Aeration rate effect on the growth kinetics, phytase production and plasmid stability of recombinant Escherichia coli BL21 (DE3)
(2014) In Journal of Pure and Applied Microbiology 8(4). p.2721-2728- Abstract
Phytase production using Escherichia coli BL21 (DE3), a recombinant strain harboring a plasmid encoding thermostable Bacillus phytase, in semi-industrial scale was studied in this work. Among the factors needed to be considered in order to achieve high enzyme yield, aeration rate plays an important role. Suitable aeration is required to supply cells with sufficient amount of air for cell and phytase production. The effect of different aeration rates, (1.0, 2.0 and 3.0 vv-1min-1), on the kinetics of cell growth and phytase production by the recombinant E. coli BL21 (DE3) in 16-L pilot scale stirred tank bioreactor was investigated. The highest cell concentration of 3.81 gL-1 concomitant with the maximal... (More)
Phytase production using Escherichia coli BL21 (DE3), a recombinant strain harboring a plasmid encoding thermostable Bacillus phytase, in semi-industrial scale was studied in this work. Among the factors needed to be considered in order to achieve high enzyme yield, aeration rate plays an important role. Suitable aeration is required to supply cells with sufficient amount of air for cell and phytase production. The effect of different aeration rates, (1.0, 2.0 and 3.0 vv-1min-1), on the kinetics of cell growth and phytase production by the recombinant E. coli BL21 (DE3) in 16-L pilot scale stirred tank bioreactor was investigated. The highest cell concentration of 3.81 gL-1 concomitant with the maximal total phytase production of 15.63 UmL-1 were obtained in a bioreactor of aeration rate 3.0vv-1min-1. At this high aeration rate, a maximal specific growth rate (μmax) and the maximal specific phytase production of 0.33 h-1 and 4102Ug-1, respectively, were achieved.
(Less)
- author
- Othman, Nor Zalina ; Elsayed, Elsayed A. ; Malek, Roslinda Abd ; Ramli, Solleh ; Masri, Helmy J. ; Sarmidi, Mohamed R. ; Aziz, Ramlan ; Wadaan, Mohamad A. ; Hatti-Kaul, Rajni LU and El Enshasy, Hesham A.
- organization
- publishing date
- 2014-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Aeration rate, Bioreactor cultivation, Escherichia coli, Phytase production, Plasmid stability
- in
- Journal of Pure and Applied Microbiology
- volume
- 8
- issue
- 4
- pages
- 8 pages
- publisher
- DR M N KHAN
- external identifiers
-
- scopus:84908201182
- ISSN
- 0973-7510
- language
- English
- LU publication?
- yes
- id
- 060444f7-54b0-460b-a2eb-6424505b8e35
- date added to LUP
- 2019-07-12 04:21:31
- date last changed
- 2022-03-25 22:12:21
@article{060444f7-54b0-460b-a2eb-6424505b8e35, abstract = {{<p>Phytase production using Escherichia coli BL21 (DE3), a recombinant strain harboring a plasmid encoding thermostable Bacillus phytase, in semi-industrial scale was studied in this work. Among the factors needed to be considered in order to achieve high enzyme yield, aeration rate plays an important role. Suitable aeration is required to supply cells with sufficient amount of air for cell and phytase production. The effect of different aeration rates, (1.0, 2.0 and 3.0 vv<sup>-1</sup>min<sup>-1</sup>), on the kinetics of cell growth and phytase production by the recombinant E. coli BL21 (DE3) in 16-L pilot scale stirred tank bioreactor was investigated. The highest cell concentration of 3.81 gL<sup>-1</sup> concomitant with the maximal total phytase production of 15.63 UmL<sup>-1</sup> were obtained in a bioreactor of aeration rate 3.0vv<sup>-1</sup>min<sup>-1</sup>. At this high aeration rate, a maximal specific growth rate (μ<sub>max</sub>) and the maximal specific phytase production of 0.33 h<sup>-1</sup> and 4102Ug<sup>-1</sup>, respectively, were achieved.</p>}}, author = {{Othman, Nor Zalina and Elsayed, Elsayed A. and Malek, Roslinda Abd and Ramli, Solleh and Masri, Helmy J. and Sarmidi, Mohamed R. and Aziz, Ramlan and Wadaan, Mohamad A. and Hatti-Kaul, Rajni and El Enshasy, Hesham A.}}, issn = {{0973-7510}}, keywords = {{Aeration rate; Bioreactor cultivation; Escherichia coli; Phytase production; Plasmid stability}}, language = {{eng}}, month = {{01}}, number = {{4}}, pages = {{2721--2728}}, publisher = {{DR M N KHAN}}, series = {{Journal of Pure and Applied Microbiology}}, title = {{Aeration rate effect on the growth kinetics, phytase production and plasmid stability of recombinant Escherichia coli BL21 (DE3)}}, volume = {{8}}, year = {{2014}}, }