Adsorption of lysozyme on Silica Surfaces and Specific ion effects
(2019) KEMP37 20182Department of Chemistry
- Abstract
- Biomedical application of nanoparticles is largely associated to their fate in biological media which, in fact, is related to their surface properties. Surface functionalitzation plays a key role in determining biodegradation, cytotoxicity and biodistribution through interactions which may be meditated by the macromolecules occurring in biological media. This project is focused on the effect of different buffers on lysozyme adsorption on flat silica surfaces and hydrophobized silica surfaces as well as mesoporous silica nanoparticles functionalized with amino groups (MSN-NH2). In order to know the adsorption amount of lysozyme we used ellipsometry, where each buffer has been studied independently at two different buffer concentrations at... (More)
- Biomedical application of nanoparticles is largely associated to their fate in biological media which, in fact, is related to their surface properties. Surface functionalitzation plays a key role in determining biodegradation, cytotoxicity and biodistribution through interactions which may be meditated by the macromolecules occurring in biological media. This project is focused on the effect of different buffers on lysozyme adsorption on flat silica surfaces and hydrophobized silica surfaces as well as mesoporous silica nanoparticles functionalized with amino groups (MSN-NH2). In order to know the adsorption amount of lysozyme we used ellipsometry, where each buffer has been studied independently at two different buffer concentrations at the same pH. The effect of different buffers can be related to specific ion effect described interaction of buffers and salts induces relevant effects on the charged interfaces (Hofmeister effect), and thus lysozyme loading.
Lysozyme adsorption on silica and hydrophobized silica as well mesoporous silica at pH 7.15 was found to be buffer specific. The BES buffer seems to present a unique characteristic behavior which does not allow desorption. TRIS buffer shows the highest lysozyme adsorption amounts, while citrate and phosphate buffer usually show quite similar results.
The sequential addition of MSN-NH2 causes more extensive desorption of lysozyme from the flat surface as observed with ellipsometry. Large effects of the buffer are also observed. The competitive adsorption to the particles, i. e. lysozyme protein corona formation, is likely to promote detachment from the silica surface (Less) - Popular Abstract
- The use of improved pharmaceutical formulations, which release the drug at the targeted cancerous tissue only, would reduce undesired side effects of the chemotherapeutics. Thus, nanoparticles are often helpful in order to reduce those side effects in biological media due to their surface properties. Surface functionalitzation plays a key role in in determining biodegradation, cytotoxicity and biodistribution through interactions which may be meditated by the macromolecules occurring in biological media.
This project is focused on the effect of different buffers on lysozyme adsorption on flat silica surfaces and hydrophobized silica surfaces as well as mesoporous silica nanoparticles functionalized with amino groups (MSN-NH2).
In order... (More) - The use of improved pharmaceutical formulations, which release the drug at the targeted cancerous tissue only, would reduce undesired side effects of the chemotherapeutics. Thus, nanoparticles are often helpful in order to reduce those side effects in biological media due to their surface properties. Surface functionalitzation plays a key role in in determining biodegradation, cytotoxicity and biodistribution through interactions which may be meditated by the macromolecules occurring in biological media.
This project is focused on the effect of different buffers on lysozyme adsorption on flat silica surfaces and hydrophobized silica surfaces as well as mesoporous silica nanoparticles functionalized with amino groups (MSN-NH2).
In order to know the adsorption amount of lysozyme we used ellipsometry, where each buffer has been studied independently at two different buffer concentrations at the same pH. The effect of different buffers can be related to specific ion effect described interaction of buffers and salts induces relevant effects on the charged interfaces (Hofmeister effect), and thus lysozyme loading.
Lysozyme adsorption on mesoporous silica at pH 7.15 was found to be buffer specific.
The sequential addition of MSN-NH2 causes more extensive desorption of lysozyme from the flat surface as observed with ellipsometry. Large effects of the buffer are also observed. The competitive adsorption to the particles, i. e. lysozyme protein corona formation, is likely to promote detachment from the silica surface.
Lysozyme adsorption relies upon buffer salts electrostatics considerations on MSNs surfaces; citrate and phosphate molecular structures grant an akind lysozyme loading performance while BES and TRIS present an utterly diverse performance.
Conclusevly, these nanoparticles would allow to conduct further studies on targeting cancerous cells as their surface properties could be used in biological media. (Less)
Please use this url to cite or link to this publication:
http://lup.lub.lu.se/student-papers/record/8984918
- author
- Onyos de Plandolit Ruiz, Guillem LU
- supervisor
- organization
- course
- KEMP37 20182
- year
- 2019
- type
- H2 - Master's Degree (Two Years)
- subject
- keywords
- Ellipsometry, mesoporous silica nanoparticles, surfaces, physical chemistry, fysikalisk kemi
- language
- English
- additional info
- Thesis
- id
- 8984918
- date added to LUP
- 2019-07-04 13:47:17
- date last changed
- 2019-07-04 13:47:17
@misc{8984918,
abstract = {{Biomedical application of nanoparticles is largely associated to their fate in biological media which, in fact, is related to their surface properties. Surface functionalitzation plays a key role in determining biodegradation, cytotoxicity and biodistribution through interactions which may be meditated by the macromolecules occurring in biological media. This project is focused on the effect of different buffers on lysozyme adsorption on flat silica surfaces and hydrophobized silica surfaces as well as mesoporous silica nanoparticles functionalized with amino groups (MSN-NH2). In order to know the adsorption amount of lysozyme we used ellipsometry, where each buffer has been studied independently at two different buffer concentrations at the same pH. The effect of different buffers can be related to specific ion effect described interaction of buffers and salts induces relevant effects on the charged interfaces (Hofmeister effect), and thus lysozyme loading.
Lysozyme adsorption on silica and hydrophobized silica as well mesoporous silica at pH 7.15 was found to be buffer specific. The BES buffer seems to present a unique characteristic behavior which does not allow desorption. TRIS buffer shows the highest lysozyme adsorption amounts, while citrate and phosphate buffer usually show quite similar results.
The sequential addition of MSN-NH2 causes more extensive desorption of lysozyme from the flat surface as observed with ellipsometry. Large effects of the buffer are also observed. The competitive adsorption to the particles, i. e. lysozyme protein corona formation, is likely to promote detachment from the silica surface}},
author = {{Onyos de Plandolit Ruiz, Guillem}},
language = {{eng}},
note = {{Student Paper}},
title = {{Adsorption of lysozyme on Silica Surfaces and Specific ion effects}},
year = {{2019}},
}