Genomic organization of profilin-III and evidence for a transcript expressed exclusively in testis.
(2002) In Gene 283(1-2). p.219-225- Abstract
- Profilins are small, widely expressed actin binding proteins, thought to be key regulators of actin dynamics in living cells. So far, three profilin-genes have been described: profilin-I (PFN1), profilin-II (PFN2) with two splice variants and the recently identified profilin-III (PFN3). Here we describe the genomic organization of the genes encoding human and mouse profilin-III. Both are single exon genes and lie in close vicinity to the renal sodium-phosphate transport gene 2 (SLC34A1, NPT2) which is highly expressed in kidney. Northern hybridization to rat tissues has previously demonstrated expression of an approximately 4.5 kb long profilin-III mRNA transcript in kidney and a mRNA transcript of approximately 1 kb in length in testis.... (More)
- Profilins are small, widely expressed actin binding proteins, thought to be key regulators of actin dynamics in living cells. So far, three profilin-genes have been described: profilin-I (PFN1), profilin-II (PFN2) with two splice variants and the recently identified profilin-III (PFN3). Here we describe the genomic organization of the genes encoding human and mouse profilin-III. Both are single exon genes and lie in close vicinity to the renal sodium-phosphate transport gene 2 (SLC34A1, NPT2) which is highly expressed in kidney. Northern hybridization to rat tissues has previously demonstrated expression of an approximately 4.5 kb long profilin-III mRNA transcript in kidney and a mRNA transcript of approximately 1 kb in length in testis. Here we show that mouse profilin-III expression is restricted to testis and that the 4.2 kb profilin-III mRNA in kidney is the result of a slc34a1 transcript which includes the antisense profilin-III open reading frame in its 3prime prime or minute-untranslated region. Finally, we demonstrate by in situ hybridization that profilin-III mRNA is localized to cells in the late stage of spermatogenesis. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/106719
- author
- Braun, Attila LU ; Aszodi, Attila LU ; Hellebrand, Heide ; Berna, Alejandro ; Fässler, Reinhard LU and Brandau, Oliver LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- In Situ Hybridization, Human, Gene Expression, Kidney : metabolism, Mice, Male, Mice Inbred Strains, Microfilament Proteins : genetics, Molecular Sequence Data, Protein Isoforms : genetics, RNA Messenger : genetics : metabolism, Rats, Reverse Transcriptase Polymerase Chain Reaction, Sequence Alignment, Sequence Homology Amino Acid, Sequence Analysis DNA, Transcription Genetic, Testis : metabolism, DNA : chemistry : genetics, DNA Complementary : chemistry : genetics, Blotting Northern, Base Sequence, Animal, Amino Acid Sequence
- in
- Gene
- volume
- 283
- issue
- 1-2
- pages
- 219 - 225
- publisher
- Elsevier
- external identifiers
-
- wos:000174393400022
- pmid:11867228
- scopus:0037160530
- ISSN
- 1879-0038
- DOI
- 10.1016/S0378-1119(01)00855-1
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Pathology, (Lund) (013030000)
- id
- d0f07697-45a5-419e-b477-a5952aab214f (old id 106719)
- alternative location
- http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11867228&dopt=Abstract
- date added to LUP
- 2016-04-01 15:31:27
- date last changed
- 2022-04-22 08:09:13
@article{d0f07697-45a5-419e-b477-a5952aab214f, abstract = {{Profilins are small, widely expressed actin binding proteins, thought to be key regulators of actin dynamics in living cells. So far, three profilin-genes have been described: profilin-I (PFN1), profilin-II (PFN2) with two splice variants and the recently identified profilin-III (PFN3). Here we describe the genomic organization of the genes encoding human and mouse profilin-III. Both are single exon genes and lie in close vicinity to the renal sodium-phosphate transport gene 2 (SLC34A1, NPT2) which is highly expressed in kidney. Northern hybridization to rat tissues has previously demonstrated expression of an approximately 4.5 kb long profilin-III mRNA transcript in kidney and a mRNA transcript of approximately 1 kb in length in testis. Here we show that mouse profilin-III expression is restricted to testis and that the 4.2 kb profilin-III mRNA in kidney is the result of a slc34a1 transcript which includes the antisense profilin-III open reading frame in its 3prime prime or minute-untranslated region. Finally, we demonstrate by in situ hybridization that profilin-III mRNA is localized to cells in the late stage of spermatogenesis.}}, author = {{Braun, Attila and Aszodi, Attila and Hellebrand, Heide and Berna, Alejandro and Fässler, Reinhard and Brandau, Oliver}}, issn = {{1879-0038}}, keywords = {{In Situ Hybridization; Human; Gene Expression; Kidney : metabolism; Mice; Male; Mice Inbred Strains; Microfilament Proteins : genetics; Molecular Sequence Data; Protein Isoforms : genetics; RNA Messenger : genetics : metabolism; Rats; Reverse Transcriptase Polymerase Chain Reaction; Sequence Alignment; Sequence Homology Amino Acid; Sequence Analysis DNA; Transcription Genetic; Testis : metabolism; DNA : chemistry : genetics; DNA Complementary : chemistry : genetics; Blotting Northern; Base Sequence; Animal; Amino Acid Sequence}}, language = {{eng}}, number = {{1-2}}, pages = {{219--225}}, publisher = {{Elsevier}}, series = {{Gene}}, title = {{Genomic organization of profilin-III and evidence for a transcript expressed exclusively in testis.}}, url = {{http://dx.doi.org/10.1016/S0378-1119(01)00855-1}}, doi = {{10.1016/S0378-1119(01)00855-1}}, volume = {{283}}, year = {{2002}}, }