Phylogenetic analyses and expression studies reveal two distinct groups of calreticulin isoforms in higher plants.
(2003) In Plant Physiology 133(3). p.1385-1396- Abstract
- Calreticulin (CRT) is a multifunctional protein mainly localized to the endoplasmic reticulum in eukaryotic cells. Here, we present the first analysis, to our knowledge, of evolutionary diversity and expression profiling among different plant CRT isoforms. Phylogenetic studies and expression analysis show that higher plants contain two distinct groups of CRTs: a CRT1/CRT2 group and a CRT3 group. To corroborate the existence of these isoform groups, we cloned a putative CRT3 ortholog from Brassica rapa. The CRT3 gene appears to be most closely related to the ancestral CRT gene in higher plants. Distinct tissue-dependent expression patterns and stress-related regulation were observed for the isoform groups. Furthermore, analysis of... (More)
- Calreticulin (CRT) is a multifunctional protein mainly localized to the endoplasmic reticulum in eukaryotic cells. Here, we present the first analysis, to our knowledge, of evolutionary diversity and expression profiling among different plant CRT isoforms. Phylogenetic studies and expression analysis show that higher plants contain two distinct groups of CRTs: a CRT1/CRT2 group and a CRT3 group. To corroborate the existence of these isoform groups, we cloned a putative CRT3 ortholog from Brassica rapa. The CRT3 gene appears to be most closely related to the ancestral CRT gene in higher plants. Distinct tissue-dependent expression patterns and stress-related regulation were observed for the isoform groups. Furthermore, analysis of posttranslational modifications revealed differences in the glycosylation status among members within the CRT1/CRT2 isoform group. Based on evolutionary relationship, a new nomenclature for plant CRTs is suggested. The presence of two distinct CRT isoform groups, with distinct expression patterns and posttranslational modifications, supports functional specificity among plant CRTs and could account for the multiple functional roles assigned to CRTs. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/132700
- author
- Persson, Staffan LU ; Rosenquist, Magnus LU ; Svensson, Karin ; Galvao, R ; Boss, W F and Sommarin, Marianne LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Plant Physiology
- volume
- 133
- issue
- 3
- pages
- 1385 - 1396
- publisher
- American Society of Plant Biologists
- external identifiers
-
- wos:000186644600043
- pmid:14563927
- scopus:0344961215
- ISSN
- 1532-2548
- DOI
- 10.1104/pp.103.024943
- language
- English
- LU publication?
- yes
- id
- e21a1a03-2e56-4bc4-843f-ecd942152f38 (old id 132700)
- date added to LUP
- 2016-04-01 11:39:01
- date last changed
- 2022-02-03 02:28:55
@article{e21a1a03-2e56-4bc4-843f-ecd942152f38, abstract = {{Calreticulin (CRT) is a multifunctional protein mainly localized to the endoplasmic reticulum in eukaryotic cells. Here, we present the first analysis, to our knowledge, of evolutionary diversity and expression profiling among different plant CRT isoforms. Phylogenetic studies and expression analysis show that higher plants contain two distinct groups of CRTs: a CRT1/CRT2 group and a CRT3 group. To corroborate the existence of these isoform groups, we cloned a putative CRT3 ortholog from Brassica rapa. The CRT3 gene appears to be most closely related to the ancestral CRT gene in higher plants. Distinct tissue-dependent expression patterns and stress-related regulation were observed for the isoform groups. Furthermore, analysis of posttranslational modifications revealed differences in the glycosylation status among members within the CRT1/CRT2 isoform group. Based on evolutionary relationship, a new nomenclature for plant CRTs is suggested. The presence of two distinct CRT isoform groups, with distinct expression patterns and posttranslational modifications, supports functional specificity among plant CRTs and could account for the multiple functional roles assigned to CRTs.}}, author = {{Persson, Staffan and Rosenquist, Magnus and Svensson, Karin and Galvao, R and Boss, W F and Sommarin, Marianne}}, issn = {{1532-2548}}, language = {{eng}}, number = {{3}}, pages = {{1385--1396}}, publisher = {{American Society of Plant Biologists}}, series = {{Plant Physiology}}, title = {{Phylogenetic analyses and expression studies reveal two distinct groups of calreticulin isoforms in higher plants.}}, url = {{http://dx.doi.org/10.1104/pp.103.024943}}, doi = {{10.1104/pp.103.024943}}, volume = {{133}}, year = {{2003}}, }