Molecular cloning and expression of human myocardial cGMP-inhibited cAMP phosphodiesterase
(1992) In Proceedings of the National Academy of Sciences 89(9). p.3721-3725- Abstract
- We have cloned a cDNA for a myocardial cGMP-inhibited cAMP phosphodiesterase (cGI PDE) from a human heart cDNA library in lambda Zap II. The open reading frame [3.5 kilobases (kb)] of cDNA clone n.13.2 (7.7 kb) encodes a protein of 125 kDa. In Northern blots of total human ventricle RNA, a single mRNA species (8.3 kb) hybridized with a 4-kb EcoRI restriction fragment of clone n.13.2 cDNA (containing the entire open reading frame). The carboxyl-terminal region of the deduced amino acid sequence of the cGI PDE contains the putative catalytic domain conserved among mammalian PDE families. A partial cDNA clone, n.2, encoding a truncated, 54-kDa cGI PDE containing the conserved domain was expressed as a catalytically active fusion protein in... (More)
- We have cloned a cDNA for a myocardial cGMP-inhibited cAMP phosphodiesterase (cGI PDE) from a human heart cDNA library in lambda Zap II. The open reading frame [3.5 kilobases (kb)] of cDNA clone n.13.2 (7.7 kb) encodes a protein of 125 kDa. In Northern blots of total human ventricle RNA, a single mRNA species (8.3 kb) hybridized with a 4-kb EcoRI restriction fragment of clone n.13.2 cDNA (containing the entire open reading frame). The carboxyl-terminal region of the deduced amino acid sequence of the cGI PDE contains the putative catalytic domain conserved among mammalian PDE families. A partial cDNA clone, n.2, encoding a truncated, 54-kDa cGI PDE containing the conserved domain was expressed as a catalytically active fusion protein in Escherichia coli. cAMP hydrolytic activity was inhibited by cGMP and OPC 3911 but not by rolipram. Thus, this report provides direct proof that the conserved domain contains the catalytic core of cGI PDEs. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1106737
- author
- Meacci, E ; Taira, M ; Moos, M Jr ; Smith, C J ; Movsesian, M A ; Degerman, Eva LU ; Belfrage, P and Manganiello, V
- organization
- publishing date
- 1992
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Proceedings of the National Academy of Sciences
- volume
- 89
- issue
- 9
- pages
- 3721 - 3725
- publisher
- National Academy of Sciences
- external identifiers
-
- pmid:1315035
- scopus:0026552359
- ISSN
- 1091-6490
- language
- English
- LU publication?
- yes
- id
- 4f84779e-ed45-485d-bd51-6db3df5761bd (old id 1106737)
- date added to LUP
- 2016-04-01 11:59:20
- date last changed
- 2021-10-10 04:05:24
@article{4f84779e-ed45-485d-bd51-6db3df5761bd, abstract = {{We have cloned a cDNA for a myocardial cGMP-inhibited cAMP phosphodiesterase (cGI PDE) from a human heart cDNA library in lambda Zap II. The open reading frame [3.5 kilobases (kb)] of cDNA clone n.13.2 (7.7 kb) encodes a protein of 125 kDa. In Northern blots of total human ventricle RNA, a single mRNA species (8.3 kb) hybridized with a 4-kb EcoRI restriction fragment of clone n.13.2 cDNA (containing the entire open reading frame). The carboxyl-terminal region of the deduced amino acid sequence of the cGI PDE contains the putative catalytic domain conserved among mammalian PDE families. A partial cDNA clone, n.2, encoding a truncated, 54-kDa cGI PDE containing the conserved domain was expressed as a catalytically active fusion protein in Escherichia coli. cAMP hydrolytic activity was inhibited by cGMP and OPC 3911 but not by rolipram. Thus, this report provides direct proof that the conserved domain contains the catalytic core of cGI PDEs.}}, author = {{Meacci, E and Taira, M and Moos, M Jr and Smith, C J and Movsesian, M A and Degerman, Eva and Belfrage, P and Manganiello, V}}, issn = {{1091-6490}}, language = {{eng}}, number = {{9}}, pages = {{3721--3725}}, publisher = {{National Academy of Sciences}}, series = {{Proceedings of the National Academy of Sciences}}, title = {{Molecular cloning and expression of human myocardial cGMP-inhibited cAMP phosphodiesterase}}, volume = {{89}}, year = {{1992}}, }