Airborne cell analysis
Santesson, Sabina LU ; Andersson, Martin ; Degerman, Eva LU
; Johansson, Thomas
LU
; Nilsson, Johan
LU
and Nilsson, Staffan
LU
(2000)
In Analytical Chemistry
72(15).
p.3412-3418
- Abstract
- A miniaturized analysis system for the study of living cells and biochemical reactions in microdroplets was developed. The technique utilizes an in-house-developed piezoelectric flow-through droplet dispenser for precise reagent supply and an ultrasonic levitator for contactless sample handling. A few-cell study was performed with living primary adipocytes. Droplets (500 nL) containing 3-15 individual cells were acoustically levitated. The addition of beta-adrenergic agonists into the levitated droplet using the droplet dispenser stimulated adipocyte lipolysis, leading to free fatty acid release and a consequent pH decrease of the surrounding buffer. The addition of insulin antagonized lipolysis and hence also the decrease in pH. The... (More)
- A miniaturized analysis system for the study of living cells and biochemical reactions in microdroplets was developed. The technique utilizes an in-house-developed piezoelectric flow-through droplet dispenser for precise reagent supply and an ultrasonic levitator for contactless sample handling. A few-cell study was performed with living primary adipocytes. Droplets (500 nL) containing 3-15 individual cells were acoustically levitated. The addition of beta-adrenergic agonists into the levitated droplet using the droplet dispenser stimulated adipocyte lipolysis, leading to free fatty acid release and a consequent pH decrease of the surrounding buffer. The addition of insulin antagonized lipolysis and hence also the decrease in pH. The changes in pH, i.e., the cell response in the droplet, were followed using a pH-dependent fluorophore continuously monitored by fluorescence imaging detection. An image analysis computer program was employed to calculate the droplet intensities. To counteract droplet evaporation, found to affect the fluorescence intensities, a separate dispenser was used to continually add water, thus keeping the droplet volume constant. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1117953
- author
- Santesson, Sabina
LU
; Andersson, Martin
; Degerman, Eva
LU
; Johansson, Thomas
LU
; Nilsson, Johan
LU
and Nilsson, Staffan
LU
- organization
- publishing date
- 2000
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analytical Chemistry
- volume
- 72
- issue
- 15
- pages
- 3412 - 3418
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- pmid:10952520
- scopus:0034254832
- ISSN
- 1520-6882
- DOI
- 10.1021/ac000140r
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Insulin Signal Transduction (013212014), Analytical Chemistry (S/LTH) (011001004), Biomedical Engineering (011200011), Atomic physics (011013005)
- id
- b0fd5003-5ff2-4d09-a263-fb2b310b4cf7 (old id 1117953)
- date added to LUP
- 2016-04-01 12:05:01
- date last changed
- 2022-04-21 02:14:43
@article{b0fd5003-5ff2-4d09-a263-fb2b310b4cf7,
abstract = {{A miniaturized analysis system for the study of living cells and biochemical reactions in microdroplets was developed. The technique utilizes an in-house-developed piezoelectric flow-through droplet dispenser for precise reagent supply and an ultrasonic levitator for contactless sample handling. A few-cell study was performed with living primary adipocytes. Droplets (500 nL) containing 3-15 individual cells were acoustically levitated. The addition of beta-adrenergic agonists into the levitated droplet using the droplet dispenser stimulated adipocyte lipolysis, leading to free fatty acid release and a consequent pH decrease of the surrounding buffer. The addition of insulin antagonized lipolysis and hence also the decrease in pH. The changes in pH, i.e., the cell response in the droplet, were followed using a pH-dependent fluorophore continuously monitored by fluorescence imaging detection. An image analysis computer program was employed to calculate the droplet intensities. To counteract droplet evaporation, found to affect the fluorescence intensities, a separate dispenser was used to continually add water, thus keeping the droplet volume constant.}},
author = {{Santesson, Sabina and Andersson, Martin and Degerman, Eva and Johansson, Thomas and Nilsson, Johan and Nilsson, Staffan}},
issn = {{1520-6882}},
language = {{eng}},
number = {{15}},
pages = {{3412--3418}},
publisher = {{The American Chemical Society (ACS)}},
series = {{Analytical Chemistry}},
title = {{Airborne cell analysis}},
url = {{https://lup.lub.lu.se/search/files/2773965/2371360.pdf}},
doi = {{10.1021/ac000140r}},
volume = {{72}},
year = {{2000}},
}