Plasmodium falciparum : a simplified technique for obtaining singly infected erythrocytes
(2005) In Parasitology Research 95(3). p.8-176- Abstract
We report the development of a simple technique involving 15 ml polypropylene tubes and a rotatory incubator for obtaining erythrocytes singly infected with Plasmodium falciparum. This technique will be useful for cloning of the parasite. Our finding that P. falciparum merozoite invasion is inhibited during rotation suggests that this method may also be useful for the study of parasite-erythrocyte interactions under dynamic circulatory conditions.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/0ed015ae-d5ed-4b79-84b9-516a4957bc7b
- author
- Puthia, Manoj K LU and Tan, Kevin S W
- publishing date
- 2005-02
- type
- Contribution to journal
- publication status
- published
- keywords
- Animals, Culture Media, Erythrocytes/cytology, Humans, Malaria, Falciparum/parasitology, Parasitemia/parasitology, Parasitology/instrumentation, Plasmodium falciparum/growth & development, Polypropylenes
- in
- Parasitology Research
- volume
- 95
- issue
- 3
- pages
- 8 - 176
- publisher
- Springer
- external identifiers
-
- pmid:15616858
- scopus:15044345186
- ISSN
- 0932-0113
- DOI
- 10.1007/s00436-004-1266-6
- language
- English
- LU publication?
- no
- id
- 0ed015ae-d5ed-4b79-84b9-516a4957bc7b
- date added to LUP
- 2018-08-27 13:49:58
- date last changed
- 2024-01-15 00:23:20
@article{0ed015ae-d5ed-4b79-84b9-516a4957bc7b, abstract = {{<p>We report the development of a simple technique involving 15 ml polypropylene tubes and a rotatory incubator for obtaining erythrocytes singly infected with Plasmodium falciparum. This technique will be useful for cloning of the parasite. Our finding that P. falciparum merozoite invasion is inhibited during rotation suggests that this method may also be useful for the study of parasite-erythrocyte interactions under dynamic circulatory conditions.</p>}}, author = {{Puthia, Manoj K and Tan, Kevin S W}}, issn = {{0932-0113}}, keywords = {{Animals; Culture Media; Erythrocytes/cytology; Humans; Malaria, Falciparum/parasitology; Parasitemia/parasitology; Parasitology/instrumentation; Plasmodium falciparum/growth & development; Polypropylenes}}, language = {{eng}}, number = {{3}}, pages = {{8--176}}, publisher = {{Springer}}, series = {{Parasitology Research}}, title = {{Plasmodium falciparum : a simplified technique for obtaining singly infected erythrocytes}}, url = {{http://dx.doi.org/10.1007/s00436-004-1266-6}}, doi = {{10.1007/s00436-004-1266-6}}, volume = {{95}}, year = {{2005}}, }