3-Hydroxy fatty acids in faeces of patients with Crohn's disease or ulcerative colitis as a non-invasive diagnostic approach
(2007) In Microbial Ecology in Health and Disease 19. p.1-6- Abstract
- The microbiota is part of the gastrointestinal ecosystem. A more detailed understanding should provide insight into multiple human disease states. This study investigated inflammatory bowel disease (Crohn's disease and ulcerative colitis). Previous analyses have suggested a role of gram-negative bacteria including Escherichia coli. An integrated procedure is presented where gas chromatography-mass spectrometry is used to determine chemical markers of gram-negative bacterial lipopolysaccharide (3-hydroxy fatty acids with 10-18 carbon atoms) in faecal samples. Six patients with Crohn's disease (CD), five with ulcerative colitis (UC) and six healthy adults were chosen as groups of interest. Nine saturated straight-chain 3-OH fatty acids of... (More)
- The microbiota is part of the gastrointestinal ecosystem. A more detailed understanding should provide insight into multiple human disease states. This study investigated inflammatory bowel disease (Crohn's disease and ulcerative colitis). Previous analyses have suggested a role of gram-negative bacteria including Escherichia coli. An integrated procedure is presented where gas chromatography-mass spectrometry is used to determine chemical markers of gram-negative bacterial lipopolysaccharide (3-hydroxy fatty acids with 10-18 carbon atoms) in faecal samples. Six patients with Crohn's disease (CD), five with ulcerative colitis (UC) and six healthy adults were chosen as groups of interest. Nine saturated straight-chain 3-OH fatty acids of 10-18 carbon chain lengths and six iso- and anteiso-branched-chain 3-OH fatty acids of 15-18 carbon chain lengths were detected. Significant differences were found in the 3-OH n-C17:0, 3-OH i-C18:0 and 3-OH n-C18:0 composition of faeces. The present study therefore confirms that alteration of the composition of the endogenous gram-negative microbiota may be of importance in inflammatory bowel disease and those alterations could be detected with a non-invasive chemical-analytical approach. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1140301
- author
- de la Cochetiére, Marie France ; Rougé, Carol ; Szponar, Bogumila ; Larsson, Lennart LU and Potel, Gilles
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- faecal microbiota, gas chromatography-mass spectrometry, inflammatory bowel diseases
- in
- Microbial Ecology in Health and Disease
- volume
- 19
- pages
- 1 - 6
- publisher
- Taylor & Francis
- external identifiers
-
- scopus:34147102344
- ISSN
- 0891-060X
- DOI
- 10.1080/08910600601132615
- language
- English
- LU publication?
- yes
- id
- 9d774cb8-17ad-47b5-aac3-122c739f142e (old id 1140301)
- date added to LUP
- 2016-04-01 11:53:45
- date last changed
- 2022-01-26 19:48:23
@article{9d774cb8-17ad-47b5-aac3-122c739f142e,
abstract = {{The microbiota is part of the gastrointestinal ecosystem. A more detailed understanding should provide insight into multiple human disease states. This study investigated inflammatory bowel disease (Crohn's disease and ulcerative colitis). Previous analyses have suggested a role of gram-negative bacteria including Escherichia coli. An integrated procedure is presented where gas chromatography-mass spectrometry is used to determine chemical markers of gram-negative bacterial lipopolysaccharide (3-hydroxy fatty acids with 10-18 carbon atoms) in faecal samples. Six patients with Crohn's disease (CD), five with ulcerative colitis (UC) and six healthy adults were chosen as groups of interest. Nine saturated straight-chain 3-OH fatty acids of 10-18 carbon chain lengths and six iso- and anteiso-branched-chain 3-OH fatty acids of 15-18 carbon chain lengths were detected. Significant differences were found in the 3-OH n-C17:0, 3-OH i-C18:0 and 3-OH n-C18:0 composition of faeces. The present study therefore confirms that alteration of the composition of the endogenous gram-negative microbiota may be of importance in inflammatory bowel disease and those alterations could be detected with a non-invasive chemical-analytical approach.}},
author = {{de la Cochetiére, Marie France and Rougé, Carol and Szponar, Bogumila and Larsson, Lennart and Potel, Gilles}},
issn = {{0891-060X}},
keywords = {{faecal microbiota; gas chromatography-mass spectrometry; inflammatory bowel diseases}},
language = {{eng}},
pages = {{1--6}},
publisher = {{Taylor & Francis}},
series = {{Microbial Ecology in Health and Disease}},
title = {{3-Hydroxy fatty acids in faeces of patients with Crohn's disease or ulcerative colitis as a non-invasive diagnostic approach}},
url = {{http://dx.doi.org/10.1080/08910600601132615}},
doi = {{10.1080/08910600601132615}},
volume = {{19}},
year = {{2007}},
}