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Application of supercritical carbon dioxide(scCO(2))for the extraction of lipopolysaccharides (LPS) from Salmonella enterica subsp enterica PCM 2266

Rybka, Jacek ; Grycko, Pawel ; da Cruz Francisco, José LU ; Gamian, Andrzej and Dey, Estera LU (2008) In Journal of Supercritical Fluids 45(1). p.51-56
Abstract
The paper describes a novel extraction procedure for lipopolysaccharides (LPS) from Salmonella enterica subsp. enterica (PCM 2266). Process parameters for the extraction of LPS from bacterial mass were optimized by carrying out a two-level fractional design experiment. Four parameters, namely temperature, CO2 flow rate, pressure and co-solvent composition were analyzed. The best crude extract yields were achieved when the CO2 flow rate and temperature were kept high (10 g/min, 90 degrees C) and pure water was used as a co-solvent. Pressure had no statistically significant effect within the range of the study performed, whereas the other factors were relevant. The recovery of the extracted LPS by scCO(2) was about 3.3% of the biomass used,... (More)
The paper describes a novel extraction procedure for lipopolysaccharides (LPS) from Salmonella enterica subsp. enterica (PCM 2266). Process parameters for the extraction of LPS from bacterial mass were optimized by carrying out a two-level fractional design experiment. Four parameters, namely temperature, CO2 flow rate, pressure and co-solvent composition were analyzed. The best crude extract yields were achieved when the CO2 flow rate and temperature were kept high (10 g/min, 90 degrees C) and pure water was used as a co-solvent. Pressure had no statistically significant effect within the range of the study performed, whereas the other factors were relevant. The recovery of the extracted LPS by scCO(2) was about 3.3% of the biomass used, while in the classical extraction method yield was less than 2%. All isolates were characterized by SDS-PAGE, by the spectra of the thiobarbituric acid reaction products and GLC-MS analysis. (Less)
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
vaccine, Salmonella, lipopolysaccharides (LPS), supercritical carbon dioxide (scCO(2))
in
Journal of Supercritical Fluids
volume
45
issue
1
pages
51 - 56
publisher
Elsevier
external identifiers
  • wos:000255297400006
  • scopus:40949108080
ISSN
0896-8446
DOI
10.1016/j.supflu.2007.11.011
language
English
LU publication?
yes
id
757e3560-2db8-4142-96b9-c08f8d4a15dd (old id 1205253)
date added to LUP
2016-04-01 12:16:54
date last changed
2025-10-14 10:48:20
@article{757e3560-2db8-4142-96b9-c08f8d4a15dd,
  abstract     = {{The paper describes a novel extraction procedure for lipopolysaccharides (LPS) from Salmonella enterica subsp. enterica (PCM 2266). Process parameters for the extraction of LPS from bacterial mass were optimized by carrying out a two-level fractional design experiment. Four parameters, namely temperature, CO2 flow rate, pressure and co-solvent composition were analyzed. The best crude extract yields were achieved when the CO2 flow rate and temperature were kept high (10 g/min, 90 degrees C) and pure water was used as a co-solvent. Pressure had no statistically significant effect within the range of the study performed, whereas the other factors were relevant. The recovery of the extracted LPS by scCO(2) was about 3.3% of the biomass used, while in the classical extraction method yield was less than 2%. All isolates were characterized by SDS-PAGE, by the spectra of the thiobarbituric acid reaction products and GLC-MS analysis.}},
  author       = {{Rybka, Jacek and Grycko, Pawel and da Cruz Francisco, José and Gamian, Andrzej and Dey, Estera}},
  issn         = {{0896-8446}},
  keywords     = {{vaccine; Salmonella; lipopolysaccharides (LPS); supercritical carbon dioxide (scCO(2))}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{51--56}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Supercritical Fluids}},
  title        = {{Application of supercritical carbon dioxide(scCO(2))for the extraction of lipopolysaccharides (LPS) from Salmonella enterica subsp enterica PCM 2266}},
  url          = {{http://dx.doi.org/10.1016/j.supflu.2007.11.011}},
  doi          = {{10.1016/j.supflu.2007.11.011}},
  volume       = {{45}},
  year         = {{2008}},
}