G proteins coupled to phospholipase C: molecular targets of long-term ethanol exposure
(1991) In Journal of Neurochemistry 56(6). p.2018-2026- Abstract
- Long-term ethanol exposure is known to inhibit bradykinin-stimulated phosphoinositide hydrolysis in cultures of neuroblastoma x glioma 108-15 cells. In the present study, [3H]bradykinin binding, GTP-binding protein function, and phospholipase C activity were assayed in cells grown for 4 days in 100 mM ethanol with the aim of elucidating the molecular target of ethanol on signal transduction coupled to inositol trisphosphate and diacylglycerol formation. Ethanol exposure reduced guanosine 5'-O-(3-thiotriphosphate) [GTP(S)]- and, to a lesser extent, NaF/AlCl3-stimulated phosphoinositide hydrolysis, whereas it had no effect on the enzymatic activity of a phosphatidylinositol 4,5-bisphosphate-specific phospholipase C. [3H]Bradykinin binding in... (More)
- Long-term ethanol exposure is known to inhibit bradykinin-stimulated phosphoinositide hydrolysis in cultures of neuroblastoma x glioma 108-15 cells. In the present study, [3H]bradykinin binding, GTP-binding protein function, and phospholipase C activity were assayed in cells grown for 4 days in 100 mM ethanol with the aim of elucidating the molecular target of ethanol on signal transduction coupled to inositol trisphosphate and diacylglycerol formation. Ethanol exposure reduced guanosine 5'-O-(3-thiotriphosphate) [GTP(S)]- and, to a lesser extent, NaF/AlCl3-stimulated phosphoinositide hydrolysis, whereas it had no effect on the enzymatic activity of a phosphatidylinositol 4,5-bisphosphate-specific phospholipase C. [3H]Bradykinin binding in the absence of GTP(S) was not influenced by ethanol exposure. However, the reduction in [3H]bradykinin binding seen in control cells after addition of GTP analogue was inhibited in cells grown in ethanol-containing medium. The results indicate that long-term ethanol exposure exerts its effects on receptor-stimulated phosphoinositide hydrolysis primarily at the level of the GTP-binding protein. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1105752
- author
- Simonsson, Per LU ; Rodriguez, F David ; Loman, Niklas LU and Alling, Christer LU
- organization
- publishing date
- 1991
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Bradykinin, Ethanol, Phosphatidylinositol 4, 5-bisphosphate, Phospholipase C, GTP-binding protein, NG 108–15 cell line
- in
- Journal of Neurochemistry
- volume
- 56
- issue
- 6
- pages
- 2018 - 2026
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:1851210
- scopus:0025944450
- ISSN
- 1471-4159
- DOI
- 10.1111/j.1471-4159.1991.tb03461.x
- language
- English
- LU publication?
- yes
- id
- 06b806dd-59bd-46f9-ac53-91501d5aa476 (old id 1105752)
- date added to LUP
- 2016-04-01 16:16:20
- date last changed
- 2021-01-03 11:20:28
@article{06b806dd-59bd-46f9-ac53-91501d5aa476, abstract = {{Long-term ethanol exposure is known to inhibit bradykinin-stimulated phosphoinositide hydrolysis in cultures of neuroblastoma x glioma 108-15 cells. In the present study, [3H]bradykinin binding, GTP-binding protein function, and phospholipase C activity were assayed in cells grown for 4 days in 100 mM ethanol with the aim of elucidating the molecular target of ethanol on signal transduction coupled to inositol trisphosphate and diacylglycerol formation. Ethanol exposure reduced guanosine 5'-O-(3-thiotriphosphate) [GTP(S)]- and, to a lesser extent, NaF/AlCl3-stimulated phosphoinositide hydrolysis, whereas it had no effect on the enzymatic activity of a phosphatidylinositol 4,5-bisphosphate-specific phospholipase C. [3H]Bradykinin binding in the absence of GTP(S) was not influenced by ethanol exposure. However, the reduction in [3H]bradykinin binding seen in control cells after addition of GTP analogue was inhibited in cells grown in ethanol-containing medium. The results indicate that long-term ethanol exposure exerts its effects on receptor-stimulated phosphoinositide hydrolysis primarily at the level of the GTP-binding protein.}}, author = {{Simonsson, Per and Rodriguez, F David and Loman, Niklas and Alling, Christer}}, issn = {{1471-4159}}, keywords = {{Bradykinin; Ethanol; Phosphatidylinositol 4; 5-bisphosphate; Phospholipase C; GTP-binding protein; NG 108–15 cell line}}, language = {{eng}}, number = {{6}}, pages = {{2018--2026}}, publisher = {{Wiley-Blackwell}}, series = {{Journal of Neurochemistry}}, title = {{G proteins coupled to phospholipase C: molecular targets of long-term ethanol exposure}}, url = {{http://dx.doi.org/10.1111/j.1471-4159.1991.tb03461.x}}, doi = {{10.1111/j.1471-4159.1991.tb03461.x}}, volume = {{56}}, year = {{1991}}, }