LETM proteins play a role in the accumulation of mitochondrially encoded proteins in Arabidopsis thaliana and AtLETM2 displays parent of origin effects
(2012) In Journal of Biological Chemistry 287(50). p.73-41757- Abstract
The Arabidopsis thaliana genome contains two genes with homology to the mitochondrial protein LETM1 (leucine zipper-EF-hand-containing transmembrane protein). Inactivation of both genes, Atletm1 and Atletm2, together is lethal. Plants that are hemizygous for AtLETM2 and homozygous for Atletm1 (letm1(-/-) LETM2(+/-)) displayed a mild retarded growth phenotype during early seedling growth. It was shown that accumulation of mitochondrial proteins was reduced in hemizygous (letm1(-/-) LETM2(+/-)) plants. Examination of respiratory chain proteins by Western blotting, blue native PAGE, and enzymatic activity assays revealed that the steady state level of ATP synthase was reduced in abundance, whereas the steady state levels of other... (More)
The Arabidopsis thaliana genome contains two genes with homology to the mitochondrial protein LETM1 (leucine zipper-EF-hand-containing transmembrane protein). Inactivation of both genes, Atletm1 and Atletm2, together is lethal. Plants that are hemizygous for AtLETM2 and homozygous for Atletm1 (letm1(-/-) LETM2(+/-)) displayed a mild retarded growth phenotype during early seedling growth. It was shown that accumulation of mitochondrial proteins was reduced in hemizygous (letm1(-/-) LETM2(+/-)) plants. Examination of respiratory chain proteins by Western blotting, blue native PAGE, and enzymatic activity assays revealed that the steady state level of ATP synthase was reduced in abundance, whereas the steady state levels of other respiratory chain proteins remained unchanged. The absence of a functional maternal AtLETM2 allele in an Atletm1 mutant background resulted in early seed abortion. Reciprocal crosses revealed that maternally, but not paternally, derived AtLETM2 was absolutely required for seed development. This requirement for a functional maternal allele of AtLETM2 was confirmed using direct sequencing of reciprocal crosses of Col-0 and Ler accessions. Furthermore, AtLETM2 promoter β-glucuronidase constructs displayed exclusive maternal expression patterns.
(Less)
- author
- publishing date
- 2012-12-07
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Arabidopsis, Arabidopsis Proteins, Electron Transport, Gene Expression Regulation, Plant, Mitochondria, Mitochondrial Proteins
- in
- Journal of Biological Chemistry
- volume
- 287
- issue
- 50
- pages
- 17 pages
- publisher
- American Society for Biochemistry and Molecular Biology
- external identifiers
-
- scopus:84871311489
- pmid:23043101
- ISSN
- 1083-351X
- DOI
- 10.1074/jbc.M112.383836
- language
- English
- LU publication?
- no
- id
- 06faf07a-1528-4c03-9491-8ea46508891b
- date added to LUP
- 2017-05-09 10:06:51
- date last changed
- 2024-10-15 05:46:49
@article{06faf07a-1528-4c03-9491-8ea46508891b, abstract = {{<p>The Arabidopsis thaliana genome contains two genes with homology to the mitochondrial protein LETM1 (leucine zipper-EF-hand-containing transmembrane protein). Inactivation of both genes, Atletm1 and Atletm2, together is lethal. Plants that are hemizygous for AtLETM2 and homozygous for Atletm1 (letm1(-/-) LETM2(+/-)) displayed a mild retarded growth phenotype during early seedling growth. It was shown that accumulation of mitochondrial proteins was reduced in hemizygous (letm1(-/-) LETM2(+/-)) plants. Examination of respiratory chain proteins by Western blotting, blue native PAGE, and enzymatic activity assays revealed that the steady state level of ATP synthase was reduced in abundance, whereas the steady state levels of other respiratory chain proteins remained unchanged. The absence of a functional maternal AtLETM2 allele in an Atletm1 mutant background resulted in early seed abortion. Reciprocal crosses revealed that maternally, but not paternally, derived AtLETM2 was absolutely required for seed development. This requirement for a functional maternal allele of AtLETM2 was confirmed using direct sequencing of reciprocal crosses of Col-0 and Ler accessions. Furthermore, AtLETM2 promoter β-glucuronidase constructs displayed exclusive maternal expression patterns.</p>}}, author = {{Zhang, Botao and Carrie, Chris and Ivanova, Aneta and Narsai, Reena and Murcha, Monika W and Duncan, Owen and Wang, Yan and Law, Simon R and Albrecht, Verónica and Pogson, Barry James and Giraud, Estelle and Van Aken, Olivier and Whelan, James}}, issn = {{1083-351X}}, keywords = {{Arabidopsis; Arabidopsis Proteins; Electron Transport; Gene Expression Regulation, Plant; Mitochondria; Mitochondrial Proteins}}, language = {{eng}}, month = {{12}}, number = {{50}}, pages = {{73--41757}}, publisher = {{American Society for Biochemistry and Molecular Biology}}, series = {{Journal of Biological Chemistry}}, title = {{LETM proteins play a role in the accumulation of mitochondrially encoded proteins in Arabidopsis thaliana and AtLETM2 displays parent of origin effects}}, url = {{http://dx.doi.org/10.1074/jbc.M112.383836}}, doi = {{10.1074/jbc.M112.383836}}, volume = {{287}}, year = {{2012}}, }