Crystallization and preliminary X-ray analysis of human dUTPase
(1996) In Acta Crystallographica. Section D: Biological Crystallography D52(5). p.1039-1040- Abstract
- Human dUTPase, expressed in Escherichia coli, has been crystallized. Single crystals were obtained by the vapour-diffusion technique using 2-propanol and PEG 4000 as precipitants. The enzyme was co-crystallized with the substrate dUTP and a metal chelator EDTA to prevent hydrolysis of the substrate. The crystals belong to the orthorombic space group P212121 with cell dimensions a = 67.51, b = 68.26 and c = 91.00 Å. The crystallographic asymmetric unit contains one trimer of identical subunits.
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https://lup.lub.lu.se/record/126300
- author
- Larsson, G ; Nyman, Per-Olof LU and Svensson, L A
- organization
- publishing date
- 1996
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Acta Crystallographica. Section D: Biological Crystallography
- volume
- D52
- issue
- 5
- pages
- 1039 - 1040
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- scopus:0030513996
- ISSN
- 1399-0047
- DOI
- 10.1107/S0907444996003277
- language
- English
- LU publication?
- yes
- id
- 0aa1f215-5b3c-4bf1-9041-e1aa1c2d2f1a (old id 126300)
- date added to LUP
- 2016-04-01 16:27:18
- date last changed
- 2022-01-28 19:50:27
@article{0aa1f215-5b3c-4bf1-9041-e1aa1c2d2f1a, abstract = {{Human dUTPase, expressed in Escherichia coli, has been crystallized. Single crystals were obtained by the vapour-diffusion technique using 2-propanol and PEG 4000 as precipitants. The enzyme was co-crystallized with the substrate dUTP and a metal chelator EDTA to prevent hydrolysis of the substrate. The crystals belong to the orthorombic space group P212121 with cell dimensions a = 67.51, b = 68.26 and c = 91.00 Å. The crystallographic asymmetric unit contains one trimer of identical subunits.}}, author = {{Larsson, G and Nyman, Per-Olof and Svensson, L A}}, issn = {{1399-0047}}, language = {{eng}}, number = {{5}}, pages = {{1039--1040}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Acta Crystallographica. Section D: Biological Crystallography}}, title = {{Crystallization and preliminary X-ray analysis of human dUTPase}}, url = {{http://dx.doi.org/10.1107/S0907444996003277}}, doi = {{10.1107/S0907444996003277}}, volume = {{D52}}, year = {{1996}}, }