Evaluation of four qualitative methods for detection of beta-lactamase production in Staphylococcus and Micrococcus species
(1989) In European Journal of Clinical Microbiology & Infectious Diseases 8(11). p.962-967- Abstract
- Four qualitative methods for the detection of beta-lactamase production in Staphylococcus and Micrococcus species were evaluated and compared with a quantitative macroiodometric reference method. The disc diffusion test with penicillin G and the cloverleaf method could not separate beta-lactamase-positive from beta-lactamase-negative strains. Two applications of the chromogenic cephalosporin test, using uninduced strains and strains grown on blood agar plates, gave a large number of false negative and false positive results. False negative reactions were most common among uninduced strains, while the false positive reactions were most often recorded for Staphylococcus saprophyticus. A high degree of efficiency was recorded for the... (More)
- Four qualitative methods for the detection of beta-lactamase production in Staphylococcus and Micrococcus species were evaluated and compared with a quantitative macroiodometric reference method. The disc diffusion test with penicillin G and the cloverleaf method could not separate beta-lactamase-positive from beta-lactamase-negative strains. Two applications of the chromogenic cephalosporin test, using uninduced strains and strains grown on blood agar plates, gave a large number of false negative and false positive results. False negative reactions were most common among uninduced strains, while the false positive reactions were most often recorded for Staphylococcus saprophyticus. A high degree of efficiency was recorded for the nitrocefin spot test, using induced strains grown on antibiotic susceptibility agar, and for the starch-iodine plate method. The starch-iodine plate with methicillin as inducer gave the most reliable results. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1104598
- author
- Petersson, A C ; Eliasson, Ingvar LU ; Kamme, C and Miörner, Håkan LU
- organization
- publishing date
- 1989
- type
- Contribution to journal
- publication status
- published
- subject
- in
- European Journal of Clinical Microbiology & Infectious Diseases
- volume
- 8
- issue
- 11
- pages
- 962 - 967
- publisher
- Springer
- external identifiers
-
- pmid:2513196
- scopus:0024351539
- ISSN
- 1435-4373
- DOI
- 10.1007/BF01967566
- language
- English
- LU publication?
- yes
- id
- 7fb96577-46ba-4de6-8791-54c72ea71182 (old id 1104598)
- date added to LUP
- 2016-04-01 16:56:49
- date last changed
- 2021-06-20 04:03:50
@article{7fb96577-46ba-4de6-8791-54c72ea71182, abstract = {{Four qualitative methods for the detection of beta-lactamase production in Staphylococcus and Micrococcus species were evaluated and compared with a quantitative macroiodometric reference method. The disc diffusion test with penicillin G and the cloverleaf method could not separate beta-lactamase-positive from beta-lactamase-negative strains. Two applications of the chromogenic cephalosporin test, using uninduced strains and strains grown on blood agar plates, gave a large number of false negative and false positive results. False negative reactions were most common among uninduced strains, while the false positive reactions were most often recorded for Staphylococcus saprophyticus. A high degree of efficiency was recorded for the nitrocefin spot test, using induced strains grown on antibiotic susceptibility agar, and for the starch-iodine plate method. The starch-iodine plate with methicillin as inducer gave the most reliable results.}}, author = {{Petersson, A C and Eliasson, Ingvar and Kamme, C and Miörner, Håkan}}, issn = {{1435-4373}}, language = {{eng}}, number = {{11}}, pages = {{962--967}}, publisher = {{Springer}}, series = {{European Journal of Clinical Microbiology & Infectious Diseases}}, title = {{Evaluation of four qualitative methods for detection of beta-lactamase production in Staphylococcus and Micrococcus species}}, url = {{http://dx.doi.org/10.1007/BF01967566}}, doi = {{10.1007/BF01967566}}, volume = {{8}}, year = {{1989}}, }