BRCA1-mediated repression of select X chromosome genes
(2004) In Journal of Translational Medicine 2(1).- Abstract
- Recently BRCA1 has been implicated in the regulation of gene expression from the X chromosome. In this study the influence of BRCA1 on expression of X chromosome genes was investigated. Complementary DNA microarrays were used to compare the expression levels of X chromosome genes in 18 BRCA1-associated ovarian cancers to those of the 13 "BRCA1-like" and 14 "BRCA2-like" sporadic tumors (as defined by previously reported expression profiling). Significance was determined using parametric statistics with P < 0.005 as a cutoff. Forty of 178 total X-chromosome transcripts were differentially expressed between the BRCA1-associated tumors and sporadic cancers with a BRCA2-like molecular profile. Thirty of these 40 genes showed higher mean... (More)
- Recently BRCA1 has been implicated in the regulation of gene expression from the X chromosome. In this study the influence of BRCA1 on expression of X chromosome genes was investigated. Complementary DNA microarrays were used to compare the expression levels of X chromosome genes in 18 BRCA1-associated ovarian cancers to those of the 13 "BRCA1-like" and 14 "BRCA2-like" sporadic tumors (as defined by previously reported expression profiling). Significance was determined using parametric statistics with P < 0.005 as a cutoff. Forty of 178 total X-chromosome transcripts were differentially expressed between the BRCA1-associated tumors and sporadic cancers with a BRCA2-like molecular profile. Thirty of these 40 genes showed higher mean expression in the BRCA1-associated samples including all 11 transcripts that mapped to Xp11. In contrast, four of 178 total X chromosome transcripts showed significant differential expression between BRCA1-associated and sporadic tumors with a BRCA1-like molecular profile. All four mapped to Xp11 and showed higher mean expression in BRCA1-associated tumors. Re-expression of BRCA1 in HCC1937 BRCA1-deficient breast cancer cell resulted in the repression of 21 transcripts. Eleven of the 21 (54.5%) transcripts mapped to Xp11. However, there was no significant overlap between these Xp11 genes and those found to be differentially expressed between BRCA1-associated and sporadic ovarian cancer samples. These results demonstrate that BRCA1 mediates the repression of several X chromosome genes, many of which map to the Xp11 locus. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1131012
- author
- Jazaeri, Amir A ; Chandramouli, Gadisetti VR ; Aprelikova, Olga ; Nuber, Ulrike LU ; Sotiriou, Christos ; Liu, Edison T ; Ropers, H Hilger ; Yee, Cindy J ; Boyd, Jeff and Barrett, J Carl
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Translational Medicine
- volume
- 2
- issue
- 1
- article number
- 32
- publisher
- BioMed Central (BMC)
- external identifiers
-
- pmid:15383145
- scopus:11044236365
- pmid:15383145
- ISSN
- 1479-5876
- DOI
- 10.1186/1479-5876-2-32
- language
- English
- LU publication?
- no
- id
- a42f31fc-50e9-494b-a1e8-5f6417ae4db9 (old id 1131012)
- date added to LUP
- 2016-04-04 09:40:56
- date last changed
- 2022-03-31 07:38:52
@article{a42f31fc-50e9-494b-a1e8-5f6417ae4db9, abstract = {{Recently BRCA1 has been implicated in the regulation of gene expression from the X chromosome. In this study the influence of BRCA1 on expression of X chromosome genes was investigated. Complementary DNA microarrays were used to compare the expression levels of X chromosome genes in 18 BRCA1-associated ovarian cancers to those of the 13 "BRCA1-like" and 14 "BRCA2-like" sporadic tumors (as defined by previously reported expression profiling). Significance was determined using parametric statistics with P < 0.005 as a cutoff. Forty of 178 total X-chromosome transcripts were differentially expressed between the BRCA1-associated tumors and sporadic cancers with a BRCA2-like molecular profile. Thirty of these 40 genes showed higher mean expression in the BRCA1-associated samples including all 11 transcripts that mapped to Xp11. In contrast, four of 178 total X chromosome transcripts showed significant differential expression between BRCA1-associated and sporadic tumors with a BRCA1-like molecular profile. All four mapped to Xp11 and showed higher mean expression in BRCA1-associated tumors. Re-expression of BRCA1 in HCC1937 BRCA1-deficient breast cancer cell resulted in the repression of 21 transcripts. Eleven of the 21 (54.5%) transcripts mapped to Xp11. However, there was no significant overlap between these Xp11 genes and those found to be differentially expressed between BRCA1-associated and sporadic ovarian cancer samples. These results demonstrate that BRCA1 mediates the repression of several X chromosome genes, many of which map to the Xp11 locus.}}, author = {{Jazaeri, Amir A and Chandramouli, Gadisetti VR and Aprelikova, Olga and Nuber, Ulrike and Sotiriou, Christos and Liu, Edison T and Ropers, H Hilger and Yee, Cindy J and Boyd, Jeff and Barrett, J Carl}}, issn = {{1479-5876}}, language = {{eng}}, number = {{1}}, publisher = {{BioMed Central (BMC)}}, series = {{Journal of Translational Medicine}}, title = {{BRCA1-mediated repression of select X chromosome genes}}, url = {{http://dx.doi.org/10.1186/1479-5876-2-32}}, doi = {{10.1186/1479-5876-2-32}}, volume = {{2}}, year = {{2004}}, }