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Purification and crystallization of the I subunit of magnesium chelatase

Lv, You LU (2011) KEMT10 20111
Department of Chemistry
Abstract
The I subunit of magnesium chelatase is an AAA+ protein which binds and
hydrolyzes ATP to drive the insertion of Mg2+ into protoporphyrin IX during bio-synthesis of bacteriochlorophyll and chlorophyll. The purification method and co-crystallization conditions of the Rhodobacter capsulatus BchI (bacteriochlorophyll Magnesium Chelatase I subunit) protein with either ADP or AMPPNP and crystallization conditions of Synechocystissp sp 6803 ChlI (chlorophyll Magnesium Chelatase I subunit) protein are described. This is the first report of a successful preparation of the crystals of BchI-ADP complex, the crystals of BchI-AMPPNP complex and crystals of ChlI protein. The crystals of both proteins will be used for further structure determination.
Please use this url to cite or link to this publication:
author
Lv, You LU
supervisor
organization
course
KEMT10 20111
year
type
H2 - Master's Degree (Two Years)
subject
keywords
Magnesium chelatase, BchI, ChlI, AAA+ protein, Proteinvetenskap
language
English
id
3053630
date added to LUP
2012-09-19 11:48:11
date last changed
2012-09-19 11:48:11
@misc{3053630,
  abstract     = {{The I subunit of magnesium chelatase is an AAA+ protein which binds and
hydrolyzes ATP to drive the insertion of Mg2+ into protoporphyrin IX during bio-synthesis of bacteriochlorophyll and chlorophyll. The purification method and co-crystallization conditions of the Rhodobacter capsulatus BchI (bacteriochlorophyll Magnesium Chelatase I subunit) protein with either ADP or AMPPNP and crystallization conditions of Synechocystissp sp 6803 ChlI (chlorophyll Magnesium Chelatase I subunit) protein are described. This is the first report of a successful preparation of the crystals of BchI-ADP complex, the crystals of BchI-AMPPNP complex and crystals of ChlI protein. The crystals of both proteins will be used for further structure determination.}},
  author       = {{Lv, You}},
  language     = {{eng}},
  note         = {{Student Paper}},
  title        = {{Purification and crystallization of the I subunit of magnesium chelatase}},
  year         = {{2011}},
}