dopamine was markedly reduced in heart slices of scorbutic guinea-pigs, due to ascorbic acid being a co-factor for dopamine-β-hydroxylase. There was an increased depletion of brain noradrenaline following tyrosine... (More)

The effect of the neurotoxic compound 6-hydroxydopamine and its immediate precursor 6-hydroxy-DOPA on noradrenaline uptake and storage in central and peripheral catecholamine neurons of scorbutic and normal guinea-pigs has been investigated. Endogenous noradrenaline in heart and brain as well as the in vitro uptake-accumulation of 3H-noradrenaline in iris and slices of heart and brain were not significantly changed in scorbutic animals. The in vitro formation of 3H-noradrenaline from 3H-dopamine was markedly reduced in heart slices of scorbutic guinea-pigs, due to ascorbic acid being a co-factor for dopamine-β-hydroxylase. There was an increased depletion of brain noradrenaline following tyrosine hydroxylase inhibition produced by α-methyl-p-tyrosine methylester in scorbutic animals, indicating an increased NA turnover. Administration of 6-hydroxydopamine or 6-hydroxy-DOPA resulted in a similar reduction of endogenous NA in brain and heart as well as of the in vitro uptake of 3H-noradrenaline in iris, and slices from heart, cerebral cortex and hypothalamus in scorbutic and control guinea-pigs. These results are discussed in view of current hypotheses on mechanisms involved in the neurotoxic action of 6-hydroxydopamine on catecholamine neurons. © 1974.

(Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biochemical Pharmacology
volume
23
issue
18
pages
9 pages
publisher
Elsevier
external identifiers
  • Scopus:0016215994
ISSN
0006-2952
DOI
10.1016/0006-2952(74)90181-6
language
English
LU publication?
yes
id
f01a0071-9e58-4950-ba28-3419ecac307c
date added to LUP
2016-05-04 12:38:56
date last changed
2016-11-14 09:42:49
@misc{f01a0071-9e58-4950-ba28-3419ecac307c,
  abstract     = {<p>The effect of the neurotoxic compound 6-hydroxydopamine and its immediate precursor 6-hydroxy-DOPA on noradrenaline uptake and storage in central and peripheral catecholamine neurons of scorbutic and normal guinea-pigs has been investigated. Endogenous noradrenaline in heart and brain as well as the in vitro uptake-accumulation of <sup>3</sup>H-noradrenaline in iris and slices of heart and brain were not significantly changed in scorbutic animals. The in vitro formation of <sup>3</sup>H-noradrenaline from <sup>3</sup>H-dopamine was markedly reduced in heart slices of scorbutic guinea-pigs, due to ascorbic acid being a co-factor for dopamine-β-hydroxylase. There was an increased depletion of brain noradrenaline following tyrosine hydroxylase inhibition produced by α-methyl-p-tyrosine methylester in scorbutic animals, indicating an increased NA turnover. Administration of 6-hydroxydopamine or 6-hydroxy-DOPA resulted in a similar reduction of endogenous NA in brain and heart as well as of the in vitro uptake of <sup>3</sup>H-noradrenaline in iris, and slices from heart, cerebral cortex and hypothalamus in scorbutic and control guinea-pigs. These results are discussed in view of current hypotheses on mechanisms involved in the neurotoxic action of 6-hydroxydopamine on catecholamine neurons. © 1974.</p>},
  author       = {Jonsson, Gösta and Lohmander, Stefan and Sachs, Charlotte},
  issn         = {0006-2952},
  language     = {eng},
  month        = {09},
  number       = {18},
  pages        = {2585--2593},
  publisher    = {ARRAY(0xb210ca8)},
  series       = {Biochemical Pharmacology},
  title        = {6-Hydroxydopamine induced degeneration of noradrenaline neurons in the scorbutic guinea-pig},
  url          = {http://dx.doi.org/10.1016/0006-2952(74)90181-6},
  volume       = {23},
  year         = {1974},
}