In vitro imaging of b-cells using fluorescent cubic bicontinuous liquid crystalline nanoparticles
(2016) In RSC Advances 6. p.62119-62127- Abstract
- To evaluate the potential of monoolein-based cubic bicontinuous liquid crystalline nanoparticles (cubosomes) as diagnostic tools in diabetes and pancreatic β-cell transplantation, fluorescent cubosomes were formulated, entrapping within the lipid bilayer a newly synthesized, potent, hydrophobic dye. Cryo-TEM, SAXS, and DLS were performed to assess the morphological and structural aspects of this formulation. Rat pancreatic β-cells (INS-1E) readily took up the cubosome nanoparticles, as revealed by in vitro internalization tests, and flow-cytometric analyses confirmed the persistence of the fluorescence for up to 24 hours. The cytotoxicity of cubosomes at various concentrations, evaluated with a real-time analysis of proliferation, revealed... (More)
- To evaluate the potential of monoolein-based cubic bicontinuous liquid crystalline nanoparticles (cubosomes) as diagnostic tools in diabetes and pancreatic β-cell transplantation, fluorescent cubosomes were formulated, entrapping within the lipid bilayer a newly synthesized, potent, hydrophobic dye. Cryo-TEM, SAXS, and DLS were performed to assess the morphological and structural aspects of this formulation. Rat pancreatic β-cells (INS-1E) readily took up the cubosome nanoparticles, as revealed by in vitro internalization tests, and flow-cytometric analyses confirmed the persistence of the fluorescence for up to 24 hours. The cytotoxicity of cubosomes at various concentrations, evaluated with a real-time analysis of proliferation, revealed that the proliferation curves relative to the less concentrated formulation (5 μM in terms of monoolein content) were comparable to the untreated cultures. INS-1E cell cycle and apoptosis supported such results. In conclusion, this formulation, with dye content in the nanomolar range, seems to be useful for β-cell imaging. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/02a02fbf-1668-4693-9e16-9985dd0cbbbb
- author
- organization
- publishing date
- 2016-06-23
- type
- Contribution to journal
- publication status
- published
- subject
- in
- RSC Advances
- volume
- 6
- pages
- 9 pages
- publisher
- Royal Society of Chemistry
- external identifiers
-
- scopus:84977274180
- ISSN
- 2046-2069
- DOI
- 10.1039/C6RA09616F
- language
- English
- LU publication?
- yes
- id
- 02a02fbf-1668-4693-9e16-9985dd0cbbbb
- date added to LUP
- 2016-07-20 15:39:18
- date last changed
- 2022-04-24 08:49:13
@article{02a02fbf-1668-4693-9e16-9985dd0cbbbb, abstract = {{To evaluate the potential of monoolein-based cubic bicontinuous liquid crystalline nanoparticles (cubosomes) as diagnostic tools in diabetes and pancreatic β-cell transplantation, fluorescent cubosomes were formulated, entrapping within the lipid bilayer a newly synthesized, potent, hydrophobic dye. Cryo-TEM, SAXS, and DLS were performed to assess the morphological and structural aspects of this formulation. Rat pancreatic β-cells (INS-1E) readily took up the cubosome nanoparticles, as revealed by in vitro internalization tests, and flow-cytometric analyses confirmed the persistence of the fluorescence for up to 24 hours. The cytotoxicity of cubosomes at various concentrations, evaluated with a real-time analysis of proliferation, revealed that the proliferation curves relative to the less concentrated formulation (5 μM in terms of monoolein content) were comparable to the untreated cultures. INS-1E cell cycle and apoptosis supported such results. In conclusion, this formulation, with dye content in the nanomolar range, seems to be useful for β-cell imaging.}}, author = {{Miceli, Vitale and Meli, Valeria and Blanchard-Desce, Mireille and Bsaibess, Talia and Pampalone, Mariangela and Conaldi, Pier Giulio and Caltagirone, Claudia and Obiols-Rabasa, Marc and Schmidt, Judith and Talmon, Yeshayahu and Casu, Anna and Murgia, Sergio}}, issn = {{2046-2069}}, language = {{eng}}, month = {{06}}, pages = {{62119--62127}}, publisher = {{Royal Society of Chemistry}}, series = {{RSC Advances}}, title = {{In vitro imaging of b-cells using fluorescent cubic bicontinuous liquid crystalline nanoparticles}}, url = {{http://dx.doi.org/10.1039/C6RA09616F}}, doi = {{10.1039/C6RA09616F}}, volume = {{6}}, year = {{2016}}, }