Slalom encodes an adenosine 3'-phosphate 5'-phosphosulfate transporter essential for development in Drosophila.
(2003) In EMBO Journal 22(14). p.3635-3644- Abstract
- Sulfation of all macromolecules entering the secretory pathway in higher organisms occurs in the Golgi and requires the high-energy sulfate donor adenosine 3'-phosphate 5'-phosphosulfate. Here we report the first molecular identification of a gene that encodes a transmembrane protein required to transport adenosine 3'-phosphate 5'-phosphosulfate from the cytosol into the Golgi lumen. Mutations in this gene, which we call slalom, display defects in Wg and Hh signaling, which are likely due to the lack of sulfation of glycos aminoglycans by the sulfotransferase sulfateless. Analysis of mosaic mutant ovaries shows that sll function is also essential for dorsal–ventral axis determination, suggesting that sll transports the sulfate donor... (More)
- Sulfation of all macromolecules entering the secretory pathway in higher organisms occurs in the Golgi and requires the high-energy sulfate donor adenosine 3'-phosphate 5'-phosphosulfate. Here we report the first molecular identification of a gene that encodes a transmembrane protein required to transport adenosine 3'-phosphate 5'-phosphosulfate from the cytosol into the Golgi lumen. Mutations in this gene, which we call slalom, display defects in Wg and Hh signaling, which are likely due to the lack of sulfation of glycos aminoglycans by the sulfotransferase sulfateless. Analysis of mosaic mutant ovaries shows that sll function is also essential for dorsal–ventral axis determination, suggesting that sll transports the sulfate donor required for sulfotransferase activity of the dorsal–ventral determinant pipe. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/116467
- author
- Lueders, Florian LU ; Segawa, Hiroaki ; Stein, David ; Selva, Erica M. ; Perrimon, Norbert ; Turco, Salvatore J. and Häcker, Udo LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- sulfation, Pipe, glycosaminoglycan, Hedgehog, Wingless
- in
- EMBO Journal
- volume
- 22
- issue
- 14
- pages
- 3635 - 3644
- publisher
- Oxford University Press
- external identifiers
-
- wos:000184385700017
- pmid:12853478
- scopus:0041813261
- pmid:12853478
- ISSN
- 1460-2075
- DOI
- 10.1093/emboj/cdg345
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Developmental biology (LUR000007), Invertebral Developmental Biology, Udo Haecker's group (013212048)
- id
- 4d86688c-9fe9-4edb-a133-c3abc50ac61f (old id 116467)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12853478&dopt=Abstract
- date added to LUP
- 2016-04-01 15:57:33
- date last changed
- 2022-02-20 02:18:37
@article{4d86688c-9fe9-4edb-a133-c3abc50ac61f, abstract = {{Sulfation of all macromolecules entering the secretory pathway in higher organisms occurs in the Golgi and requires the high-energy sulfate donor adenosine 3'-phosphate 5'-phosphosulfate. Here we report the first molecular identification of a gene that encodes a transmembrane protein required to transport adenosine 3'-phosphate 5'-phosphosulfate from the cytosol into the Golgi lumen. Mutations in this gene, which we call slalom, display defects in Wg and Hh signaling, which are likely due to the lack of sulfation of glycos aminoglycans by the sulfotransferase sulfateless. Analysis of mosaic mutant ovaries shows that sll function is also essential for dorsal–ventral axis determination, suggesting that sll transports the sulfate donor required for sulfotransferase activity of the dorsal–ventral determinant pipe.}}, author = {{Lueders, Florian and Segawa, Hiroaki and Stein, David and Selva, Erica M. and Perrimon, Norbert and Turco, Salvatore J. and Häcker, Udo}}, issn = {{1460-2075}}, keywords = {{sulfation; Pipe; glycosaminoglycan; Hedgehog; Wingless}}, language = {{eng}}, number = {{14}}, pages = {{3635--3644}}, publisher = {{Oxford University Press}}, series = {{EMBO Journal}}, title = {{Slalom encodes an adenosine 3'-phosphate 5'-phosphosulfate transporter essential for development in Drosophila.}}, url = {{http://dx.doi.org/10.1093/emboj/cdg345}}, doi = {{10.1093/emboj/cdg345}}, volume = {{22}}, year = {{2003}}, }