Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

The C-terminus of dUTPase: observation on flexibility using NMR

Nord, Johan ; Nyman, Per-Olof LU ; Larsson, Gunilla and Drakenberg, Torbjörn LU (2001) In FEBS Letters 492(3). p.228-232
Abstract
The dynamics of the C-terminus of the dUTPases from Escherichia coli and equine infectious anaemia virus (EIAV) were studied by 1H-15N nuclear magnetic resonance spectroscopy. The two enzymes differ with regard to flexibility in the backbone of the 15 most C-terminal amino acid residues, some of which are conserved and essential for enzymic activity. In the bacterial enzyme, the residues closest to the C-terminus are highly flexible and display a correlation time in the nanosecond time range. No similar high flexibility could be detected for the C-terminal part of EIAV dUTPase, indicating a different time range of flexibility.
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
dUTPase, Equine infectious anemia virus, Flexible C-terminus, Nuclear magnetic resonance, Nucleotide binding, Escherichia coli
in
FEBS Letters
volume
492
issue
3
pages
228 - 232
publisher
Wiley-Blackwell
external identifiers
  • scopus:0035896405
ISSN
1873-3468
DOI
10.1016/S0014-5793(01)02257-8
language
English
LU publication?
yes
id
9e09bd37-b249-46bc-ba6f-92ef2d4e36b9 (old id 125038)
date added to LUP
2016-04-01 16:16:15
date last changed
2022-01-28 18:28:39
@article{9e09bd37-b249-46bc-ba6f-92ef2d4e36b9,
  abstract     = {{The dynamics of the C-terminus of the dUTPases from Escherichia coli and equine infectious anaemia virus (EIAV) were studied by 1H-15N nuclear magnetic resonance spectroscopy. The two enzymes differ with regard to flexibility in the backbone of the 15 most C-terminal amino acid residues, some of which are conserved and essential for enzymic activity. In the bacterial enzyme, the residues closest to the C-terminus are highly flexible and display a correlation time in the nanosecond time range. No similar high flexibility could be detected for the C-terminal part of EIAV dUTPase, indicating a different time range of flexibility.}},
  author       = {{Nord, Johan and Nyman, Per-Olof and Larsson, Gunilla and Drakenberg, Torbjörn}},
  issn         = {{1873-3468}},
  keywords     = {{dUTPase; Equine infectious anemia virus; Flexible C-terminus; Nuclear magnetic resonance; Nucleotide binding; Escherichia coli}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{228--232}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{FEBS Letters}},
  title        = {{The C-terminus of dUTPase: observation on flexibility using NMR}},
  url          = {{http://dx.doi.org/10.1016/S0014-5793(01)02257-8}},
  doi          = {{10.1016/S0014-5793(01)02257-8}},
  volume       = {{492}},
  year         = {{2001}},
}