Primed B cells present type-II collagen to T cells.
(2002) In Scandinavian Journal of Immunology 55(4). p.382-389- Abstract
- Development of type-II collagen (CII)-induced arthritis (CIA) is dependent on a T-cell mediated activation of autoreactive B cells. However, it is still unclear if B cells can present CII to T cells. To investigate the role of B cells as antigen-presenting cells (APCs) for CII, we purified B cells from lymph nodes of immunized and nonimmunized mice. These B cells were used as APC for antigen-specific T-cell hybridomas. B cells from naïve mice did present native, triple-helical, CII (nCII) but also ovalbumin (OVA) and denatured CII (dCII) to antigen-specific T-cell hybridomas. In addition, B cells primed with nCII or OVA, but not dCII, activated the antigen-specific T-cell hybridomas two to three times better than naïve B cells. We conclude... (More)
- Development of type-II collagen (CII)-induced arthritis (CIA) is dependent on a T-cell mediated activation of autoreactive B cells. However, it is still unclear if B cells can present CII to T cells. To investigate the role of B cells as antigen-presenting cells (APCs) for CII, we purified B cells from lymph nodes of immunized and nonimmunized mice. These B cells were used as APC for antigen-specific T-cell hybridomas. B cells from naïve mice did present native, triple-helical, CII (nCII) but also ovalbumin (OVA) and denatured CII (dCII) to antigen-specific T-cell hybridomas. In addition, B cells primed with nCII or OVA, but not dCII, activated the antigen-specific T-cell hybridomas two to three times better than naïve B cells. We conclude that antigen-primed B cells have the capacity to process and present CII to primed T cells, and antigen-primed antigen-specific B cells are more efficient as APC than naïve B cells. We further conclude that B cells have the potential to play an important role as APC in the development of CIA. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/107767
- author
- Holmdahl, Meirav LU ; Vestberg, Mikael LU and Holmdahl, Rikard LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- B-Lymphocytes : physiology, Collagen Type II : immunology, Macrophages : physiology, Mice, Inbred C57BL, Support, Inbred DBA, Non-U.S. Gov't, T-Lymphocytes : immunology, Arthritis : etiology, Antigen-Presenting Cells : physiology, Antibodies : immunology, Animal
- in
- Scandinavian Journal of Immunology
- volume
- 55
- issue
- 4
- pages
- 382 - 389
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000175115000011
- pmid:11967120
- scopus:0036224790
- ISSN
- 1365-3083
- DOI
- 10.1046/j.1365-3083.2002.01071.x
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Medical Inflammation Research (013212019), Department of Dermatology and Venereology (Lund) (013006000)
- id
- 9a5c9918-f28d-42f7-9e4a-e617d47af020 (old id 107767)
- date added to LUP
- 2016-04-01 15:29:07
- date last changed
- 2022-01-28 05:32:42
@article{9a5c9918-f28d-42f7-9e4a-e617d47af020,
abstract = {{Development of type-II collagen (CII)-induced arthritis (CIA) is dependent on a T-cell mediated activation of autoreactive B cells. However, it is still unclear if B cells can present CII to T cells. To investigate the role of B cells as antigen-presenting cells (APCs) for CII, we purified B cells from lymph nodes of immunized and nonimmunized mice. These B cells were used as APC for antigen-specific T-cell hybridomas. B cells from naïve mice did present native, triple-helical, CII (nCII) but also ovalbumin (OVA) and denatured CII (dCII) to antigen-specific T-cell hybridomas. In addition, B cells primed with nCII or OVA, but not dCII, activated the antigen-specific T-cell hybridomas two to three times better than naïve B cells. We conclude that antigen-primed B cells have the capacity to process and present CII to primed T cells, and antigen-primed antigen-specific B cells are more efficient as APC than naïve B cells. We further conclude that B cells have the potential to play an important role as APC in the development of CIA.}},
author = {{Holmdahl, Meirav and Vestberg, Mikael and Holmdahl, Rikard}},
issn = {{1365-3083}},
keywords = {{B-Lymphocytes : physiology; Collagen Type II : immunology; Macrophages : physiology; Mice; Inbred C57BL; Support; Inbred DBA; Non-U.S. Gov't; T-Lymphocytes : immunology; Arthritis : etiology; Antigen-Presenting Cells : physiology; Antibodies : immunology; Animal}},
language = {{eng}},
number = {{4}},
pages = {{382--389}},
publisher = {{Wiley-Blackwell}},
series = {{Scandinavian Journal of Immunology}},
title = {{Primed B cells present type-II collagen to T cells.}},
url = {{https://lup.lub.lu.se/search/files/4402462/623602.pdf}},
doi = {{10.1046/j.1365-3083.2002.01071.x}},
volume = {{55}},
year = {{2002}},
}