Very-low-density lipoprotein of uremic patients is a poor substrate for bovine lipoprotein lipase in vitro
(1996) In Metabolism, Clinical and Experimental 45(6). p.686-690- Abstract
- Very-low-density lipoprotein (VLDL) from 10 hemodialysis patients and 10 healthy controls was studied with respect to the substrate characteristics for bovine milk lipoprotein lipase (LPL). Compared with the control subjects, the hemodialysis patients had significantly higher serum triglyceride and apolipoprotein B-associated apolipoprotein CIII concentrations (1.03 +/- 0.31 v 1.98 +/- 0.86 mmol/L and 0.004 +/- 0.002 v 0.011 +/- 0.005 g/L, respectively), lower serum high-density lipoprotein (HDL) cholesterol and apolipoprotein AI concentrations (1.33 +/- 0.37 v 0.95 +/- 0.31 mmol/L and 1.29 +/- 0.25 v 1.09 +/- 0.23 g/L, respectively), and lower postheparin plasma LPL activity (82 +/- 24 v 35 +/- 14 milliU/milliL). There were also... (More)
- Very-low-density lipoprotein (VLDL) from 10 hemodialysis patients and 10 healthy controls was studied with respect to the substrate characteristics for bovine milk lipoprotein lipase (LPL). Compared with the control subjects, the hemodialysis patients had significantly higher serum triglyceride and apolipoprotein B-associated apolipoprotein CIII concentrations (1.03 +/- 0.31 v 1.98 +/- 0.86 mmol/L and 0.004 +/- 0.002 v 0.011 +/- 0.005 g/L, respectively), lower serum high-density lipoprotein (HDL) cholesterol and apolipoprotein AI concentrations (1.33 +/- 0.37 v 0.95 +/- 0.31 mmol/L and 1.29 +/- 0.25 v 1.09 +/- 0.23 g/L, respectively), and lower postheparin plasma LPL activity (82 +/- 24 v 35 +/- 14 milliU/milliL). There were also significant increases in the relative fat content and diameter of VLDL particles from patients versus controls. VLDL was labeled with a fluorescent phospholipid analog, DHPE, and the rate of the lipolytic reaction with purified bovine milk LPL was estimated from the increase in fluorescence intensity at 490 nm. There was no significant difference between initial reaction velocities in the study groups, but VLDL particles from hemodialysis patients were lipolyzed to a significantly lesser extent than those from healthy controls (mean increase in fluorescence intensity after completion of the reaction, 95 +/- 36 v 140 +/- 43 arbitrary units). These results are in accordance with the accumulation of remnant particles reported to occur in uremia despite only a moderately increased serum triglyceride concentration. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1110930
- author
- Arnadottir, Margret ; Dallongeville, Jean ; Fruchart, Jean-Charles and Nilsson-Ehle, Peter LU
- organization
- publishing date
- 1996
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Metabolism, Clinical and Experimental
- volume
- 45
- issue
- 6
- pages
- 686 - 690
- publisher
- Elsevier
- external identifiers
-
- pmid:8637441
- scopus:0029991688
- ISSN
- 1532-8600
- DOI
- 10.1016/S0026-0495(96)90132-8
- language
- English
- LU publication?
- yes
- id
- 6d66c120-46bf-4fb0-8570-445ce7d38b40 (old id 1110930)
- date added to LUP
- 2016-04-01 15:27:59
- date last changed
- 2022-01-28 05:27:12
@article{6d66c120-46bf-4fb0-8570-445ce7d38b40,
abstract = {{Very-low-density lipoprotein (VLDL) from 10 hemodialysis patients and 10 healthy controls was studied with respect to the substrate characteristics for bovine milk lipoprotein lipase (LPL). Compared with the control subjects, the hemodialysis patients had significantly higher serum triglyceride and apolipoprotein B-associated apolipoprotein CIII concentrations (1.03 +/- 0.31 v 1.98 +/- 0.86 mmol/L and 0.004 +/- 0.002 v 0.011 +/- 0.005 g/L, respectively), lower serum high-density lipoprotein (HDL) cholesterol and apolipoprotein AI concentrations (1.33 +/- 0.37 v 0.95 +/- 0.31 mmol/L and 1.29 +/- 0.25 v 1.09 +/- 0.23 g/L, respectively), and lower postheparin plasma LPL activity (82 +/- 24 v 35 +/- 14 milliU/milliL). There were also significant increases in the relative fat content and diameter of VLDL particles from patients versus controls. VLDL was labeled with a fluorescent phospholipid analog, DHPE, and the rate of the lipolytic reaction with purified bovine milk LPL was estimated from the increase in fluorescence intensity at 490 nm. There was no significant difference between initial reaction velocities in the study groups, but VLDL particles from hemodialysis patients were lipolyzed to a significantly lesser extent than those from healthy controls (mean increase in fluorescence intensity after completion of the reaction, 95 +/- 36 v 140 +/- 43 arbitrary units). These results are in accordance with the accumulation of remnant particles reported to occur in uremia despite only a moderately increased serum triglyceride concentration.}},
author = {{Arnadottir, Margret and Dallongeville, Jean and Fruchart, Jean-Charles and Nilsson-Ehle, Peter}},
issn = {{1532-8600}},
language = {{eng}},
number = {{6}},
pages = {{686--690}},
publisher = {{Elsevier}},
series = {{Metabolism, Clinical and Experimental}},
title = {{Very-low-density lipoprotein of uremic patients is a poor substrate for bovine lipoprotein lipase in vitro}},
url = {{http://dx.doi.org/10.1016/S0026-0495(96)90132-8}},
doi = {{10.1016/S0026-0495(96)90132-8}},
volume = {{45}},
year = {{1996}},
}