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Adrenaline stimulates glucagon secretion in pancreatic A-cells by increasing the Ca2+ current and the number of granules close to the L-type Ca2+ channels

Gromada, J ; Bokvist, K ; Ding, W G ; Barg, Sebastian LU ; Buschard, K ; Renström, Erik LU and Rorsman, Patrik LU (1997) In Journal of General Physiology 110(3). p.217-228
Abstract
We have monitored electrical activity, voltage-gated Ca2+ currents, and exocytosis in single rat glucagon-secreting pancreatic A-cells. The A-cells were electrically excitable and generated spontaneous Na+- and Ca2+-dependent action potentials. Under basal conditions, exocytosis was tightly linked to Ca2+ influx through omega-conotoxin-GVIA-sensitive (N-type) Ca2+ channels. Stimulation of the A-cells with adrenaline (via beta-adrenergic receptors) or forskolin produced a greater than fourfold PKA-dependent potentiation of depolarization-evoked exocytosis. This enhancement of exocytosis was due to a 50% enhancement of Ca2+ influx through L-type Ca2+ channels, an effect that accounted for <30% of the total stimulatory action. The... (More)
We have monitored electrical activity, voltage-gated Ca2+ currents, and exocytosis in single rat glucagon-secreting pancreatic A-cells. The A-cells were electrically excitable and generated spontaneous Na+- and Ca2+-dependent action potentials. Under basal conditions, exocytosis was tightly linked to Ca2+ influx through omega-conotoxin-GVIA-sensitive (N-type) Ca2+ channels. Stimulation of the A-cells with adrenaline (via beta-adrenergic receptors) or forskolin produced a greater than fourfold PKA-dependent potentiation of depolarization-evoked exocytosis. This enhancement of exocytosis was due to a 50% enhancement of Ca2+ influx through L-type Ca2+ channels, an effect that accounted for <30% of the total stimulatory action. The remaining 70% of the stimulation was attributable to an acceleration of granule mobilization resulting in a fivefold increase in the number of readily releasable granules near the L-type Ca2+ channels. (Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
subject
keywords
glucagon, Ca2+, secretion
in
Journal of General Physiology
volume
110
issue
3
pages
217 - 228
publisher
Rockefeller University Press
external identifiers
  • pmid:9276750
  • scopus:0030967933
ISSN
0022-1295
language
English
LU publication?
no
id
ad7f60ac-295c-4a20-9607-523430576791 (old id 1111594)
alternative location
http://www.jgp.org/cgi/content/full/110/3/217
date added to LUP
2016-04-01 15:48:33
date last changed
2025-10-14 09:42:58
@article{ad7f60ac-295c-4a20-9607-523430576791,
  abstract     = {{We have monitored electrical activity, voltage-gated Ca2+ currents, and exocytosis in single rat glucagon-secreting pancreatic A-cells. The A-cells were electrically excitable and generated spontaneous Na+- and Ca2+-dependent action potentials. Under basal conditions, exocytosis was tightly linked to Ca2+ influx through omega-conotoxin-GVIA-sensitive (N-type) Ca2+ channels. Stimulation of the A-cells with adrenaline (via beta-adrenergic receptors) or forskolin produced a greater than fourfold PKA-dependent potentiation of depolarization-evoked exocytosis. This enhancement of exocytosis was due to a 50% enhancement of Ca2+ influx through L-type Ca2+ channels, an effect that accounted for &lt;30% of the total stimulatory action. The remaining 70% of the stimulation was attributable to an acceleration of granule mobilization resulting in a fivefold increase in the number of readily releasable granules near the L-type Ca2+ channels.}},
  author       = {{Gromada, J and Bokvist, K and Ding, W G and Barg, Sebastian and Buschard, K and Renström, Erik and Rorsman, Patrik}},
  issn         = {{0022-1295}},
  keywords     = {{glucagon; Ca2+; secretion}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{217--228}},
  publisher    = {{Rockefeller University Press}},
  series       = {{Journal of General Physiology}},
  title        = {{Adrenaline stimulates glucagon secretion in pancreatic A-cells by increasing the Ca2+ current and the number of granules close to the L-type Ca2+ channels}},
  url          = {{http://www.jgp.org/cgi/content/full/110/3/217}},
  volume       = {{110}},
  year         = {{1997}},
}