DRhoGEF2 regulates actin organization and contractility in the Drosophila blastoderm embryo.
(2005) In Journal of Cell Biology 168(4). p.575-585- Abstract
- Morphogenesis of the Drosophila melanogaster embryo is associated with a dynamic reorganization of the actin cytoskeleton that is mediated by small GTPases of the Rho family. Often, Rho1 controls different aspects of cytoskeletal function in parallel, requiring a complex level of regulation. We show that the guanine triphosphate (GTP) exchange factor DRhoGEF2 is apically localized in epithelial cells throughout embryogenesis. We demonstrate that DRhoGEF2, which has previously been shown to regulate cell shape changes during gastrulation, recruits Rho1 to actin rings and regulates actin distribution and actomyosin contractility during nuclear divisions, pole cell formation, and cellularization of syncytial blastoderm embryos. We propose... (More)
- Morphogenesis of the Drosophila melanogaster embryo is associated with a dynamic reorganization of the actin cytoskeleton that is mediated by small GTPases of the Rho family. Often, Rho1 controls different aspects of cytoskeletal function in parallel, requiring a complex level of regulation. We show that the guanine triphosphate (GTP) exchange factor DRhoGEF2 is apically localized in epithelial cells throughout embryogenesis. We demonstrate that DRhoGEF2, which has previously been shown to regulate cell shape changes during gastrulation, recruits Rho1 to actin rings and regulates actin distribution and actomyosin contractility during nuclear divisions, pole cell formation, and cellularization of syncytial blastoderm embryos. We propose that DRhoGEF2 activity coordinates contractile actomyosin forces throughout morphogenesis in Drosophila by regulating the association of myosin with actin to form contractile cables. Our results support the hypothesis that specific aspects of Rho1 function are regulated by specific GTP exchange factors. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/133858
- author
- Padash, Mojgan LU ; Rogers, Stephen and Häcker, Udo LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Cell Biology
- volume
- 168
- issue
- 4
- pages
- 575 - 585
- publisher
- Rockefeller University Press
- external identifiers
-
- wos:000227101100024
- pmid:15699213
- scopus:13944278366
- ISSN
- 0021-9525
- DOI
- 10.1083/jcb.200407124
- language
- English
- LU publication?
- yes
- id
- f4740509-5ccd-4360-a4b0-46ac4590311a (old id 133858)
- date added to LUP
- 2016-04-01 12:36:31
- date last changed
- 2022-01-27 07:22:58
@article{f4740509-5ccd-4360-a4b0-46ac4590311a, abstract = {{Morphogenesis of the Drosophila melanogaster embryo is associated with a dynamic reorganization of the actin cytoskeleton that is mediated by small GTPases of the Rho family. Often, Rho1 controls different aspects of cytoskeletal function in parallel, requiring a complex level of regulation. We show that the guanine triphosphate (GTP) exchange factor DRhoGEF2 is apically localized in epithelial cells throughout embryogenesis. We demonstrate that DRhoGEF2, which has previously been shown to regulate cell shape changes during gastrulation, recruits Rho1 to actin rings and regulates actin distribution and actomyosin contractility during nuclear divisions, pole cell formation, and cellularization of syncytial blastoderm embryos. We propose that DRhoGEF2 activity coordinates contractile actomyosin forces throughout morphogenesis in Drosophila by regulating the association of myosin with actin to form contractile cables. Our results support the hypothesis that specific aspects of Rho1 function are regulated by specific GTP exchange factors.}}, author = {{Padash, Mojgan and Rogers, Stephen and Häcker, Udo}}, issn = {{0021-9525}}, language = {{eng}}, number = {{4}}, pages = {{575--585}}, publisher = {{Rockefeller University Press}}, series = {{Journal of Cell Biology}}, title = {{DRhoGEF2 regulates actin organization and contractility in the Drosophila blastoderm embryo.}}, url = {{https://lup.lub.lu.se/search/files/2992697/624398.pdf}}, doi = {{10.1083/jcb.200407124}}, volume = {{168}}, year = {{2005}}, }