Studies on the mineral usage by bacterial pathogens
(2016) KEMP42 20161Department of Chemistry
- Abstract
- Cofactors are essential for the growth of bacterial pathogens also inside a human host and one source of cofactors are proteins in blood. Pathogens can use surface proteins to bind and extract cofactors from these blood proteins. Here, using protein modeling of these proteins in complex with bacterial protein combined with bacterial growth assays with blood proteins from different species, we demonstrate that a certain bacterium preferably utilizes the one of the peptide chains of a specific blood protein for its growth. We also mapped a putative binding region in this blood protein. Moreover, redox status of these proteins appears to affect the growth of this bacterium in a dose-response manner. Unexpectedly, the key bacterial surface... (More)
- Cofactors are essential for the growth of bacterial pathogens also inside a human host and one source of cofactors are proteins in blood. Pathogens can use surface proteins to bind and extract cofactors from these blood proteins. Here, using protein modeling of these proteins in complex with bacterial protein combined with bacterial growth assays with blood proteins from different species, we demonstrate that a certain bacterium preferably utilizes the one of the peptide chains of a specific blood protein for its growth. We also mapped a putative binding region in this blood protein. Moreover, redox status of these proteins appears to affect the growth of this bacterium in a dose-response manner. Unexpectedly, the key bacterial surface receptor is not essential for bacterial growth in presence of high concentrations of the blood protein. In conclusion, these results will be important in the design of novel therapies against bacterial pathogens. (Less)
Please use this url to cite or link to this publication:
http://lup.lub.lu.se/student-papers/record/8892457
- author
- Ortenlöf, Niklas LU
- supervisor
- organization
- course
- KEMP42 20161
- year
- 2016
- type
- L3 - Miscellaneous, Projetcs etc.
- subject
- keywords
- protein science, proteinvetenskap
- language
- English
- id
- 8892457
- date added to LUP
- 2017-07-13 11:24:43
- date last changed
- 2017-07-13 11:24:43
@misc{8892457, abstract = {{Cofactors are essential for the growth of bacterial pathogens also inside a human host and one source of cofactors are proteins in blood. Pathogens can use surface proteins to bind and extract cofactors from these blood proteins. Here, using protein modeling of these proteins in complex with bacterial protein combined with bacterial growth assays with blood proteins from different species, we demonstrate that a certain bacterium preferably utilizes the one of the peptide chains of a specific blood protein for its growth. We also mapped a putative binding region in this blood protein. Moreover, redox status of these proteins appears to affect the growth of this bacterium in a dose-response manner. Unexpectedly, the key bacterial surface receptor is not essential for bacterial growth in presence of high concentrations of the blood protein. In conclusion, these results will be important in the design of novel therapies against bacterial pathogens.}}, author = {{Ortenlöf, Niklas}}, language = {{eng}}, note = {{Student Paper}}, title = {{Studies on the mineral usage by bacterial pathogens}}, year = {{2016}}, }