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First-generation monoclonal antibodies identifying the human leukotriene B(4) receptor-1

Pettersson, Annika LU ; Boketoft, Åke LU ; Sabirsh, Alan LU ; Nilsson, Niclas LU ; Kotarsky, Knut LU ; Olde, Björn LU and Owman, Christer LU (2000) In Biochemical and Biophysical Research Communications 279(2). p.520-525
Abstract
The leukotriene B(4) receptor (BLTR) is a seven-transmembrane chemoattractant receptor that is important in pro-inflammatory responses. We have produced the first widely applicable monoclonal antibodies against the human BLTR and confirmed the antibody specificity using flow cytometric analysis of three different cell lines stably expressing the recombinant receptor. The antibodies did not cross-react with the recently cloned second LTB(4) receptor, BLTR2, or the Cys LT1 and Cys LT2 receptors. Functional analysis in combination with two-color flow cytometry showed that the BLTR antibodies bind to cells that are activated by LTB(4). The antibodies were shown to recognize BLTR in cell ELISA and immunocytochemistry. Endogenous expression of... (More)
The leukotriene B(4) receptor (BLTR) is a seven-transmembrane chemoattractant receptor that is important in pro-inflammatory responses. We have produced the first widely applicable monoclonal antibodies against the human BLTR and confirmed the antibody specificity using flow cytometric analysis of three different cell lines stably expressing the recombinant receptor. The antibodies did not cross-react with the recently cloned second LTB(4) receptor, BLTR2, or the Cys LT1 and Cys LT2 receptors. Functional analysis in combination with two-color flow cytometry showed that the BLTR antibodies bind to cells that are activated by LTB(4). The antibodies were shown to recognize BLTR in cell ELISA and immunocytochemistry. Endogenous expression of BLTR in CD15-positive blood leukocytes and in differentiated HL-60 cells was also demonstrated with the antibodies. (Less)
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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
leukotriene B4 receptor, BLTR, recombinant receptor, endogenous receptor, leukocytes, HL-60 cells, monoclonal antibodies
in
Biochemical and Biophysical Research Communications
volume
279
issue
2
pages
520 - 525
publisher
Elsevier
external identifiers
  • pmid:11118319
  • scopus:0034694826
ISSN
1090-2104
DOI
10.1006/bbrc.2000.3989
language
English
LU publication?
yes
id
2a804c41-70a2-4a73-a38d-1c6c59465997 (old id 1116314)
date added to LUP
2016-04-01 15:33:54
date last changed
2022-01-28 05:59:58
@article{2a804c41-70a2-4a73-a38d-1c6c59465997,
  abstract     = {{The leukotriene B(4) receptor (BLTR) is a seven-transmembrane chemoattractant receptor that is important in pro-inflammatory responses. We have produced the first widely applicable monoclonal antibodies against the human BLTR and confirmed the antibody specificity using flow cytometric analysis of three different cell lines stably expressing the recombinant receptor. The antibodies did not cross-react with the recently cloned second LTB(4) receptor, BLTR2, or the Cys LT1 and Cys LT2 receptors. Functional analysis in combination with two-color flow cytometry showed that the BLTR antibodies bind to cells that are activated by LTB(4). The antibodies were shown to recognize BLTR in cell ELISA and immunocytochemistry. Endogenous expression of BLTR in CD15-positive blood leukocytes and in differentiated HL-60 cells was also demonstrated with the antibodies.}},
  author       = {{Pettersson, Annika and Boketoft, Åke and Sabirsh, Alan and Nilsson, Niclas and Kotarsky, Knut and Olde, Björn and Owman, Christer}},
  issn         = {{1090-2104}},
  keywords     = {{leukotriene B4 receptor; BLTR; recombinant receptor; endogenous receptor; leukocytes; HL-60 cells; monoclonal antibodies}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{520--525}},
  publisher    = {{Elsevier}},
  series       = {{Biochemical and Biophysical Research Communications}},
  title        = {{First-generation monoclonal antibodies identifying the human leukotriene B(4) receptor-1}},
  url          = {{http://dx.doi.org/10.1006/bbrc.2000.3989}},
  doi          = {{10.1006/bbrc.2000.3989}},
  volume       = {{279}},
  year         = {{2000}},
}