Enzyme-linked immunosorbent assay using thin-layered microfluidics with perfect capture of the target protein
(2023) In Analytical Methods 15(5). p.675-684- Abstract
We developed a process for enzyme-linked immunosorbent assay on a glass microchip via the use of a thin-layered microfluidic channel. This channel possesses a high aspect ratio (width/depth ∼200) and has an antibody layer immobilized directly on the channel surface. A depth of several microns and an excessive width and length (mm scale) of the channel provide a large-volume capacity (102 nL) and maximum capture efficiency of the analyte for a high level of detection sensitivity (102 pg mL−1). The developed reusable immunosensor has demonstrated high-performance characteristics by requiring less than 50 μL of sample and providing analysis in less than 25 min. This new method could impact the development... (More)
We developed a process for enzyme-linked immunosorbent assay on a glass microchip via the use of a thin-layered microfluidic channel. This channel possesses a high aspect ratio (width/depth ∼200) and has an antibody layer immobilized directly on the channel surface. A depth of several microns and an excessive width and length (mm scale) of the channel provide a large-volume capacity (102 nL) and maximum capture efficiency of the analyte for a high level of detection sensitivity (102 pg mL−1). The developed reusable immunosensor has demonstrated high-performance characteristics by requiring less than 50 μL of sample and providing analysis in less than 25 min. This new method could impact the development of point-of-care devices for biomedical applications.
(Less)
- author
- Smirnova, Adelina ; Ohta, Ryoichi ; Mori, Emi ; Shimizu, Hisashi ; Morikawa, Kyojiro and Kitamori, Takehiko LU
- organization
- publishing date
- 2023
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analytical Methods
- volume
- 15
- issue
- 5
- pages
- 10 pages
- publisher
- Royal Society of Chemistry
- external identifiers
-
- pmid:36655604
- scopus:85146905373
- ISSN
- 1759-9660
- DOI
- 10.1039/d2ay01686a
- language
- English
- LU publication?
- yes
- id
- 03cb858d-3650-49a9-9825-a60f8d539687
- date added to LUP
- 2023-02-14 15:15:00
- date last changed
- 2024-05-30 23:05:00
@article{03cb858d-3650-49a9-9825-a60f8d539687, abstract = {{<p>We developed a process for enzyme-linked immunosorbent assay on a glass microchip via the use of a thin-layered microfluidic channel. This channel possesses a high aspect ratio (width/depth ∼200) and has an antibody layer immobilized directly on the channel surface. A depth of several microns and an excessive width and length (mm scale) of the channel provide a large-volume capacity (10<sup>2</sup> nL) and maximum capture efficiency of the analyte for a high level of detection sensitivity (10<sup>2</sup> pg mL<sup>−1</sup>). The developed reusable immunosensor has demonstrated high-performance characteristics by requiring less than 50 μL of sample and providing analysis in less than 25 min. This new method could impact the development of point-of-care devices for biomedical applications.</p>}}, author = {{Smirnova, Adelina and Ohta, Ryoichi and Mori, Emi and Shimizu, Hisashi and Morikawa, Kyojiro and Kitamori, Takehiko}}, issn = {{1759-9660}}, language = {{eng}}, number = {{5}}, pages = {{675--684}}, publisher = {{Royal Society of Chemistry}}, series = {{Analytical Methods}}, title = {{Enzyme-linked immunosorbent assay using thin-layered microfluidics with perfect capture of the target protein}}, url = {{http://dx.doi.org/10.1039/d2ay01686a}}, doi = {{10.1039/d2ay01686a}}, volume = {{15}}, year = {{2023}}, }