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Characterization of the human CREB3L2 gene promoter

Panagopoulos, Ioannis LU and Mertens, Fredrik LU (2009) In Oncology Reports 21(3). p.615-624
Abstract

CREB3L2 encodes a member of the CREB3 family of transcription factors. We characterized its promoter region, showing that it is asymmetrically bidirectional, also driving the expression of a variant of AKR1D1. It has a CRE binding site which is conserved among mammalians; removal or alteration of it resulted in reduced promoter activity. When transiently transfecting the HEK293 cell line with constructs with partially deleted promoter regions, 5' deletions beyond 1058-bp upstream of the transcription starting site resulted in successive reduction of the activity. The inclusion of the untranslated part of CREB3L2 exon 1 strongly inhibited the promoter activity. Forskolin resulted in a decreased reporter activity, whereas phorbol... (More)

CREB3L2 encodes a member of the CREB3 family of transcription factors. We characterized its promoter region, showing that it is asymmetrically bidirectional, also driving the expression of a variant of AKR1D1. It has a CRE binding site which is conserved among mammalians; removal or alteration of it resulted in reduced promoter activity. When transiently transfecting the HEK293 cell line with constructs with partially deleted promoter regions, 5' deletions beyond 1058-bp upstream of the transcription starting site resulted in successive reduction of the activity. The inclusion of the untranslated part of CREB3L2 exon 1 strongly inhibited the promoter activity. Forskolin resulted in a decreased reporter activity, whereas phorbol 12-myristate 13-acetate increased the promoter activity irrespective of the status of the CRE binding site. The presence of the CRE site indicates autoregulation of CREB3L2 and/or regulation via other members of the CREB3 family or a variety of bZIP transcription factors.

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publication status
published
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keywords
Base Sequence, Basic-Leucine Zipper Transcription Factors, Gene Expression Regulation, Humans, Molecular Sequence Data, Mutagenesis, Site-Directed, Promoter Regions, Genetic, Reverse Transcriptase Polymerase Chain Reaction, Sequence Homology, Nucleic Acid, Journal Article, Research Support, Non-U.S. Gov't
in
Oncology Reports
volume
21
issue
3
pages
10 pages
publisher
Spandidos Publications
external identifiers
  • wos:000263584000007
  • pmid:19212619
  • scopus:64849107204
  • pmid:19212619
ISSN
1791-2431
DOI
10.3892/or_00000264
language
English
LU publication?
yes
id
946ee665-f664-4c15-b18f-83ebf6766f78 (old id 1302653)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19212619?dopt=Abstract
date added to LUP
2016-04-04 09:04:41
date last changed
2022-02-28 06:16:13
@article{946ee665-f664-4c15-b18f-83ebf6766f78,
  abstract     = {{<p>CREB3L2 encodes a member of the CREB3 family of transcription factors. We characterized its promoter region, showing that it is asymmetrically bidirectional, also driving the expression of a variant of AKR1D1. It has a CRE binding site which is conserved among mammalians; removal or alteration of it resulted in reduced promoter activity. When transiently transfecting the HEK293 cell line with constructs with partially deleted promoter regions, 5' deletions beyond 1058-bp upstream of the transcription starting site resulted in successive reduction of the activity. The inclusion of the untranslated part of CREB3L2 exon 1 strongly inhibited the promoter activity. Forskolin resulted in a decreased reporter activity, whereas phorbol 12-myristate 13-acetate increased the promoter activity irrespective of the status of the CRE binding site. The presence of the CRE site indicates autoregulation of CREB3L2 and/or regulation via other members of the CREB3 family or a variety of bZIP transcription factors.</p>}},
  author       = {{Panagopoulos, Ioannis and Mertens, Fredrik}},
  issn         = {{1791-2431}},
  keywords     = {{Base Sequence; Basic-Leucine Zipper Transcription Factors; Gene Expression Regulation; Humans; Molecular Sequence Data; Mutagenesis, Site-Directed; Promoter Regions, Genetic; Reverse Transcriptase Polymerase Chain Reaction; Sequence Homology, Nucleic Acid; Journal Article; Research Support, Non-U.S. Gov't}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{615--624}},
  publisher    = {{Spandidos Publications}},
  series       = {{Oncology Reports}},
  title        = {{Characterization of the human CREB3L2 gene promoter}},
  url          = {{http://dx.doi.org/10.3892/or_00000264}},
  doi          = {{10.3892/or_00000264}},
  volume       = {{21}},
  year         = {{2009}},
}