Embedded Microbubbles for Acoustic Manipulation of Single Cells and Microfluidic Applications
(2021) In Analytical Chemistry 93(28). p.9760-9770- Abstract
Acoustically excited microstructures have demonstrated significant potential for small-scale biomedical applications by overcoming major microfluidic limitations. Recently, the application of oscillating microbubbles has demonstrated their superiority over acoustically excited solid structures due to their enhanced acoustic streaming at low input power. However, their limited temporal stability hinders their direct applicability for industrial or clinical purposes. Here, we introduce the embedded microbubble, a novel acoustofluidic design based on the combination of solid structures (poly(dimethylsiloxane)) and microbubbles (air-filled cavity) to combine the benefits of both approaches while minimizing their drawbacks. We investigate... (More)
Acoustically excited microstructures have demonstrated significant potential for small-scale biomedical applications by overcoming major microfluidic limitations. Recently, the application of oscillating microbubbles has demonstrated their superiority over acoustically excited solid structures due to their enhanced acoustic streaming at low input power. However, their limited temporal stability hinders their direct applicability for industrial or clinical purposes. Here, we introduce the embedded microbubble, a novel acoustofluidic design based on the combination of solid structures (poly(dimethylsiloxane)) and microbubbles (air-filled cavity) to combine the benefits of both approaches while minimizing their drawbacks. We investigate the influence of various design parameters and geometrical features through numerical simulations and experimentally evaluate their manipulation capabilities. Finally, we demonstrate the capabilities of our design for microfluidic applications by investigating its mixing performance as well as through the controlled rotational manipulation of individual HeLa cells.
(Less)
- author
- Läubli, Nino F ; Gerlt, Michael S LU ; Wüthrich, Alexander ; Lewis, Renard T M ; Shamsudhin, Naveen ; Kutay, Ulrike ; Ahmed, Daniel ; Dual, Jürg and Nelson, Bradley J
- publishing date
- 2021-07-20
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Acoustics, HeLa Cells, Humans, Microbubbles, Microfluidics
- in
- Analytical Chemistry
- volume
- 93
- issue
- 28
- pages
- 11 pages
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- scopus:85110940867
- pmid:34228921
- ISSN
- 1520-6882
- DOI
- 10.1021/acs.analchem.1c01209
- language
- English
- LU publication?
- no
- id
- 06f3ef60-5739-4499-a57e-99592379294a
- date added to LUP
- 2023-04-12 09:18:44
- date last changed
- 2024-07-13 04:13:37
@article{06f3ef60-5739-4499-a57e-99592379294a, abstract = {{<p>Acoustically excited microstructures have demonstrated significant potential for small-scale biomedical applications by overcoming major microfluidic limitations. Recently, the application of oscillating microbubbles has demonstrated their superiority over acoustically excited solid structures due to their enhanced acoustic streaming at low input power. However, their limited temporal stability hinders their direct applicability for industrial or clinical purposes. Here, we introduce the embedded microbubble, a novel acoustofluidic design based on the combination of solid structures (poly(dimethylsiloxane)) and microbubbles (air-filled cavity) to combine the benefits of both approaches while minimizing their drawbacks. We investigate the influence of various design parameters and geometrical features through numerical simulations and experimentally evaluate their manipulation capabilities. Finally, we demonstrate the capabilities of our design for microfluidic applications by investigating its mixing performance as well as through the controlled rotational manipulation of individual HeLa cells.</p>}}, author = {{Läubli, Nino F and Gerlt, Michael S and Wüthrich, Alexander and Lewis, Renard T M and Shamsudhin, Naveen and Kutay, Ulrike and Ahmed, Daniel and Dual, Jürg and Nelson, Bradley J}}, issn = {{1520-6882}}, keywords = {{Acoustics; HeLa Cells; Humans; Microbubbles; Microfluidics}}, language = {{eng}}, month = {{07}}, number = {{28}}, pages = {{9760--9770}}, publisher = {{The American Chemical Society (ACS)}}, series = {{Analytical Chemistry}}, title = {{Embedded Microbubbles for Acoustic Manipulation of Single Cells and Microfluidic Applications}}, url = {{http://dx.doi.org/10.1021/acs.analchem.1c01209}}, doi = {{10.1021/acs.analchem.1c01209}}, volume = {{93}}, year = {{2021}}, }