Release of endothelin-1 into rat airways following Sephadex-induced inflammation; modulation by enzyme inhibitors and budesonide
(1996) In Respiration 63(2). p.111-116- Abstract
- The intratracheal (i.t.) instillation of Sephadex beads into rat induced inflammation and a 30-fold increase in the endothelin-1-like immunoreactivity (ET-1-LI) of broncho-alveolar lavage fluid. The levels were highest 24 h after the instillation and had declined significantly after 48 h. At a dose of 1 mg kg-1 i.t., the glucocorticosteroid budesonide almost abolished this response. Phosphoramidon, which inhibits neutral endopeptidase, an enzyme reported to degrade ET-1 and also to inhibit the endothelin-converting enzyme, potentiated the Sephadex-induced rise in ET-1-LI. Chymostatin and heparin, which are reported to reduce the formation of ET-1, did not affect the increase in ET-1-LI. The present model represents a very reactive system... (More)
- The intratracheal (i.t.) instillation of Sephadex beads into rat induced inflammation and a 30-fold increase in the endothelin-1-like immunoreactivity (ET-1-LI) of broncho-alveolar lavage fluid. The levels were highest 24 h after the instillation and had declined significantly after 48 h. At a dose of 1 mg kg-1 i.t., the glucocorticosteroid budesonide almost abolished this response. Phosphoramidon, which inhibits neutral endopeptidase, an enzyme reported to degrade ET-1 and also to inhibit the endothelin-converting enzyme, potentiated the Sephadex-induced rise in ET-1-LI. Chymostatin and heparin, which are reported to reduce the formation of ET-1, did not affect the increase in ET-1-LI. The present model represents a very reactive system for analyzing the changes in ET-1 levels during inflammation. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1111108
- author
- Andersson, Sven LU ; Hemsen, A ; Zackrisson, C and Lundberg, J M
- organization
- publishing date
- 1996
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Respiration
- volume
- 63
- issue
- 2
- pages
- 111 - 116
- publisher
- Karger
- external identifiers
-
- pmid:8966364
- scopus:0029931775
- ISSN
- 1423-0356
- language
- English
- LU publication?
- yes
- id
- 2b2e5f37-0f36-4ef3-b1d6-30c57eafb13c (old id 1111108)
- date added to LUP
- 2016-04-01 16:41:32
- date last changed
- 2024-01-11 12:52:48
@article{2b2e5f37-0f36-4ef3-b1d6-30c57eafb13c, abstract = {{The intratracheal (i.t.) instillation of Sephadex beads into rat induced inflammation and a 30-fold increase in the endothelin-1-like immunoreactivity (ET-1-LI) of broncho-alveolar lavage fluid. The levels were highest 24 h after the instillation and had declined significantly after 48 h. At a dose of 1 mg kg-1 i.t., the glucocorticosteroid budesonide almost abolished this response. Phosphoramidon, which inhibits neutral endopeptidase, an enzyme reported to degrade ET-1 and also to inhibit the endothelin-converting enzyme, potentiated the Sephadex-induced rise in ET-1-LI. Chymostatin and heparin, which are reported to reduce the formation of ET-1, did not affect the increase in ET-1-LI. The present model represents a very reactive system for analyzing the changes in ET-1 levels during inflammation.}}, author = {{Andersson, Sven and Hemsen, A and Zackrisson, C and Lundberg, J M}}, issn = {{1423-0356}}, language = {{eng}}, number = {{2}}, pages = {{111--116}}, publisher = {{Karger}}, series = {{Respiration}}, title = {{Release of endothelin-1 into rat airways following Sephadex-induced inflammation; modulation by enzyme inhibitors and budesonide}}, volume = {{63}}, year = {{1996}}, }