Early B-Cell Factor, E2A, and Pax-5 Cooperate To Activate the Early B Cell-Specific mb-1 Promoter.
(2002) In Molecular and Cellular Biology 22(24). p.8539-8551- Abstract
- Previous studies have suggested that the early-B-cell-specific mb-1(Ig{alpha}) promoter is regulated by EBF and Pax-5. Here, we used in vivo footprinting assays to detect occupation of binding sites in endogenous mb-1 promoters at various stages of B-cell differentiation. In addition to EBF and Pax-5 binding sites, we detected occupancy of a consensus binding site for E2A proteins (E box) in pre-B cells. EBF and E box sites are crucial for promoter function in transfected pre-B cells, and EBF and E2A proteins synergistically activated the promoter in transfected HeLa cells. Other data suggest that EBF and E box sites are less important for promoter function at later stages of differentiation, whereas binding sites for Pax-5 (and its Ets... (More)
- Previous studies have suggested that the early-B-cell-specific mb-1(Ig{alpha}) promoter is regulated by EBF and Pax-5. Here, we used in vivo footprinting assays to detect occupation of binding sites in endogenous mb-1 promoters at various stages of B-cell differentiation. In addition to EBF and Pax-5 binding sites, we detected occupancy of a consensus binding site for E2A proteins (E box) in pre-B cells. EBF and E box sites are crucial for promoter function in transfected pre-B cells, and EBF and E2A proteins synergistically activated the promoter in transfected HeLa cells. Other data suggest that EBF and E box sites are less important for promoter function at later stages of differentiation, whereas binding sites for Pax-5 (and its Ets ternary complex partners) are required for promoter function in all mb-1-expressing cells. Using DNA microarrays, we found that expression of endogenous mb-1 transcripts correlates most closely with EBF expression and negatively with Id1, an inhibitor of E2A protein function, further linking regulation of the mb-1 gene with EBF and E2A. Together, our studies demonstrate the complexity of factors regulating tissue-specific transcription and support the concept that EBF, E2A, and Pax-5 cooperate to activate target genes in early B-cell development. (Less)
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https://lup.lub.lu.se/record/110995
- author
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Molecular and Cellular Biology
- volume
- 22
- issue
- 24
- pages
- 8539 - 8551
- publisher
- American Society for Microbiology
- external identifiers
-
- wos:000179527400015
- scopus:18744388827
- ISSN
- 0270-7306
- DOI
- 10.1128/MCB.22.24.8539-8551.2002
- language
- English
- LU publication?
- yes
- id
- ed504acb-3db1-44b2-bf75-600d26aa2d00 (old id 110995)
- date added to LUP
- 2016-04-01 12:13:36
- date last changed
- 2022-07-29 23:51:07
@article{ed504acb-3db1-44b2-bf75-600d26aa2d00, abstract = {{Previous studies have suggested that the early-B-cell-specific mb-1(Ig{alpha}) promoter is regulated by EBF and Pax-5. Here, we used in vivo footprinting assays to detect occupation of binding sites in endogenous mb-1 promoters at various stages of B-cell differentiation. In addition to EBF and Pax-5 binding sites, we detected occupancy of a consensus binding site for E2A proteins (E box) in pre-B cells. EBF and E box sites are crucial for promoter function in transfected pre-B cells, and EBF and E2A proteins synergistically activated the promoter in transfected HeLa cells. Other data suggest that EBF and E box sites are less important for promoter function at later stages of differentiation, whereas binding sites for Pax-5 (and its Ets ternary complex partners) are required for promoter function in all mb-1-expressing cells. Using DNA microarrays, we found that expression of endogenous mb-1 transcripts correlates most closely with EBF expression and negatively with Id1, an inhibitor of E2A protein function, further linking regulation of the mb-1 gene with EBF and E2A. Together, our studies demonstrate the complexity of factors regulating tissue-specific transcription and support the concept that EBF, E2A, and Pax-5 cooperate to activate target genes in early B-cell development.}}, author = {{Sigvardsson, Mikael and Clark, Dawn R and Fitzsimmons, Daniel and Doyle, Michelle and Åkerblad, Peter and Breslin, Thomas and Bilke, Sven and Li, Ronggui and Yeamans, Carmen and Zhang, Gongyi and Hagman, James}}, issn = {{0270-7306}}, language = {{eng}}, number = {{24}}, pages = {{8539--8551}}, publisher = {{American Society for Microbiology}}, series = {{Molecular and Cellular Biology}}, title = {{Early B-Cell Factor, E2A, and Pax-5 Cooperate To Activate the Early B Cell-Specific mb-1 Promoter.}}, url = {{https://lup.lub.lu.se/search/files/2835451/623673.pdf}}, doi = {{10.1128/MCB.22.24.8539-8551.2002}}, volume = {{22}}, year = {{2002}}, }