Extracorporeal immunoadsorption from whole blood based on the avidin-biotin concept. Evaluation of a new method
(1996) In Acta Oncologica 35(3). p.309-312- Abstract
- This study of 36 rats with rat colon adenocarcinoma transplants was carried out to investigate the efficacy of a new method of whole blood immunoadsorption (WBIA) in removing biotinylated monoclonal antibodies (MAbs) directly from unseparated blood, in order to increase 'the tumor/normal-tissue uptake ratio', as compared with extracorporeal immunoadsorption (ECIA) of antibodies from plasma. Compared with the ECIA system, the overall volume of the WBIA system (comprising only a pump, an adsorption column, a drop-chamber and tubings) was less (3.6 vs. 6.2 ml), and procedure duration 2 h less. The 17 rats undergoing the WBIA procedure, started 12 h after i.v. injection of 4.0-4.5 MBq 125I-BR96-biotin, manifested neither hemolysis nor any... (More)
- This study of 36 rats with rat colon adenocarcinoma transplants was carried out to investigate the efficacy of a new method of whole blood immunoadsorption (WBIA) in removing biotinylated monoclonal antibodies (MAbs) directly from unseparated blood, in order to increase 'the tumor/normal-tissue uptake ratio', as compared with extracorporeal immunoadsorption (ECIA) of antibodies from plasma. Compared with the ECIA system, the overall volume of the WBIA system (comprising only a pump, an adsorption column, a drop-chamber and tubings) was less (3.6 vs. 6.2 ml), and procedure duration 2 h less. The 17 rats undergoing the WBIA procedure, started 12 h after i.v. injection of 4.0-4.5 MBq 125I-BR96-biotin, manifested neither hemolysis nor any other complication; no signs of organ edema were found at dissection; whole body and blood radioactivity values were reduced by 51% and 89.5%, respectively. The WBIA method was as effective as ECIA, but technically simpler, safer and more reliable. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1110285
- author
- Garkavij, Michael LU ; Tennvall, Jan LU ; Strand, Sven-Erik LU ; Nilsson, Rune LU ; Lindgren, Lars ; Chen, Jangin ; Isaksson, Martin ; Eriksson, Håkan and Sjögren, Hans-Olof
- organization
- publishing date
- 1996
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Acta Oncologica
- volume
- 35
- issue
- 3
- pages
- 309 - 312
- publisher
- Taylor & Francis
- external identifiers
-
- pmid:8679261
- scopus:0029894616
- ISSN
- 1651-226X
- DOI
- 10.3109/02841869609101646
- language
- English
- LU publication?
- yes
- id
- 10c55935-bd9c-4c15-a855-ac5c41dcf476 (old id 1110285)
- date added to LUP
- 2016-04-01 16:04:36
- date last changed
- 2022-01-28 17:06:20
@article{10c55935-bd9c-4c15-a855-ac5c41dcf476, abstract = {{This study of 36 rats with rat colon adenocarcinoma transplants was carried out to investigate the efficacy of a new method of whole blood immunoadsorption (WBIA) in removing biotinylated monoclonal antibodies (MAbs) directly from unseparated blood, in order to increase 'the tumor/normal-tissue uptake ratio', as compared with extracorporeal immunoadsorption (ECIA) of antibodies from plasma. Compared with the ECIA system, the overall volume of the WBIA system (comprising only a pump, an adsorption column, a drop-chamber and tubings) was less (3.6 vs. 6.2 ml), and procedure duration 2 h less. The 17 rats undergoing the WBIA procedure, started 12 h after i.v. injection of 4.0-4.5 MBq 125I-BR96-biotin, manifested neither hemolysis nor any other complication; no signs of organ edema were found at dissection; whole body and blood radioactivity values were reduced by 51% and 89.5%, respectively. The WBIA method was as effective as ECIA, but technically simpler, safer and more reliable.}}, author = {{Garkavij, Michael and Tennvall, Jan and Strand, Sven-Erik and Nilsson, Rune and Lindgren, Lars and Chen, Jangin and Isaksson, Martin and Eriksson, Håkan and Sjögren, Hans-Olof}}, issn = {{1651-226X}}, language = {{eng}}, number = {{3}}, pages = {{309--312}}, publisher = {{Taylor & Francis}}, series = {{Acta Oncologica}}, title = {{Extracorporeal immunoadsorption from whole blood based on the avidin-biotin concept. Evaluation of a new method}}, url = {{http://dx.doi.org/10.3109/02841869609101646}}, doi = {{10.3109/02841869609101646}}, volume = {{35}}, year = {{1996}}, }