Expression profiling in cancer using cDNA microarrays
Khan, J ; Saal, Lao LU
; Bittner, M L
; Chen, Y
; Trent, J M
and Meltzer, P S
(1999)
In Electrophoresis
20(2).
p.223-229
- Abstract
- Currently there are over 1,000,000 human expressed sequence tag (EST) sequences available on the public database, representing perhaps 50-90% of all human genes. The cDNA microarray technique is a recently developed tool that exploits this wealth of information for the analysis of gene expression. In this method, DNA probes representing cDNA clones are arrayed onto a glass slide and interrogated with fluorescently labeled cDNA targets. The power of the technology is the ability to perform a genome-wide expression profile of thousands of genes in one experiment. In our review we describe the principles of the microarray technology as applied to cancer research, summarize the literature on its use so far, and speculate on the future... (More)
- Currently there are over 1,000,000 human expressed sequence tag (EST) sequences available on the public database, representing perhaps 50-90% of all human genes. The cDNA microarray technique is a recently developed tool that exploits this wealth of information for the analysis of gene expression. In this method, DNA probes representing cDNA clones are arrayed onto a glass slide and interrogated with fluorescently labeled cDNA targets. The power of the technology is the ability to perform a genome-wide expression profile of thousands of genes in one experiment. In our review we describe the principles of the microarray technology as applied to cancer research, summarize the literature on its use so far, and speculate on the future application of this powerful technique. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1114488
- author
- Khan, J
; Saal, Lao
LU
; Bittner, M L
; Chen, Y
; Trent, J M
and Meltzer, P S
- organization
- publishing date
- 1999
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Electrophoresis
- volume
- 20
- issue
- 2
- pages
- 223 - 229
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:10197427
- scopus:0033012697
- ISSN
- 0173-0835
- DOI
- 10.1002/(SICI)1522-2683(19990201)20:2<223::AID-ELPS223>3.0.CO;2-A
- language
- English
- LU publication?
- yes
- id
- ce3f6ed8-03f9-4d1a-a46a-359201bd2022 (old id 1114488)
- date added to LUP
- 2016-04-01 11:42:19
- date last changed
- 2022-01-26 17:00:42
@article{ce3f6ed8-03f9-4d1a-a46a-359201bd2022,
abstract = {{Currently there are over 1,000,000 human expressed sequence tag (EST) sequences available on the public database, representing perhaps 50-90% of all human genes. The cDNA microarray technique is a recently developed tool that exploits this wealth of information for the analysis of gene expression. In this method, DNA probes representing cDNA clones are arrayed onto a glass slide and interrogated with fluorescently labeled cDNA targets. The power of the technology is the ability to perform a genome-wide expression profile of thousands of genes in one experiment. In our review we describe the principles of the microarray technology as applied to cancer research, summarize the literature on its use so far, and speculate on the future application of this powerful technique.}},
author = {{Khan, J and Saal, Lao and Bittner, M L and Chen, Y and Trent, J M and Meltzer, P S}},
issn = {{0173-0835}},
language = {{eng}},
number = {{2}},
pages = {{223--229}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Electrophoresis}},
title = {{Expression profiling in cancer using cDNA microarrays}},
url = {{http://dx.doi.org/10.1002/(SICI)1522-2683(19990201)20:2<223::AID-ELPS223>3.0.CO;2-A}},
doi = {{10.1002/(SICI)1522-2683(19990201)20:2<223::AID-ELPS223>3.0.CO;2-A}},
volume = {{20}},
year = {{1999}},
}