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Selective immunolesion of cholinergic neurons leads to long-term changes in 5-HT2A receptor levels in hippocampus and frontal cortex.

Severino, Maurizio LU ; Pedersen, AF ; Trajkovska, V ; Christensen, E ; Lohals, R ; Veng, LM ; Knudsen, GM and Aznar, S (2007) In Neuroscience Letters 428(1). p.47-51
Abstract
Although loss of cholinergic neurons in the basal forebrain is considered a key initial feature in Alzheimer's disease (AD), changes in other transmitter systems, including serotonin and 5-HT2A receptors, are also associated with early AD. The aim of this study was to investigate whether elimination of the cholinergic neurons in the basal forebrain directly affects 5-HT2A receptor levels. For this purpose intraventricular injection of the selective immunotoxin 192 IgG-Saporin was given to rats in doses of either 2.5 or 5 μg. The rats were sacrificed after 1, 2, 4 and 20 weeks. 5-HT2A protein levels were determined by western techniques in frontal cortex and hippocampus. A significant 70% downregulation in frontal cortex and a 100%... (More)
Although loss of cholinergic neurons in the basal forebrain is considered a key initial feature in Alzheimer's disease (AD), changes in other transmitter systems, including serotonin and 5-HT2A receptors, are also associated with early AD. The aim of this study was to investigate whether elimination of the cholinergic neurons in the basal forebrain directly affects 5-HT2A receptor levels. For this purpose intraventricular injection of the selective immunotoxin 192 IgG-Saporin was given to rats in doses of either 2.5 or 5 μg. The rats were sacrificed after 1, 2, 4 and 20 weeks. 5-HT2A protein levels were determined by western techniques in frontal cortex and hippocampus. A significant 70% downregulation in frontal cortex and a 100% upregulation in hippocampus of 5-HT2A receptor levels were observed 20 weeks after the cholinergic lesion when using the highest dose of 192 IgG-Saporin. Our results show that cholinergic deafferentation leads to decreased frontal cortex and increased hippoca (Less)
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author
; ; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
subject
in
Neuroscience Letters
volume
428
issue
1
pages
47 - 51
publisher
Elsevier
external identifiers
  • scopus:35448937166
ISSN
0304-3940
DOI
10.1016/j.neulet.2007.09.026
language
English
LU publication?
no
id
b56423ec-c87e-4e64-852c-5bde9806c212 (old id 1298974)
date added to LUP
2016-04-04 10:11:37
date last changed
2022-01-29 19:53:46
@article{b56423ec-c87e-4e64-852c-5bde9806c212,
  abstract     = {{Although loss of cholinergic neurons in the basal forebrain is considered a key initial feature in Alzheimer's disease (AD), changes in other transmitter systems, including serotonin and 5-HT2A receptors, are also associated with early AD. The aim of this study was to investigate whether elimination of the cholinergic neurons in the basal forebrain directly affects 5-HT2A receptor levels. For this purpose intraventricular injection of the selective immunotoxin 192 IgG-Saporin was given to rats in doses of either 2.5 or 5 μg. The rats were sacrificed after 1, 2, 4 and 20 weeks. 5-HT2A protein levels were determined by western techniques in frontal cortex and hippocampus. A significant 70% downregulation in frontal cortex and a 100% upregulation in hippocampus of 5-HT2A receptor levels were observed 20 weeks after the cholinergic lesion when using the highest dose of 192 IgG-Saporin. Our results show that cholinergic deafferentation leads to decreased frontal cortex and increased hippoca}},
  author       = {{Severino, Maurizio and Pedersen, AF and Trajkovska, V and Christensen, E and Lohals, R and Veng, LM and Knudsen, GM and Aznar, S}},
  issn         = {{0304-3940}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{47--51}},
  publisher    = {{Elsevier}},
  series       = {{Neuroscience Letters}},
  title        = {{Selective immunolesion of cholinergic neurons leads to long-term changes in 5-HT2A receptor levels in hippocampus and frontal cortex.}},
  url          = {{http://dx.doi.org/10.1016/j.neulet.2007.09.026}},
  doi          = {{10.1016/j.neulet.2007.09.026}},
  volume       = {{428}},
  year         = {{2007}},
}