Gene expression profiling demonstrates that TGF-{beta}1 signals exclusively through receptor complexes involving Alk5 and identifies targets of TGF-{beta} signaling.
Karlsson, Göran LU ; Liu, Yingchun LU ; Larsson, Jonas LU ; Goumans, Marie-Jose ; Lee, Ju-Seog ; Thorgeirsson, Snorri S ; Ringnér, Markus LU
and Karlsson, Stefan
LU
(2005)
In Physiological Genomics
21(3).
p.396-403
- Abstract
- Transforming growth factor-β 1 (TGF-β) regulates cellular functions like proliferation, differentiation, and apoptosis. On the cell surface, TGF-β binds to receptor complexes consisting of TGF-β receptor type II (Tβ RII) and activin-like kinase receptor-5 (Alk5), and the downstream signaling is transduced by Smad and MAPK proteins. Recent data have shown that alternative receptor combinations aside from the classical pairing of Tβ RII/Alk5 can be relevant for TGF-β signaling. We have screened for alternative receptors for TGF-β and also for gene targets of TGF-β signaling, by performing functional assays and microarray analysis in murine embryonic fibroblast (MEF) cell lines lacking Alk5. Data from TGF-β-stimulated Alk5(-/-) cells show... (More)
- Transforming growth factor-β 1 (TGF-β) regulates cellular functions like proliferation, differentiation, and apoptosis. On the cell surface, TGF-β binds to receptor complexes consisting of TGF-β receptor type II (Tβ RII) and activin-like kinase receptor-5 (Alk5), and the downstream signaling is transduced by Smad and MAPK proteins. Recent data have shown that alternative receptor combinations aside from the classical pairing of Tβ RII/Alk5 can be relevant for TGF-β signaling. We have screened for alternative receptors for TGF-β and also for gene targets of TGF-β signaling, by performing functional assays and microarray analysis in murine embryonic fibroblast (MEF) cell lines lacking Alk5. Data from TGF-β-stimulated Alk5(-/-) cells show them to be completely unaffected by TGF-β. Additionally, 465 downstream targets of Alk5 signaling were identified when comparing Alk5(-/-) or TGF-β-stimulated Alk5(+/+) MEFs with unstimulated Alk5(+/+) cells. Our results demonstrate that, in MEFs, TGF-β signals exclusively through complexes involving Alk5, and give insight to its downstream effector genes. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/134937
- author
- Karlsson, Göran
LU
; Liu, Yingchun
LU
; Larsson, Jonas
LU
; Goumans, Marie-Jose
; Lee, Ju-Seog
; Thorgeirsson, Snorri S
; Ringnér, Markus
LU
and Karlsson, Stefan
LU
- organization
- publishing date
- 2005-05-11
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- signal transduction, Smad, microarrays
- in
- Physiological Genomics
- volume
- 21
- issue
- 3
- pages
- 396 - 403
- publisher
- American Physiological Society
- external identifiers
-
- wos:000229021500013
- pmid:15769904
- scopus:21244489290
- pmid:15769904
- ISSN
- 1094-8341
- DOI
- 10.1152/physiolgenomics.00303.2004
- language
- English
- LU publication?
- yes
- id
- 6f7d20f4-c646-4fb0-b086-a32f176797cd (old id 134937)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=15769904&dopt=Abstract
- date added to LUP
- 2016-04-01 11:39:27
- date last changed
- 2025-04-04 15:01:36
@article{6f7d20f4-c646-4fb0-b086-a32f176797cd,
abstract = {{Transforming growth factor-β 1 (TGF-β) regulates cellular functions like proliferation, differentiation, and apoptosis. On the cell surface, TGF-β binds to receptor complexes consisting of TGF-β receptor type II (Tβ RII) and activin-like kinase receptor-5 (Alk5), and the downstream signaling is transduced by Smad and MAPK proteins. Recent data have shown that alternative receptor combinations aside from the classical pairing of Tβ RII/Alk5 can be relevant for TGF-β signaling. We have screened for alternative receptors for TGF-β and also for gene targets of TGF-β signaling, by performing functional assays and microarray analysis in murine embryonic fibroblast (MEF) cell lines lacking Alk5. Data from TGF-β-stimulated Alk5(-/-) cells show them to be completely unaffected by TGF-β. Additionally, 465 downstream targets of Alk5 signaling were identified when comparing Alk5(-/-) or TGF-β-stimulated Alk5(+/+) MEFs with unstimulated Alk5(+/+) cells. Our results demonstrate that, in MEFs, TGF-β signals exclusively through complexes involving Alk5, and give insight to its downstream effector genes.}},
author = {{Karlsson, Göran and Liu, Yingchun and Larsson, Jonas and Goumans, Marie-Jose and Lee, Ju-Seog and Thorgeirsson, Snorri S and Ringnér, Markus and Karlsson, Stefan}},
issn = {{1094-8341}},
keywords = {{signal transduction; Smad; microarrays}},
language = {{eng}},
month = {{05}},
number = {{3}},
pages = {{396--403}},
publisher = {{American Physiological Society}},
series = {{Physiological Genomics}},
title = {{Gene expression profiling demonstrates that TGF-{beta}1 signals exclusively through receptor complexes involving Alk5 and identifies targets of TGF-{beta} signaling.}},
url = {{http://dx.doi.org/10.1152/physiolgenomics.00303.2004}},
doi = {{10.1152/physiolgenomics.00303.2004}},
volume = {{21}},
year = {{2005}},
}