Hormone-sensitive lipase is not required for cholesteryl ester hydrolysis in macrophages.
(2002) In Biochemical and Biophysical Research Communications 292(4). p.900-903- Abstract
- Storage of cholesteryl esters in the cytoplasm of macrophages is one of the earliest and most ubiquitous event observed in the development of arteriosclerosis. Macrophages have an enormous capacity to uptake and store cholesterol in the form of cytosolic cholesteryl ester droplets. These stores are mobilized by the action of a neutral cholesteryl ester hydrolase (NCEH), producing free cholesterol that is either secreted to extracellular acceptors or reesterified. It has been proposed that hormone-sensitive lipase (HSL) is responsible for the NCEH activity in macrophages. The present work shows, however, that peritoneal macrophages from HSL null mice hydrolyze cytosolic stores of cholesteryl esters at a comparable rate to that of peritoneal... (More)
- Storage of cholesteryl esters in the cytoplasm of macrophages is one of the earliest and most ubiquitous event observed in the development of arteriosclerosis. Macrophages have an enormous capacity to uptake and store cholesterol in the form of cytosolic cholesteryl ester droplets. These stores are mobilized by the action of a neutral cholesteryl ester hydrolase (NCEH), producing free cholesterol that is either secreted to extracellular acceptors or reesterified. It has been proposed that hormone-sensitive lipase (HSL) is responsible for the NCEH activity in macrophages. The present work shows, however, that peritoneal macrophages from HSL null mice hydrolyze cytosolic stores of cholesteryl esters at a comparable rate to that of peritoneal macrophages from wild-type mice, therefore demonstrating that HSL is not the main NCEH in macrophages. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/107621
- author
- Contreras, Juan Antonio LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cholesterol Esterase : metabolism, Cholesterol Esterase : genetics, Cholesterol Esterase : deficiency, Cultured, Cells, Bucladesine : pharmacology, Western, Blotting, Arteriosclerosis : metabolism, Animal, Adipose Tissue : enzymology, Peritoneal : metabolism, Mice, Inbred Strains, Knockout, Cholesterol Esters : metabolism, Cytoplasm : metabolism, Hydrolysis, Macrophages, Peritoneal : drug effects
- in
- Biochemical and Biophysical Research Communications
- volume
- 292
- issue
- 4
- pages
- 900 - 903
- publisher
- Elsevier
- external identifiers
-
- pmid:11944899
- wos:000175171800018
- scopus:0036297998
- ISSN
- 1090-2104
- DOI
- 10.1006/bbrc.2002.6757
- language
- English
- LU publication?
- yes
- id
- d492982a-bf71-49c3-acaa-81f5bbaf578d (old id 107621)
- alternative location
- http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11944899&dopt=Abstract
- date added to LUP
- 2016-04-01 16:56:11
- date last changed
- 2022-01-28 23:12:03
@article{d492982a-bf71-49c3-acaa-81f5bbaf578d, abstract = {{Storage of cholesteryl esters in the cytoplasm of macrophages is one of the earliest and most ubiquitous event observed in the development of arteriosclerosis. Macrophages have an enormous capacity to uptake and store cholesterol in the form of cytosolic cholesteryl ester droplets. These stores are mobilized by the action of a neutral cholesteryl ester hydrolase (NCEH), producing free cholesterol that is either secreted to extracellular acceptors or reesterified. It has been proposed that hormone-sensitive lipase (HSL) is responsible for the NCEH activity in macrophages. The present work shows, however, that peritoneal macrophages from HSL null mice hydrolyze cytosolic stores of cholesteryl esters at a comparable rate to that of peritoneal macrophages from wild-type mice, therefore demonstrating that HSL is not the main NCEH in macrophages.}}, author = {{Contreras, Juan Antonio}}, issn = {{1090-2104}}, keywords = {{Cholesterol Esterase : metabolism; Cholesterol Esterase : genetics; Cholesterol Esterase : deficiency; Cultured; Cells; Bucladesine : pharmacology; Western; Blotting; Arteriosclerosis : metabolism; Animal; Adipose Tissue : enzymology; Peritoneal : metabolism; Mice; Inbred Strains; Knockout; Cholesterol Esters : metabolism; Cytoplasm : metabolism; Hydrolysis; Macrophages; Peritoneal : drug effects}}, language = {{eng}}, number = {{4}}, pages = {{900--903}}, publisher = {{Elsevier}}, series = {{Biochemical and Biophysical Research Communications}}, title = {{Hormone-sensitive lipase is not required for cholesteryl ester hydrolysis in macrophages.}}, url = {{http://dx.doi.org/10.1006/bbrc.2002.6757}}, doi = {{10.1006/bbrc.2002.6757}}, volume = {{292}}, year = {{2002}}, }