Ruthenium red and capsaicin induce a neurogenic inflammatory response in the rabbit eye: effects of omega-conotoxin GVIA and tetrodotoxin
(1991) In European Journal of Pharmacology 209(3). p.175-183- Abstract
- The effects of ruthenium red, an inorganic dye with known capsaicin antagonist properties, was investigated in the rabbit eye. At a dose of 0.24 nmol ruthenium red inhibited the inflammatory effects of capsaicin (1 or 8 nmol). Unexpectedly, when the dye was injected in doses ranging from 0.24 to 7.4 nmol, it caused an inflammatory response with constriction of the pupil (miosis) and a breakdown of the blood-aqueous barrier, leading to a rise intraocular pressure. Tetrodotoxin (30 nmol) inhibited the ruthenium red-induced rise in intraocular pressure but had less effect on the miotic response. The tachykinin antagonist spantide inhibited the miosis but had no effect on the rise in intraocular pressure. Ruthenium red induced an increase in... (More)
- The effects of ruthenium red, an inorganic dye with known capsaicin antagonist properties, was investigated in the rabbit eye. At a dose of 0.24 nmol ruthenium red inhibited the inflammatory effects of capsaicin (1 or 8 nmol). Unexpectedly, when the dye was injected in doses ranging from 0.24 to 7.4 nmol, it caused an inflammatory response with constriction of the pupil (miosis) and a breakdown of the blood-aqueous barrier, leading to a rise intraocular pressure. Tetrodotoxin (30 nmol) inhibited the ruthenium red-induced rise in intraocular pressure but had less effect on the miotic response. The tachykinin antagonist spantide inhibited the miosis but had no effect on the rise in intraocular pressure. Ruthenium red induced an increase in substance P-like immunoreactivity and calcitonin gene-related peptide-like immunoreactivity in the aqueous humor. These levels were positively correlated with the rise in aqueous humor protein concentration. The ruthenium red-induced miosis and, to a less extent, the rise in intraocular pressure were inhibited by the Ca2+ channel-blocking agent omega-conotoxin GVIA (CTX), indicating a partial dependence on an influx of extracellular Ca2+. CTX also attenuated the miotic effect of capsaicin but had no effect on the capsaicin-induced rise in intraocular pressure. It is concluded that, in the rabbit eye, ruthenium red induces a neurogenic inflammatory response besides its capsaicin antagonist effects. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1106139
- author
- Andersson, Sven LU and Le Greves, Pierre
- publishing date
- 1991
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Blood-aqueous barrier, CGRP (calcitonin gene-related peptide), Capsaicin, Miosis, Ruthenium red, Substance P
- in
- European Journal of Pharmacology
- volume
- 209
- issue
- 3
- pages
- 175 - 183
- publisher
- Elsevier
- external identifiers
-
- pmid:1724655
- scopus:0025840387
- ISSN
- 1879-0712
- DOI
- 10.1016/0014-2999(91)90167-O
- language
- English
- LU publication?
- no
- id
- f5ff862a-7f74-45df-8b97-dbe8d66af7e7 (old id 1106139)
- date added to LUP
- 2016-04-01 12:16:49
- date last changed
- 2021-01-03 07:42:42
@article{f5ff862a-7f74-45df-8b97-dbe8d66af7e7,
abstract = {{The effects of ruthenium red, an inorganic dye with known capsaicin antagonist properties, was investigated in the rabbit eye. At a dose of 0.24 nmol ruthenium red inhibited the inflammatory effects of capsaicin (1 or 8 nmol). Unexpectedly, when the dye was injected in doses ranging from 0.24 to 7.4 nmol, it caused an inflammatory response with constriction of the pupil (miosis) and a breakdown of the blood-aqueous barrier, leading to a rise intraocular pressure. Tetrodotoxin (30 nmol) inhibited the ruthenium red-induced rise in intraocular pressure but had less effect on the miotic response. The tachykinin antagonist spantide inhibited the miosis but had no effect on the rise in intraocular pressure. Ruthenium red induced an increase in substance P-like immunoreactivity and calcitonin gene-related peptide-like immunoreactivity in the aqueous humor. These levels were positively correlated with the rise in aqueous humor protein concentration. The ruthenium red-induced miosis and, to a less extent, the rise in intraocular pressure were inhibited by the Ca2+ channel-blocking agent omega-conotoxin GVIA (CTX), indicating a partial dependence on an influx of extracellular Ca2+. CTX also attenuated the miotic effect of capsaicin but had no effect on the capsaicin-induced rise in intraocular pressure. It is concluded that, in the rabbit eye, ruthenium red induces a neurogenic inflammatory response besides its capsaicin antagonist effects.}},
author = {{Andersson, Sven and Le Greves, Pierre}},
issn = {{1879-0712}},
keywords = {{Blood-aqueous barrier; CGRP (calcitonin gene-related peptide); Capsaicin; Miosis; Ruthenium red; Substance P}},
language = {{eng}},
number = {{3}},
pages = {{175--183}},
publisher = {{Elsevier}},
series = {{European Journal of Pharmacology}},
title = {{Ruthenium red and capsaicin induce a neurogenic inflammatory response in the rabbit eye: effects of omega-conotoxin GVIA and tetrodotoxin}},
url = {{http://dx.doi.org/10.1016/0014-2999(91)90167-O}},
doi = {{10.1016/0014-2999(91)90167-O}},
volume = {{209}},
year = {{1991}},
}