Cloning and characterization of cDNA encoding a novel human leukotriene B(4) receptor
(2000) In Biochemical and Biophysical Research Communications 274(2). p.377-382- Abstract
- By homology screening using BLAST searches of expressed sequence tags (ESTs), we have found a previously unidentified cDNA encoding a putative seven-transmembrane receptor with highest similarity to the leukotriene B(4) receptor, BLTR. Analysis of calcium flow in transfected cells, along with sequence analysis, revealed that the EST encoded a functionally inactive protein, lacking the segment corresponding to the C-terminal part of the putative receptor protein. The missing segment was obtained by PCR amplification of a human leukocyte cDNA library and ligated to the truncated EST cDNA. The novel cDNA encodes a full-length receptor with 39% identity to the previously cloned BLTR. Studies of intracellular calcium flow of transfected HeLa... (More)
- By homology screening using BLAST searches of expressed sequence tags (ESTs), we have found a previously unidentified cDNA encoding a putative seven-transmembrane receptor with highest similarity to the leukotriene B(4) receptor, BLTR. Analysis of calcium flow in transfected cells, along with sequence analysis, revealed that the EST encoded a functionally inactive protein, lacking the segment corresponding to the C-terminal part of the putative receptor protein. The missing segment was obtained by PCR amplification of a human leukocyte cDNA library and ligated to the truncated EST cDNA. The novel cDNA encodes a full-length receptor with 39% identity to the previously cloned BLTR. Studies of intracellular calcium flow of transfected HeLa cells exposed to various leukotrienes showed that also the novel BLTR-like receptor can be activated by leukotriene B(4), and it is therefore tentatively named BLTR2. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1116321
- author
- Tryselius, Ylva LU ; Nilsson, Niclas LU ; Kotarsky, Knut LU ; Olde, Björn LU and Owman, Christer LU
- organization
- publishing date
- 2000
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- leukotriene B4, BLTR2, BLTR, calcium assay, 7TM receptor
- in
- Biochemical and Biophysical Research Communications
- volume
- 274
- issue
- 2
- pages
- 377 - 382
- publisher
- Elsevier
- external identifiers
-
- pmid:10913346
- scopus:0034596213
- ISSN
- 1090-2104
- DOI
- 10.1006/bbrc.2000.3152
- language
- English
- LU publication?
- yes
- id
- 91df6b61-d735-453a-9c3b-e35c088ebe9f (old id 1116321)
- date added to LUP
- 2016-04-01 15:18:32
- date last changed
- 2022-03-22 03:47:31
@article{91df6b61-d735-453a-9c3b-e35c088ebe9f, abstract = {{By homology screening using BLAST searches of expressed sequence tags (ESTs), we have found a previously unidentified cDNA encoding a putative seven-transmembrane receptor with highest similarity to the leukotriene B(4) receptor, BLTR. Analysis of calcium flow in transfected cells, along with sequence analysis, revealed that the EST encoded a functionally inactive protein, lacking the segment corresponding to the C-terminal part of the putative receptor protein. The missing segment was obtained by PCR amplification of a human leukocyte cDNA library and ligated to the truncated EST cDNA. The novel cDNA encodes a full-length receptor with 39% identity to the previously cloned BLTR. Studies of intracellular calcium flow of transfected HeLa cells exposed to various leukotrienes showed that also the novel BLTR-like receptor can be activated by leukotriene B(4), and it is therefore tentatively named BLTR2.}}, author = {{Tryselius, Ylva and Nilsson, Niclas and Kotarsky, Knut and Olde, Björn and Owman, Christer}}, issn = {{1090-2104}}, keywords = {{leukotriene B4; BLTR2; BLTR; calcium assay; 7TM receptor}}, language = {{eng}}, number = {{2}}, pages = {{377--382}}, publisher = {{Elsevier}}, series = {{Biochemical and Biophysical Research Communications}}, title = {{Cloning and characterization of cDNA encoding a novel human leukotriene B(4) receptor}}, url = {{http://dx.doi.org/10.1006/bbrc.2000.3152}}, doi = {{10.1006/bbrc.2000.3152}}, volume = {{274}}, year = {{2000}}, }