Synthesis of a 13C-labeled tool compound for diagnostic applications
(2020) KEMR10 20192Department of Chemistry
- Abstract
- Overconsumption and abuse of alcohol are common causes of disabilities and disease. In some cases, it can even lead to premature death. To detect alcohol abuse and overconsumption of alcohol, medical history, patient symptoms and different biomarkers can be used.
In this thesis the alcohol specific phosphatidylethanol (PEth) biomarker has been of interest. PEth is the collective name for a group of glycerolphospolipid homologues which are only created in the presence of ethanol in the body. To be able to detect and quantify the amount of PEth in a patient a reference molecule is needed. Through High Performance Liquid Chromatography (HPLC) a prepared blood sample, from the patient, and the reference molecule can be analysed. The... (More) - Overconsumption and abuse of alcohol are common causes of disabilities and disease. In some cases, it can even lead to premature death. To detect alcohol abuse and overconsumption of alcohol, medical history, patient symptoms and different biomarkers can be used.
In this thesis the alcohol specific phosphatidylethanol (PEth) biomarker has been of interest. PEth is the collective name for a group of glycerolphospolipid homologues which are only created in the presence of ethanol in the body. To be able to detect and quantify the amount of PEth in a patient a reference molecule is needed. Through High Performance Liquid Chromatography (HPLC) a prepared blood sample, from the patient, and the reference molecule can be analysed. The commercially available reference molecule is a deuterium labelled PEth 16:0/18:1. Since PEth 16:0/18:1 has been shown to be the most common PEth homologue in patients it is therefore used as a reference molecule.
Herein a new reference molecule to PEth with carbon-13 labelling has been suggested and synthesised. The synthesised carbon-13 PEth molecule has been evaluated at the university hospital in Lund (Anders Blomgren, Department of Clinical Chemistry, University Hospital of Lund), to compare if the new reference molecule is a better alternative than the commercially available one. (Less) - Popular Abstract
- Alcohol is a socially accepted drug in the western world and is something most of us encounter at social gatherings. Like most types of drugs, alcohol can lead to an addiction which has serious health problems and may lead to premature death. To be able to detect prolonged exposure to alcohol and alcohol abuse medical history, patient symptoms and biomarkers can be used.
Biomarkers are measurable indicators which can be found in the body and can be used in medicine to indicate a state of disease. When investigating a patient long term exposure to alcohol biomarkers are becoming more frequently used as they are very reliable. There is one biomarker which has been of special interest since it is specific to alcohol, it is only present in... (More) - Alcohol is a socially accepted drug in the western world and is something most of us encounter at social gatherings. Like most types of drugs, alcohol can lead to an addiction which has serious health problems and may lead to premature death. To be able to detect prolonged exposure to alcohol and alcohol abuse medical history, patient symptoms and biomarkers can be used.
Biomarkers are measurable indicators which can be found in the body and can be used in medicine to indicate a state of disease. When investigating a patient long term exposure to alcohol biomarkers are becoming more frequently used as they are very reliable. There is one biomarker which has been of special interest since it is specific to alcohol, it is only present in the body if the person has consumed alcohol, called Phosphatedylethanol or PEth for short. By analysing a patient blood sample and comparing it to a reference molecule, a compound similar to the biomarker, the amount of biomarker can be distinguished and a diagnosis of the patient can be made. As biomarkers are being used more frequently new reference molecules are needed which are reliable and accurate.
Herein a chemical synthesis of a new reference molecule for PEth, a carbon-13PEth reference, is described as well as improvements of the synthesis. The compound has been evaluated at the University hospital in Lund (Anders Blomgren, Department of Clinical Chemisty, University Hospital of Lund) to see if the carbon-13 labelled reference molecule is a more accurate reference molecule than the commercially available one. (Less)
Please use this url to cite or link to this publication:
http://lup.lub.lu.se/student-papers/record/9008193
- author
- Gummesson, Sofi LU
- supervisor
- organization
- course
- KEMR10 20192
- year
- 2020
- type
- H2 - Master's Degree (Two Years)
- subject
- keywords
- Organic chemistry, Organisk kemi
- language
- English
- id
- 9008193
- date added to LUP
- 2020-07-06 13:56:36
- date last changed
- 2020-07-06 13:56:36
@misc{9008193,
abstract = {{Overconsumption and abuse of alcohol are common causes of disabilities and disease. In some cases, it can even lead to premature death. To detect alcohol abuse and overconsumption of alcohol, medical history, patient symptoms and different biomarkers can be used.
In this thesis the alcohol specific phosphatidylethanol (PEth) biomarker has been of interest. PEth is the collective name for a group of glycerolphospolipid homologues which are only created in the presence of ethanol in the body. To be able to detect and quantify the amount of PEth in a patient a reference molecule is needed. Through High Performance Liquid Chromatography (HPLC) a prepared blood sample, from the patient, and the reference molecule can be analysed. The commercially available reference molecule is a deuterium labelled PEth 16:0/18:1. Since PEth 16:0/18:1 has been shown to be the most common PEth homologue in patients it is therefore used as a reference molecule.
Herein a new reference molecule to PEth with carbon-13 labelling has been suggested and synthesised. The synthesised carbon-13 PEth molecule has been evaluated at the university hospital in Lund (Anders Blomgren, Department of Clinical Chemistry, University Hospital of Lund), to compare if the new reference molecule is a better alternative than the commercially available one.}},
author = {{Gummesson, Sofi}},
language = {{eng}},
note = {{Student Paper}},
title = {{Synthesis of a 13C-labeled tool compound for diagnostic applications}},
year = {{2020}},
}