IS1294, a DNA element that transposes by RC transposition
(2000) In Plasmid 44(1). p.66-84- Abstract
- IS1294, found on the ColD-like resistance plasmid pUB2380, is IS91-like. It is an active 1.7-kb insertion sequence that lacks terminal inverted repeats, displays insertion-site specificity, and does not generate direct repeats of the target site. The element has one large open reading frame, tnp(1294), encoding a transposase of 351 amino acids, related to members of the REP family of replication proteins used by RC-plasmids of gram-positive bacteria. IS1294 transposes using rolling-circle replication, initiated at one end of the element, oriIS, and terminated at the other, terIS. oriIS and terIS are highly conserved among like IS elements. oriIS resembles the leading strand replication origins of RC-plasmids; terIS resembles a... (More)
- IS1294, found on the ColD-like resistance plasmid pUB2380, is IS91-like. It is an active 1.7-kb insertion sequence that lacks terminal inverted repeats, displays insertion-site specificity, and does not generate direct repeats of the target site. The element has one large open reading frame, tnp(1294), encoding a transposase of 351 amino acids, related to members of the REP family of replication proteins used by RC-plasmids of gram-positive bacteria. IS1294 transposes using rolling-circle replication, initiated at one end of the element, oriIS, and terminated at the other, terIS. oriIS and terIS are highly conserved among like IS elements. oriIS resembles the leading strand replication origins of RC-plasmids; terIS resembles a rho-independent transcription terminator. IS1294 mediates not only its own transposition, but also sequences adjacent to terIS. A transposition model for IS1294 and related elements, involving rolling-circle replication and single-strand DNA intermediates, is presented. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1116300
- author
- Tavakoli, N ; Comanducci, A ; Dodd, H M ; Lett, M C ; Albiger, Barbara LU and Bennett, P
- publishing date
- 2000
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Plasmid
- volume
- 44
- issue
- 1
- pages
- 66 - 84
- publisher
- Academic Press
- external identifiers
-
- pmid:10873528
- scopus:0033912362
- ISSN
- 0147-619X
- DOI
- 10.1006/plas.1999.1460
- language
- English
- LU publication?
- no
- id
- 013bd037-2823-456f-8521-3564ec23570d (old id 1116300)
- date added to LUP
- 2016-04-01 12:32:17
- date last changed
- 2022-01-27 06:27:30
@article{013bd037-2823-456f-8521-3564ec23570d,
abstract = {{IS1294, found on the ColD-like resistance plasmid pUB2380, is IS91-like. It is an active 1.7-kb insertion sequence that lacks terminal inverted repeats, displays insertion-site specificity, and does not generate direct repeats of the target site. The element has one large open reading frame, tnp(1294), encoding a transposase of 351 amino acids, related to members of the REP family of replication proteins used by RC-plasmids of gram-positive bacteria. IS1294 transposes using rolling-circle replication, initiated at one end of the element, oriIS, and terminated at the other, terIS. oriIS and terIS are highly conserved among like IS elements. oriIS resembles the leading strand replication origins of RC-plasmids; terIS resembles a rho-independent transcription terminator. IS1294 mediates not only its own transposition, but also sequences adjacent to terIS. A transposition model for IS1294 and related elements, involving rolling-circle replication and single-strand DNA intermediates, is presented.}},
author = {{Tavakoli, N and Comanducci, A and Dodd, H M and Lett, M C and Albiger, Barbara and Bennett, P}},
issn = {{0147-619X}},
language = {{eng}},
number = {{1}},
pages = {{66--84}},
publisher = {{Academic Press}},
series = {{Plasmid}},
title = {{IS1294, a DNA element that transposes by RC transposition}},
url = {{http://dx.doi.org/10.1006/plas.1999.1460}},
doi = {{10.1006/plas.1999.1460}},
volume = {{44}},
year = {{2000}},
}