Mechanisms of adaptation to the effects of ethanol on activation of phospholipase C in NG 108-15 cells
(1992) In Neuropharmacology 31(11). p.1157-1164- Abstract
- In this study the effect of different times of exposure to ethanol (1-7 days, 100 mM) on bradykinin and GTP(S)-stimulated activation of phospholipase C in NG 108-15 cells and on the binding of [3H]bradykinin to its receptors was investigated. Ethanol attenuated both agonist and GTP-analogue-induced hydrolysis of phosphoinositides for a period of up to 4 days of treatment, while exerting no effect on binding to bradykinin receptors. However, after 7 days of exposure to ethanol, the agonist-induced activation of phospholipase C was completely resistant to the inhibitory effects of alcohol. This finding correlated to a change in the affinity of the bradykinin receptor population after 7 days of treatment. The results indicate that... (More)
- In this study the effect of different times of exposure to ethanol (1-7 days, 100 mM) on bradykinin and GTP(S)-stimulated activation of phospholipase C in NG 108-15 cells and on the binding of [3H]bradykinin to its receptors was investigated. Ethanol attenuated both agonist and GTP-analogue-induced hydrolysis of phosphoinositides for a period of up to 4 days of treatment, while exerting no effect on binding to bradykinin receptors. However, after 7 days of exposure to ethanol, the agonist-induced activation of phospholipase C was completely resistant to the inhibitory effects of alcohol. This finding correlated to a change in the affinity of the bradykinin receptor population after 7 days of treatment. The results indicate that bradykinin-induced breakdown of phosphatidylinositol 4,5-bisphosphate adapts to the effects of ethanol, after long-term treatment. Possible adaptative changes taking place at the level of the G protein(s), may induce a shift in the affinity of the receptor population and, consequently, serve as a compensatory mechanism to counteract the inhibitory effect of ethanol. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1106764
- author
- Rodriguez, F D ; Simonsson, Per LU ; Gustavsson, L and Alling, Christer LU
- organization
- publishing date
- 1992
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- NG 108-15 cell line, adaptation, inositol phosphates, bradykinin, G proteins, ethanol
- in
- Neuropharmacology
- volume
- 31
- issue
- 11
- pages
- 1157 - 1164
- publisher
- Elsevier
- external identifiers
-
- pmid:1475023
- scopus:0026494830
- ISSN
- 1873-7064
- DOI
- 10.1016/0028-3908(92)90012-E
- language
- English
- LU publication?
- yes
- id
- d7757200-0541-4647-9372-4045c249409b (old id 1106764)
- date added to LUP
- 2016-04-01 12:19:42
- date last changed
- 2021-01-03 09:49:45
@article{d7757200-0541-4647-9372-4045c249409b,
abstract = {{In this study the effect of different times of exposure to ethanol (1-7 days, 100 mM) on bradykinin and GTP(S)-stimulated activation of phospholipase C in NG 108-15 cells and on the binding of [3H]bradykinin to its receptors was investigated. Ethanol attenuated both agonist and GTP-analogue-induced hydrolysis of phosphoinositides for a period of up to 4 days of treatment, while exerting no effect on binding to bradykinin receptors. However, after 7 days of exposure to ethanol, the agonist-induced activation of phospholipase C was completely resistant to the inhibitory effects of alcohol. This finding correlated to a change in the affinity of the bradykinin receptor population after 7 days of treatment. The results indicate that bradykinin-induced breakdown of phosphatidylinositol 4,5-bisphosphate adapts to the effects of ethanol, after long-term treatment. Possible adaptative changes taking place at the level of the G protein(s), may induce a shift in the affinity of the receptor population and, consequently, serve as a compensatory mechanism to counteract the inhibitory effect of ethanol.}},
author = {{Rodriguez, F D and Simonsson, Per and Gustavsson, L and Alling, Christer}},
issn = {{1873-7064}},
keywords = {{NG 108-15 cell line; adaptation; inositol phosphates; bradykinin; G proteins; ethanol}},
language = {{eng}},
number = {{11}},
pages = {{1157--1164}},
publisher = {{Elsevier}},
series = {{Neuropharmacology}},
title = {{Mechanisms of adaptation to the effects of ethanol on activation of phospholipase C in NG 108-15 cells}},
url = {{http://dx.doi.org/10.1016/0028-3908(92)90012-E}},
doi = {{10.1016/0028-3908(92)90012-E}},
volume = {{31}},
year = {{1992}},
}